Construct: ORF TRCN0000467083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000787.1_s317c1
- Derived from:
- ccsbBroadEn_14018
- DNA Barcode:
- CGCCGCCAACGAATGTACATCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP29 (9411)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467083
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | NM_001328666.2 | 31% | 30.5% | (many diffs) |
| 2 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | NM_001328664.2 | 30.8% | 30.3% | (many diffs) |
| 3 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | NM_004815.4 | 30.8% | 30.3% | (many diffs) |
| 4 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | XM_011542439.2 | 30.8% | 30.3% | (many diffs) |
| 5 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | NM_001328665.2 | 25.5% | 25% | (many diffs) |
| 6 | human | 9411 | ARHGAP29 | Rho GTPase activating prote... | NM_001328667.2 | 25.5% | 25% | (many diffs) |
| 7 | mouse | 214137 | Arhgap29 | Rho GTPase activating prote... | NM_172525.2 | 26.8% | 26.8% | (many diffs) |
| 8 | mouse | 214137 | Arhgap29 | Rho GTPase activating prote... | XM_006501288.3 | 26.8% | 26.8% | (many diffs) |
| 9 | mouse | 214137 | Arhgap29 | Rho GTPase activating prote... | XM_006501289.3 | 22.1% | 22.4% | (many diffs) |
| 10 | mouse | 214137 | Arhgap29 | Rho GTPase activating prote... | XM_006501290.1 | 22.1% | 22.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgctcacaaa cagaaaaaga caaagaaaaa acgtgcttgg gcatcaggtc 121 aactctctac tgatattaca acttctgaaa tggggctcaa gtccttaagt tccaactcta 181 tttttgatcc ggattacatc aaggagttgg tgaatgatat caggaagttc tcccacatgt 241 tactatattt gaaanaagcc atattttcag actgttttaa agaagttatt catatacgtc 301 tagaggaact gctccgtgtt ttaaagtcta taatgaataa acatcagaac ctcaattctg 361 ttgatcttca aaatgctgca gaaatgctca ctgcaaaagt gaaagctgtg aacttcacag 421 aagttaatga agaaaacaaa aacgatctct tccaggaagt gttttcttct attgaaactt 481 tggcatttac ctttggaaat atccttacaa acttccttat gggagatgta ggcaatgatt 541 cattattgcg actgcctgtt tctcgagaaa ctaagtcgtt tgaaaatgtt tctgtggaat 601 cagtggactc atccagtgaa aaaggaaatt tttccccttt agaactagac aacgtgctgt 661 taaagaacac tgactctatc gagctggctt tgtcatatgc taaaacttgg tcaaaatata 721 ctaagaacat agtttcatgg gttgaaaaaa agcttaactt ggaattggag tccactagaa 781 atatggtcaa gttggcagag gcaactagaa ctaacattgg aattcaggag ttcatgccac 841 tgcagtctct gtttactaat gctcttctta atgatataga aagcagtcac cttttacaac 901 aaacaattgc agctctccag gctaacaaat ttgtgcagcc tctacttGGA AGGAAAAATG 961 AAATGGAAAA ACAAAGGAAA GAAATAAAAG AGCTTTGGAA ACAGGAGCAA AATAAAATGC 1021 TTGAAGCAGA GAATGCTCTC AAAAAGGCAA AATTATTATG CATGCAACGT CAAGATGAAT 1081 ATGAGAAAGC AAAGTCTTCC ATGTTTCGTG CAGAAGAGGA GCATCTGTCT TCAAGTGGCG 1141 GATTAGCAAA AAATCTCAAC AAGCAACTAG AAAAAAAGCG AAGGTTGGAA GAGGAGGCTC 1201 TCCAAAAAGT AACGATCTTT TTCTTTTTTA TTTGCAAGTT GAATTACCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA CGCCGCCAAC GAATGTACAT CACTACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt