Construct: ORF TRCN0000467114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002710.1_s317c1
- Derived from:
- ccsbBroadEn_01468
- DNA Barcode:
- ATCCATATCAAGTGAAAACGTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTKN (6242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6242 | RTKN | rhotekin | NM_001015055.2 | 100% | 100% | |
| 2 | human | 6242 | RTKN | rhotekin | NM_033046.2 | 96.1% | 92.5% | (many diffs) |
| 3 | human | 6242 | RTKN | rhotekin | XM_017004635.2 | 96.1% | 92.5% | (many diffs) |
| 4 | human | 6242 | RTKN | rhotekin | NM_001015056.1 | 91.1% | 91.1% | 0_1ins150 |
| 5 | human | 6242 | RTKN | rhotekin | XM_005264478.3 | 84% | 81.3% | (many diffs) |
| 6 | human | 6242 | RTKN | rhotekin | XM_005264479.4 | 82.1% | 81.5% | (many diffs) |
| 7 | human | 6242 | RTKN | rhotekin | XM_017004636.2 | 80.2% | 74.2% | (many diffs) |
| 8 | human | 6242 | RTKN | rhotekin | XM_017004638.2 | 76.9% | 76.9% | 0_1ins390 |
| 9 | human | 6242 | RTKN | rhotekin | XM_017004637.2 | 75.2% | 72.5% | (many diffs) |
| 10 | human | 6242 | RTKN | rhotekin | XR_939706.1 | 73.7% | (many diffs) | |
| 11 | human | 6242 | RTKN | rhotekin | XM_017004639.1 | 73.2% | 72.6% | (many diffs) |
| 12 | human | 6242 | RTKN | rhotekin | XR_939705.2 | 72.1% | (many diffs) | |
| 13 | human | 6242 | RTKN | rhotekin | XR_939704.3 | 71.2% | 1_82del;1168_1169ins169;1603_1965del | |
| 14 | human | 6242 | RTKN | rhotekin | XM_017004640.2 | 61.1% | 58.3% | (many diffs) |
| 15 | human | 6242 | RTKN | rhotekin | XR_001738881.2 | 59.1% | (many diffs) | |
| 16 | mouse | 20166 | Rtkn | rhotekin | NM_001136227.1 | 87.5% | 86.7% | (many diffs) |
| 17 | mouse | 20166 | Rtkn | rhotekin | NM_009106.2 | 83.5% | 80.2% | (many diffs) |
| 18 | mouse | 20166 | Rtkn | rhotekin | NM_133641.2 | 83.5% | 80.2% | (many diffs) |
| 19 | mouse | 20166 | Rtkn | rhotekin | XM_006505806.3 | 79.2% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1755
- ORF length:
- 1689
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctcccgaaac caccggagcc gggtcaccgt ggccaggggc tccgccctgg 121 agatggagtt caaacgcggc cgcttccgac tcagcctctt cagcgacctg cccgaggaca 181 cggagttgca gaggaagcta gaccatgaga tccggatgag ggaaggggcc tgtaagctgc 241 tggcagcctg ctcccagcga gagcaggctc tggaggccac caagagcctg ctagtgtgca 301 acagccgcat cctcagctac atgggcgagc tgcagcggcg caaggaggcg caggtgctgg 361 ggaagacaag ccggcggcct tctgacagtg gcccgcccgc tgagcgctcc ccctgccgcg 421 gccgggtctg catctctgac ctccggattc cactcatgtg gaaggacaca gaatatttca 481 agaacaaagg tgacttgcac cgctgggctg tgttcctgct gctgcagctg ggggaacaca 541 tccaggacac agagatgatc ctagtggaca ggaccctcac agacatctcc tttcagagca 601 atgtgctctt cgctgaggcg gggccagact ttgaactgcg gttagagctg tatggggcct 661 gtgtggaaga agagggggcc ctgactggcg gccccaagag gcttgccacc aaactcagca 721 gctccctggg ccgctcctca gggaggcgtg tccgggcatc gctggacagt gctgggggtt 781 cagggagcag tcccatcttg ctccccaccc cagttgttgg tggtcctcgt taccacctct 841 tggctcacac cacactcacc ctggcagcag tgcaagatgg attccgcaca catgacctca 901 cccttgccag tcatgaggag aaccctgcct ggctgcccct ttatggtagc gtgtgttgcc 961 gtctggcagc tcagcctctc tgcatgactc agcccactgc aagtggtacc ctcagggtgc 1021 agcaagctgg ggagatgcag aactgggcac aagtgcatgg agttctgaaa ggcacaaacc 1081 tcttctgtta ccggcaacct gaggatgcag acactgggga agagccgctg cttactattg 1141 ctgtcaacaa ggagactcga gtccgggcag gggagctgga ccaggctcta ggacggccct 1201 tcaccctaag catcagtaac cagtatgggg atgatgaggt gacacacacc cttcagacag 1261 aaagtcggga agcactgcag agctggatgg aggctctgtg gcagcttttc tttgacatga 1321 gccaatggaa gcagtgctgt gatgaaatca tgaaaattga aactcctgct ccccggaaac 1381 caccccaagc actgGCAAAG CAGGGGTCCT TGTACCATGA GATGGCTATT GAGCCGCTGG 1441 ATGACATCGC AGCGGTGACA GACATCCTGA CCCAGCGGGA GGGCGCAAGG CTGGAGACAC 1501 CCCCACCCTG GCTGGCAATG TTTACAGACC AGCCTGCCCT GCCTAACCCC TGCTCGCCTG 1561 CCTCAGTGGC CCCAGCCCCA GACTGGACCC ACCCCCTGCC CTGGGGGAGA CCCCGAACCT 1621 TTTCCCTGGA TGCTGTCCCC CCAGACCACT CCCCTAGGGC TCGCTCGGTT GCCCCCCTCC 1681 CACCTCAGCG ATCCCCACGG ACCAGAGGCC TCTGCAGCAA AGGCCAACCT CGCACTTGGC 1741 TCCAGTCACC AGTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1801 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1861 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCCATATCA AGTGAAAACG 1921 TATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt