Construct: ORF TRCN0000467135
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011435.1_s317c1
- Derived from:
- ccsbBroadEn_16071
- DNA Barcode:
- GCCGCTTACCTCTTTAAGGCTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPA5 (93979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467135
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93979 | CPA5 | carboxypeptidase A5 | NM_001127441.2 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 93979 | CPA5 | carboxypeptidase A5 | NM_001318223.2 | 99.6% | 99.5% | (many diffs) |
| 3 | human | 93979 | CPA5 | carboxypeptidase A5 | NM_080385.5 | 99.6% | 99.5% | (many diffs) |
| 4 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_005250710.1 | 99.6% | 99.5% | (many diffs) |
| 5 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_005250712.1 | 99.6% | 99.5% | (many diffs) |
| 6 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516698.1 | 99.6% | 99.5% | (many diffs) |
| 7 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516701.1 | 99.6% | 99.5% | (many diffs) |
| 8 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516702.1 | 99.6% | 99.5% | (many diffs) |
| 9 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516703.1 | 99.6% | 99.5% | (many diffs) |
| 10 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_024446999.1 | 99.6% | 99.5% | (many diffs) |
| 11 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_024447000.1 | 99.6% | 99.5% | (many diffs) |
| 12 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_024447001.1 | 99.6% | 99.5% | (many diffs) |
| 13 | human | 93979 | CPA5 | carboxypeptidase A5 | NM_001127442.2 | 92.1% | 80.1% | (many diffs) |
| 14 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516704.1 | 92.1% | 80.1% | (many diffs) |
| 15 | human | 93979 | CPA5 | carboxypeptidase A5 | XM_011516705.1 | 67.1% | 64.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gggcactcct ggaggcggga cgcgccctgg gccatccccc gtggacaggc 121 ggacactcct ggtcttcagc tttatcctgg cagcagcttt gggccaaatg aatttcacag 181 gggaccaggt tcttcgagtc ctggccaaag atgagaagca gctttcactt ctcggggatc 241 tggagggcct gaaaccccag aaggtggact tctggcgtgg cccagccagg cccagcctcc 301 ctgtggatat gagagttcct ttctctgaac tgaaagacat caaagcttat ctggagtctc 361 atggacttgc ttacagcatc atgataaagg acatccaggt gctgctggat gaggaaagac 421 aggccatggc gaaatcccgc cggctggagc gcagcaccaa cagcttcagt tactcatcat 481 accacaccct ggaggagata tatagctgga ttgacaactt tgtaatggag cattccgata 541 ttgtctcaaa aattcagatt ggcaacagct ttgaaaacca gtccattctt gtcctgaagt 601 tcagcactgg aggttctcgg cacccagcca tctggattga cactggaatt cactcccggg 661 agtggatcac ccatgccacc ggcatctgga ctgccaataa gattgtcagt gattatggca 721 aagaccgtgt cctgacagac atactgaatg ccatggacat cttcatagag ctcgtcacaa 781 accctgatgg gtttgctttt acccacagca tgaaccgctt atggcggaag aacaagtcca 841 tcagacctgg aatcttctgc atcggcgtgg atctcaacag gaactggaag tcgggttttg 901 gaggaaatgg ttctaacagc aacccctgct cagaaactta tcacgggccc tccccTCAGT 961 CGGAGCCGGA GGTGGCTGCC ATAGTGAACT TCATCACAGC CCATGGCAAC TTCAAGGCTC 1021 TGATCTCCAT CCACAGCTAC TCTCAGATGC TTATGTACCC TTACGGCCGA TCGCTGGATC 1081 CCGTTTCAAA TCAGAGGGAG TTGTACGATC TTGCCAAGGA TGCGGTGGAG GCCTTGTATA 1141 AGGTCCATGG GATCGAGTAC ATTTTTGGCA GCATCAGCAC CACCCTCTAT GTGGCCAGTG 1201 GGATCACCGT CGACTGGGCC TACGACAGTG GCATCAAGTA CGCCTTCAGC TTTGAGCTCC 1261 GGGACACTGG GCAGTATGGC TTCCTGCTGC CGGCCACACA GATCATCCCC ACGGCCCAGG 1321 AGACGTGGAT GGCGCTTCGG ACCATCATGG AGCACACCCT GAATCACCCC TACTACCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAG CCGCTTACCT CTTTAAGGCT CTAACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att