Construct: ORF TRCN0000467072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003503.3_s317c1
- Derived from:
- ccsbBroadEn_14791
- DNA Barcode:
- CTGGAACAGATCCCTCCGACATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCG (5582)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5582 | PRKCG | protein kinase C gamma | NM_002739.5 | 99.9% | 100% | 285C>T |
| 2 | human | 5582 | PRKCG | protein kinase C gamma | NM_001316329.2 | 98% | 98% | 285C>T;2091_2130delinsG |
| 3 | mouse | 18752 | Prkcg | protein kinase C, gamma | NM_011102.4 | 89.4% | 98.9% | (many diffs) |
| 4 | mouse | 18752 | Prkcg | protein kinase C, gamma | NM_001291434.1 | 82.9% | 91.8% | (many diffs) |
| 5 | mouse | 18752 | Prkcg | protein kinase C, gamma | XM_006539637.2 | 49.9% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggtctgggc cccggcgtag gcgattcaga ggggggaccc cggcccctgt 121 tttgcagaaa gggggccctg aggcagaagg tggtccacga agtcaagagc cacaagttca 181 ccgctcgctt cttcaagcag cccaccttct gcagccactg caccgacttc atctggggta 241 tcggaaagca gggcctgcaa tgtcaagtct gcagctttgt ggttcatcga cgatgccacg 301 aatttgtgac cttcgagtgt ccaggcgctg ggaagggccc ccagacggat gacccccgga 361 acaaacacaa gttccgcctg catagctaca gcagccccac cttctgcgac cactgtggct 421 ccctcctcta cgggcttgtg caccagggca tgaaatgctc ctgctgcgag atgaacgtgc 481 accggcgctg tgtgcgtagc gtgccctccc tgtgcggtgt ggaccacacc gagcgccgcg 541 ggcgcctgca gctggagatc cgggctccca cagcagatga gatccacgta actgttggcg 601 aggcccgtaa cctaattcct atggacccca atggtctctc tgatccctat gtgaaactga 661 agctcatccc agaccctcgg aacctgacga aacagaagac ccgaacggtg aaagccacgc 721 taaaccctgt gtggaatgag acctttgtgt tcaacctgaa gccaggggat gtggagcgcc 781 ggctcagcgt ggaggtgtgg gactgggacc ggacctcccg caacgacttc atgggggcca 841 tgtcctttgg cgtctcggag ctgctcaagg cgcccgtgga tggctggtac aagttactga 901 accaggagga gggcgagtat tacaatgtgc cggtggccga tgctgacaac tgcagcctcc 961 tccagaagtt tgaggcttgt aactaccccc tggaattgta tgagcgggtg cggatgggcc 1021 cctcttcctc tcccatcccc tccccttccc ctagtcccac cgaccccaag cgctgcttct 1081 tcggggcgag tccaggacgc ctgcacatct ccgacttcag cttcctcatg gttctaggaa 1141 aaggcagttt tgggaaggtg atgctggccg agcgcagggg ctctgatgag ctctacgcca 1201 tcaagatctt gaaaaaggac gtgatcgtcc aggacgacga tgtggactgc acgctggtgg 1261 agaaacgtgt gctggcgctg gggggccggg gtcctggcgg ccggccccac ttcctcaccc 1321 agctccactc caccttccag accccggacc gcctgtattt cgtgatggag tacgtcaccg 1381 ggggagactt gatgtaccac attcaacagc tgggcaagtt taaggagccc catgcagcgt 1441 tctacgcggc agaaatcgct atcggcctct tcttccttca caatcagggc atcatctaca 1501 gggacctgaa gctggacaat gtgatgctgg atgctgaggg acacatcaag atcactgact 1561 ttggcatgtg taaggagaac gtcttccccg ggacgacaac ccgcaccttc tgcgggaccc 1621 cggactacat agccccggag atcattgcct accagcccta tgggaagtct gtcgattggt 1681 ggtcctttgg agttctgctg tatgagatgt tggcaggaca gcctcccttc gatggggagg 1741 acgaggagga gctgtttcag gccatcatgg aacaaactgt cacctacccC AAGTCGCTTT 1801 CCCGGGAAGC CGTGGCCATC TGCAAGGGGT TCCTGACCAA GCACCCAGGG AAGCGCCTGG 1861 GCTCAGGGCC TGATGGGGAA CCTACCATCC GTGCACATGG CTTTTTCCGC TGGATTGACT 1921 GGGAGCGGCT GGAACGATTG GAGATCCCGC CTCCTTTCAG ACCCCGCCCG TGTGGCCGCA 1981 GCGGCGAGAA CTTTGACAAG TTCTTCACGC GGGCGGCGCC AGCGCTGACC CCTCCAGACC 2041 GCCTAGTCCT GGCCAGCATC GACCAGGCCG ATTTCCAGGG CTTCACCTAC GTGAACCCCG 2101 ACTTCGTGCA CCCGGATGCC CGCAGCCCCA CCAGCCCAGT GCCTGTGCCC GTCATGTACC 2161 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GACTGGAACA GATCCCTCCG ACATAAACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt