Construct: ORF TRCN0000467064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015219.1_s317c1
- Derived from:
- ccsbBroadEn_00526
- DNA Barcode:
- TCGGACAAAATCTCATTTTCATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EZH2 (2146)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | NM_004456.5 | 100% | 100% | |
| 2 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515884.2 | 100% | 100% | |
| 3 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | NM_001203247.2 | 99.3% | 99.2% | 892_893insGTAAGTGCAATTATT |
| 4 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515883.2 | 98.9% | 98.9% | 1_24del |
| 5 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_017011817.2 | 98.9% | 98.9% | 1_24del |
| 6 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515887.3 | 98.8% | 98.8% | 219_220ins27 |
| 7 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515888.2 | 98.8% | 98.8% | 219_220ins27 |
| 8 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_005249962.4 | 98.2% | 98.1% | 1_24del;916_917insGTAAGTGCAATTATT |
| 9 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | NM_001203248.2 | 98.1% | 98% | 219_220ins27;865_866insGTAAGTGCAATTATT |
| 10 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515885.2 | 97.7% | 97.7% | 1_24del;243_244ins27 |
| 11 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_005249963.4 | 97.1% | 96.9% | 1_24del;243_244ins27;889_890insGTAAGTGCAATTATT |
| 12 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515894.2 | 94.8% | 94.8% | 245_246ins117 |
| 13 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | NM_152998.3 | 94.1% | 94% | 245_246ins117;775_776insGTAAGTGCAATTATT |
| 14 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515890.2 | 93.8% | 93.8% | 1_24del;269_270ins117 |
| 15 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_017011820.2 | 93.6% | 93.6% | 219_220ins144 |
| 16 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515892.2 | 93.4% | 93.2% | 1_24del;1570_1571ins126 |
| 17 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515893.2 | 93.1% | 93% | 1_24del;269_270ins117;799_800insGTAAGTGCAATTATT |
| 18 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_017011819.1 | 92.7% | 92.4% | 1_24del;916_917insGTAAGTGCAATTATT;1555_1556ins126 |
| 19 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515895.2 | 92.6% | 92.6% | 1_24del;243_244ins144 |
| 20 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515891.3 | 92.6% | 90.9% | (many diffs) |
| 21 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | NM_001203249.2 | 92.5% | 92.2% | 219_220ins27;865_866insGTAAGTGCAATTATT;1504_1505ins126 |
| 22 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_024446680.1 | 91.9% | 90.1% | (many diffs) |
| 23 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_005249964.4 | 91.5% | 91.3% | (many diffs) |
| 24 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515896.2 | 87.6% | 87.3% | (many diffs) |
| 25 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515889.2 | 87.6% | 86.6% | (many diffs) |
| 26 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515886.2 | 86% | 87.2% | (many diffs) |
| 27 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515897.2 | 85.4% | 85.4% | 0_1ins327 |
| 28 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515898.2 | 85.4% | 85.4% | 0_1ins327 |
| 29 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_017011818.1 | 85.4% | 86.4% | (many diffs) |
| 30 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_017011821.1 | 84.8% | 84.6% | 0_1ins327;565_566insGTAAGTGCAATTATT |
| 31 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515899.3 | 66.1% | 66.2% | 1_24del;1531delC;1533_1534ins745 |
| 32 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XM_011515901.3 | 55.3% | 54.1% | (many diffs) |
| 33 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XR_002956413.1 | 40.1% | (many diffs) | |
| 34 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XR_001744581.1 | 38.5% | 1_2639del;3878_3879ins265;4628_4893del | |
| 35 | human | 2146 | EZH2 | enhancer of zeste 2 polycom... | XR_002956414.1 | 37.4% | (many diffs) | |
| 36 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505520.3 | 92.5% | 98.2% | (many diffs) |
| 37 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505521.2 | 92.5% | 98.2% | (many diffs) |
| 38 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505526.3 | 92.5% | 98.2% | (many diffs) |
| 39 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | NM_007971.2 | 91.9% | 97.6% | (many diffs) |
| 40 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | NM_001146689.1 | 91.3% | 97% | (many diffs) |
| 41 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505523.1 | 90.7% | 96.4% | (many diffs) |
| 42 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505524.1 | 86.9% | 92.5% | (many diffs) |
| 43 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505525.1 | 85.1% | 90.6% | (many diffs) |
| 44 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505527.1 | 57.9% | 54.3% | (many diffs) |
| 45 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505528.1 | 57.9% | 54.3% | (many diffs) |
| 46 | mouse | 14056 | Ezh2 | enhancer of zeste 2 polycom... | XM_006505529.1 | 57.3% | 54.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2319
- ORF length:
- 2253
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccagactggg aagaaatctg agaagggacc agtttgttgg cggaagcgtg 121 taaaatcaga gtacatgcga ctgagacagc tcaagaggtt cagacgagct gatgaagtaa 181 agagtatgtt tagttccaat cgtcagaaaa ttttggaaag aacggaaatc ttaaaccaag 241 aatggaaaca gcgaaggata cagcctgtgc acatcctgac ttctgtgagc tcattgcgcg 301 ggactaggga gtgttcggtg accagtgact tggattttcc aacacaagtc atcccattaa 361 agactctgaa tgcagttgct tcagtaccca taatgtattc ttggtctccc ctacagcaga 421 attttatggt ggaagatgaa actgttttac ataacattcc ttatatggga gatgaagttt 481 tagatcagga tggtactttc attgaagaac taataaaaaa ttatgatggg aaagtacacg 541 gggatagaga atgtgggttt ataaatgatg aaatttttgt ggagttggtg aatgcccttg 601 gtcaatataa tgatgatgac gatgatgatg atggagacga tcctgaagaa agagaagaaa 661 agcagaaaga tctggaggat caccgagatg ataaagaaag ccgcccacct cggaaatttc 721 cttctgataa aatttttgaa gccatttcct caatgtttcc agataagggc acagcagaag 781 aactaaagga aaaatataaa gaactcaccg aacagcagct cccaggcgca cttcctcctg 841 aatgtacccc caacatagat ggaccaaatg ctaaatctgt tcagagagag caaagcttac 901 actcctttca tacgcttttc tgtaggcgat gttttaaata tgactgcttc ctacatcgta 961 agtgcaatta ttcttttcat gcaacaccca acacttataa gcggaagaac acagaaacag 1021 ctctagacaa caaaccttgt ggaccacagt gttaccagca tttggaggga gcaaaggagt 1081 ttgctgctgc tctcaccgct gagcggataa agaccccacc aaaacgtcca ggaggccgca 1141 gaagaggacg gcttcccaat aacagtagca ggcccagcac ccccaccatt aatgtgctgg 1201 aatcaaagga tacagacagt gatagggaag cagggactga aacgggggga gagaacaatg 1261 ataaagaaga agaagagaag aaagatgaaa cttcgagctc ctctgaagca aattctcggt 1321 gtcaaacacc aataaagatg aagccaaata ttgaacctcc tgagaatgtg gagtggagtg 1381 gtgctgaagc ctcaatgttt agagtcctca ttggcactta ctatgacaat ttctgtgcca 1441 ttgctaggtt aattgggacc aaaacatgta gacaggtgta tgagtttaga gtcaaagaat 1501 ctagcatcat agctccagct cccgctgagg atgtggatac tcctccaagg aaaaagaaga 1561 ggaaacaccg gttgtgggct gcacactgca gaaagataca gctgaaaaag gacggctcct 1621 ctaaccatgt ttacaactat caaccctgtg atcatccacg gcagccttgt gacagttcgt 1681 gcccttgtgt gatagcacaa aatttttgtg aaaagttttg tcaatgtagt tcagagtgtc 1741 aaaaccgctt tccgggatgc cgctgcaaag cacagtgcaa caccaagcag tgcccgtgct 1801 acctggctgt ccgagagtgt gaccctgacc tctgtcttac ttgtggagcc gctgaccatt 1861 gggacagtaa aaatgtgtcc tgcaagaact gcagtattca gcggggctcc aaaaagcatc 1921 tattgctGGC ACCATCTGAC GTGGCAGGCT GGGGGATTTT TATCAAAGAT CCTGTGCAGA 1981 AAAATGAATT CATCTCAGAA TACTGTGGAG AGATTATTTC TCAAGATGAA GCTGACAGAA 2041 GAGGGAAAGT GTATGATAAA TACATGTGCA GCTTTCTGTT CAACTTGAAC AATGATTTTG 2101 TGGTGGATGC AACCCGCAAG GGTAACAAAA TTCGTTTTGC AAATCATTCG GTAAATCCAA 2161 ACTGCTATGC AAAAGTTATG ATGGTTAACG GTGATCACAG GATAGGTATT TTTGCCAAGA 2221 GAGCCATCCA GACTGGCGAA GAGCTGTTTT TTGATTACAG ATACAGCCAG GCTGATGCCC 2281 TGAAGTATGT CGGCATCGAA AGAGAAATGG AAATCCCTTG CCCAACTTTC TTGTACAAAG 2341 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2401 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2461 ACGATCGGAC AAAATCTCAT TTTCATAAAC GCGTTAAGTC gacaatcaac ctctggatta 2521 caaaatttgt gaaagatt