Construct: ORF TRCN0000467128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000019.1_s317c1
- Derived from:
- ccsbBroadEn_10676
- DNA Barcode:
- AAGAATATGTGGTAGTGCTTGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMT (275)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 275 | AMT | aminomethyltransferase | NM_001164711.2 | 82.5% | 81.2% | (many diffs) |
| 2 | human | 275 | AMT | aminomethyltransferase | NM_001164712.2 | 74.8% | 74.8% | 1_291del |
| 3 | human | 275 | AMT | aminomethyltransferase | NM_000481.4 | 71% | 69.9% | (many diffs) |
| 4 | human | 275 | AMT | aminomethyltransferase | NM_001164710.2 | 70.7% | 59% | (many diffs) |
| 5 | human | 275 | AMT | aminomethyltransferase | NR_028435.2 | 43.3% | (many diffs) | |
| 6 | mouse | 434437 | Amt | aminomethyltransferase | NM_001013814.1 | 60.4% | 62.7% | (many diffs) |
| 7 | mouse | 434437 | Amt | aminomethyltransferase | XM_011242894.2 | 51.5% | 53.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 933
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtctagtg gttggagaca ttgcagagct aagaccaaac caggggacac 121 tgtcgctgtt taccaacgag gctggaggca tcttagatga cttgattgta accaatactt 181 ctgagggcca cctgtatgtg gtgtccaacg ctggctgctg ggagaaagat ttggccctca 241 tgcaggacaa ggtcagggag cttcagaacc agggcagaga tgtgggcctg gaggtgttgg 301 ataatgccct gctagctctg caaggcccca ctgcagccca ggtactacag gccggcgtgg 361 cagatgacct gaggaaactg cccttcatga ccagtgctgt gatggaggtg tttggcgtgt 421 ctggctgccg cgtgacccgc tgtggctaca caggagagga tggtgtggag atctcggtgc 481 cggtagcggg ggcagttcac ctggcaacag ctattcTGAA AAACCCAGAG GTGAAGCTGG 541 CAGGGCTGGC AGCCAGGGAC AGCCTGCGCC TGGAGGCAGG CCTCTGCCTG TATGGGAATG 601 ACATTGATGA ACACACTACA CCTGTGGAGG GCAGCCTCAG TTGGACACTG GGGAAGCGCC 661 GCCGAGCTGC TATGGACTTC CCTGGAGCCA AGGTCATTGT TCCCCAGCTG AAGGGCAGGG 721 TGCAGCGGAG GCGTGTGGGG TTGATGTGTG AGGGGGCCCC CATGCGGGCA CACAGTCCCA 781 TCCTGAACAT GGAGGGTACC AAGATTGGTA CTGTGACTAG TGGCTGCCCC TCCCCCTCTC 841 TGAAGAAGAA TGTGGCGATG GGTTATGTGC CCTGCGAGTA CAGTCGTCCA GGGACAATGC 901 TGCTGGTAGA GCTTCCTTCA GGACCCTGCT TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 961 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1021 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1081 GAATATGTGG TAGTGCTTGA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1141 ttgtgaaaga tt