Transcript: Human NM_002739.5

Homo sapiens protein kinase C gamma (PRKCG), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
PRKCG (5582)
Length:
3149
CDS:
299..2392

Additional Resources:

NCBI RefSeq record:
NM_002739.5
NBCI Gene record:
PRKCG (5582)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146492 CTCTACGGGCTTGTGCACCA pXPR_003 GGG 377 18% 4 0.2603 PRKCG PRKCG 76899
2 BRDN0001147740 ATTCCACACAGGGTTTAGCG pXPR_003 TGG 655 31% 6 -0.0543 PRKCG PRKCG 76898
3 BRDN0001146495 CGAGTATTACAATGTGCCGG pXPR_003 TGG 865 41% 8 -0.1309 PRKCG PRKCG 76900
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002739.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199544 CCTGTCCCATTCTAGATCCTG pLKO.1 2476 3UTR 100% 2.640 3.696 N PRKCG n/a
2 TRCN0000010693 GCTTTGTGGTTCATCGACGAT pLKO.1 507 CDS 100% 2.640 3.696 N PRKCG n/a
3 TRCN0000199422 CGAGACTCCATCTTGGTAGTT pLKO.1 2703 3UTR 100% 4.950 3.960 N PRKCG n/a
4 TRCN0000002326 CCGATATTCTCCCTGACCTTA pLKO.1 2614 3UTR 100% 4.950 3.465 N PRKCG n/a
5 TRCN0000195400 CTACGCCATCAAGATCTTGAA pLKO.1 1426 CDS 100% 4.950 3.465 N PRKCG n/a
6 TRCN0000199917 GCCTCCACTCTAGTTCTAGAT pLKO.1 2824 3UTR 100% 4.950 3.465 N PRKCG n/a
7 TRCN0000002325 GTGGAATGAGACCTTTGTGTT pLKO.1 964 CDS 100% 4.950 3.465 N PRKCG n/a
8 TRCN0000002324 TCTGTCGATTGGTGGTCCTTT pLKO.1 1901 CDS 100% 4.950 3.465 N PRKCG n/a
9 TRCN0000002327 CAATGGTCTCTCTGATCCCTA pLKO.1 862 CDS 100% 2.640 1.848 N PRKCG n/a
10 TRCN0000199230 CCTCGGAACCTGACGAAACAG pLKO.1 908 CDS 100% 1.650 1.155 N PRKCG n/a
11 TRCN0000197203 GCGAGAACTTTGACAAGTTCT pLKO.1 2217 CDS 100% 0.495 0.347 N PRKCG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002739.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14791 pDONR223 0% 99.9% 100% None 285C>T n/a
2 ccsbBroad304_14791 pLX_304 0% 99.9% 100% V5 285C>T n/a
3 TRCN0000467072 CTGGAACAGATCCCTCCGACATAA pLX_317 17.9% 99.9% 100% V5 285C>T n/a
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