Construct: ORF TRCN0000467132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010774.1_s317c1
- Derived from:
- ccsbBroadEn_14045
- DNA Barcode:
- TCTCCGCAAGCTAGCCTTGTTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PGRMC2 (10424)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10424 | PGRMC2 | progesterone receptor membr... | NM_006320.6 | 100% | 100% | |
| 2 | human | 10424 | PGRMC2 | progesterone receptor membr... | XM_011531533.2 | 68.8% | 55.3% | (many diffs) |
| 3 | mouse | 70804 | Pgrmc2 | progesterone receptor membr... | NM_027558.1 | 86.1% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctggtgat ggggacgtga agctaggcac cctggggagt ggcagcgaga 121 gcagcaacga cggcggcagc gagagtccag gcgacgcggg agcggcagcg gaagggggag 181 gctgggcggc ggcggcgttg gcgcttctga cggggggcgg ggaaatgctg ctgaacgtgg 241 cgctggtggc tctggtgctg ctgggggcct accggctgtg ggtgcgctgg gggcggcggg 301 gtctgggggc cggggccggg gCGGGCGAGG AGAGCCCCGC CACCTCTCTG CCTCGCATGA 361 AGAAGCGGGA CTTCAGCTTG GAGCAGCTGC GCCAGTACGA CGGCTCCCGC AACCCGCGCA 421 TCCTGCTCGC GGTCAATGGG AAAGTCTTCG ACGTGACCAA AGGCAGCAAG TTCTACGGCC 481 CGGCGGGTCC ATATGGAATA TTTGCTGGTA GGGATGCCTC CAGAGGACTG GCCACATTTT 541 GCCTAGATAA AGATGCACTT AGAGATGAAT ATGATGATCT CTCAGATTTG AATGCAGTAC 601 AAATGGAGAG TGTTCGAGAA TGGGAAATGC AGTTTAAAGA AAAATATGAT TATGTAGGCA 661 GACTCCTAAA ACCAGGAGAA GAACCATCAG AATATACAGA TGAAGAAGAT ACCAAGGATC 721 ACAATAAACA GGATTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCTCCGCAAG CTAGCCTTGT 901 TAAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt