Construct: ORF TRCN0000467116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014710.1_s317c1
- Derived from:
- ccsbBroadEn_06376
- DNA Barcode:
- AGGAAAGTCCTCACAACCTTAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HLA-E (3133)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3133 | HLA-E | major histocompatibility co... | NM_005516.6 | 98.4% | 98.3% | 294T>C;382G>A;1060_1074del |
| 2 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010809.2 | 96.2% | 96.1% | (many diffs) |
| 3 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010807.1 | 87.2% | 82.2% | (many diffs) |
| 4 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010808.1 | 87.2% | 82.2% | (many diffs) |
| 5 | human | 3105 | HLA-A | major histocompatibility co... | NM_002116.8 | 84.4% | 76.1% | (many diffs) |
| 6 | human | 3106 | HLA-B | major histocompatibility co... | NM_005514.8 | 84.2% | 75.4% | (many diffs) |
| 7 | human | 3105 | HLA-A | major histocompatibility co... | NM_001242758.1 | 83.7% | 74.5% | (many diffs) |
| 8 | human | 3107 | HLA-C | major histocompatibility co... | NM_001243042.1 | 83.4% | 73.7% | (many diffs) |
| 9 | human | 3107 | HLA-C | major histocompatibility co... | NM_002117.6 | 83.3% | 73.7% | (many diffs) |
| 10 | human | 3135 | HLA-G | major histocompatibility co... | NM_002127.5 | 82% | 71.9% | (many diffs) |
| 11 | human | 3135 | HLA-G | major histocompatibility co... | XM_024446420.1 | 82% | 71.9% | (many diffs) |
| 12 | human | 3135 | HLA-G | major histocompatibility co... | NM_001363567.1 | 80.8% | 70.9% | (many diffs) |
| 13 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010817.1 | 60.6% | 53% | (many diffs) |
| 14 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010818.1 | 60.6% | 53% | (many diffs) |
| 15 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010815.1 | 54.9% | 48.3% | (many diffs) |
| 16 | human | 3136 | HLA-H | major histocompatibility co... | NR_001434.4 | 54.5% | (many diffs) | |
| 17 | human | 3134 | HLA-F | major histocompatibility co... | XM_017010814.1 | 45.4% | 38.8% | (many diffs) |
| 18 | human | 3139 | HLA-L | major histocompatibility co... | NR_027822.1 | 15.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1125
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt agatggaacc ctccttttac tcctctcgga ggccctggcc cttacccaga 121 cctgggcggg ctcccactcc ttgaagtatt tccacacttc cgtgtcccgg cccggccgcg 181 gggagccccg cttcatctct gtgggctacg tggacgacac ccagttcgtg cgcttcgaca 241 acgacgccgc gagtccgagg atggtgccgc gggcgccgtg gatggagcag gaggggtcag 301 agtattggga ccgggagaca cggagcgcca gggacaccgc acagattttc cgagtgaacc 361 tgcggacgct gcgcggctac tacaatcaga gcgaggccgg gtctcacacc ctgcagtgga 421 tgcatggctg cgagctgggg cccgacaggc gcttcctccg cgggtatgaa cagttcgcct 481 acgacggcaa ggattatctc accctgaatg aggacctgcg ctcctggacc gcggtggaca 541 cggcggctca gatctccgag caaaagtcaa atgatgcctc tgaggcggag caccagagag 601 cctacctgga agacacatgc gtggagtggc tccacaaata cctggagaag gggaaggaga 661 cgctgcttca cctggagccc ccaaagacac acgtgactca ccaccccatc tctgaccatg 721 aggccaccct gaggtgctgg gccctgggct tctaccctgc ggagatcaca ctgacctggc 781 agcaggatgg GGAGGGCCAT ACCCAGGACA CGGAGCTCGT GGAGACCAGG CCTGCAGGGG 841 ATGGAACCTT CCAGAAGTGG GCAGCTGTGG TGGTGCCTTC TGGAGAGGAG CAGAGATACA 901 CGTGCCATGT GCAGCATGAG GGGCTACCCG AGCCCGTCAC CCTGAGATGG AAGCCGGCTT 961 CCCAGCCCAC CATCCCCATC GTGGGCATCA TTGCTGGCCT GGTTCTCCTT GGATCTGTGG 1021 TCTCTGGAGC TGTGGTTGCT GCTGTGATAT GGAGGAAGAA GAGCTCAGGT GGAAAAGGAG 1081 GGAGCTACTC TAAGGCTGAG TGGAGCGACA GTGCCCAGGG GTCTGAGTCT CACAGCTTGT 1141 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAAGGAA AGTCCTCACA ACCTTAAGAA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt