Transcript: Human XM_011516705.1

PREDICTED: Homo sapiens carboxypeptidase A5 (CPA5), transcript variant X11, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CPA5 (93979)
Length:
2670
CDS:
1200..2126

Additional Resources:

NCBI RefSeq record:
XM_011516705.1
NBCI Gene record:
CPA5 (93979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516705.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047043 GCCAATAAGATTGTCAGTGAT pLKO.1 1827 CDS 100% 4.950 6.930 N CPA5 n/a
2 TRCN0000047046 CGGAAGAACAAGTCCATCAGA pLKO.1 1959 CDS 100% 3.000 4.200 N CPA5 n/a
3 TRCN0000371833 CCCACAGCATGAACCGCTTAT pLKO_005 1936 CDS 100% 10.800 7.560 N CPA5 n/a
4 TRCN0000047044 GCATTCCGATATTGTCTCAAA pLKO.1 1664 CDS 100% 4.950 3.465 N CPA5 n/a
5 TRCN0000047047 CTGTGGATATGAGAGTTCCTT pLKO.1 1435 CDS 100% 3.000 2.100 N CPA5 n/a
6 TRCN0000371890 AGCTTCAGTTACTCATCATAC pLKO_005 1596 CDS 100% 10.800 6.480 N CPA5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516705.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_16071 pDONR223 0% 67.1% 64.9% None (many diffs) n/a
2 ccsbBroad304_16071 pLX_304 0% 67.1% 64.9% V5 (many diffs) n/a
3 TRCN0000467135 GCCGCTTACCTCTTTAAGGCTCTA pLX_317 32.3% 67.1% 64.9% V5 (many diffs) n/a
4 ccsbBroadEn_09363 pDONR223 100% 66.9% 64.7% None (many diffs) n/a
5 ccsbBroad304_09363 pLX_304 0% 66.9% 64.7% V5 (many diffs) n/a
6 TRCN0000469314 TGTTTTCCGGTAATCCACTCCGCC pLX_317 32.3% 66.9% 64.7% V5 (many diffs) n/a
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