Construct: ORF TRCN0000467119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015928.1_s317c1
- Derived from:
- ccsbBroadEn_00444
- DNA Barcode:
- CCTCTCCCACGGCCTAATGCTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN1 (1731)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1731 | SEPTIN1 | septin 1 | NM_001365977.2 | 98.6% | 98.6% | 1_15del |
| 2 | human | 1731 | SEPTIN1 | septin 1 | NM_052838.6 | 98.6% | 98.6% | 1_15del |
| 3 | human | 1731 | SEPTIN1 | septin 1 | XM_017022991.1 | 74.2% | 65.9% | 1_141del;1157_1245del;1332_1482del |
| 4 | human | 1731 | SEPTIN1 | septin 1 | XR_001751850.1 | 67% | 1_167del;927_997del;1340_1641del | |
| 5 | human | 1731 | SEPTIN1 | septin 1 | XR_002957782.1 | 51.8% | (many diffs) | |
| 6 | mouse | 54204 | Sept1 | septin 1 | NM_017461.2 | 88.4% | 95.6% | (many diffs) |
| 7 | mouse | 54204 | Sept1 | septin 1 | XM_006508030.2 | 81.4% | 88% | (many diffs) |
| 8 | mouse | 54204 | Sept1 | septin 1 | XM_006508031.3 | 81.4% | 88% | (many diffs) |
| 9 | mouse | 54204 | Sept1 | septin 1 | XM_006508029.3 | 73.3% | 79.2% | (many diffs) |
| 10 | mouse | 54204 | Sept1 | septin 1 | XM_011241862.1 | 73.3% | 79.2% | (many diffs) |
| 11 | mouse | 54204 | Sept1 | septin 1 | XM_006508032.1 | 45.8% | 46.8% | (many diffs) |
| 12 | mouse | 54204 | Sept1 | septin 1 | XM_011241863.1 | 44% | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgga caaggagtac gtgggttttg ctgccctccc caaccagctg caccgcaagt 121 ctgtcaagaa ggggtttgac ttcacgctaa tggtggcagg ggagtcaggc ctagggaaat 181 ccaccctcat caacagcctc ttcctcacca acctctatga ggatcgccag gtgccagagg 241 ccagtgctcg cttgacacag accctggcca ttgagcgccg gggcgtagag attgaggaag 301 ggggtgtgaa agtgaagctg acccttgtgg acacacctgg ctttggggac tcagtggact 361 gctctgactg ctggcttccg gtggtgaaat tcatcgagga gcaatttgag cagtacctta 421 gggatgagag tggcctgaac cggaagaaca tccaggactc ccgagtccac tgctgcctct 481 acttcatctc acccttcggc cgggggctcc ggcccctaga tgtggccttc ctccgggcag 541 tacacgagaa agtcaacatc atcccagtca ttggcaaagc ggatgctctg atgccccagg 601 aaacccaggc cctcaagcag aagatccggg atcagttgaa ggaagaggag atccacatct 661 accagttccc cgaatgtgac tctgatgaag atgaagactt caagaggcag gatgcagaga 721 tgaaggaaag catccctttt gcagtcgtgg gatcatgcga ggtggtgagg gatggcggga 781 accggccggt gaggGGACGC CGCTACTCCT GGGGGACCGT GGAGGTGGAG AACCCACATC 841 ACTGCGATTT CCTGAACCTG CGACGGATGC TGGTGCAGAC ACACCTGCAG GACCTGAAAG 901 AGGTGACGCA CGATCTGCTC TACGAGGGCT ACCGGGCCCG CTGCCTACAG AGCCTGGCCC 961 GGCCTGGGGC TCGCGATCGA GCCAGCCGCA GTAAGCTTTC CCGCCAGAGC GCCACAGAGA 1021 TCCCGCTGCC CATGCTGCCT CTGGCGGACA CCGAGAAGCT GATCCGCGAG AAAGACGAAG 1081 AGCTGCGCCG CATGCAAGAG ATGCTGGAGA AGATGCAGGC CCAAATGCAG CAGAGCCAGG 1141 CCCAGGGCGA GCAGTCAGAC GCCCTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1201 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1261 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCTCTCCC 1321 ACGGCCTAAT GCTCAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1381 aagatt