Construct: ORF TRCN0000467138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013228.1_s317c1
- Derived from:
- ccsbBroadEn_00521
- DNA Barcode:
- TTCGAGCCTGTGGTCAGAATCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETV5 (2119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467138
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2119 | ETV5 | ETS variant transcription f... | NM_004454.3 | 100% | 100% | |
| 2 | mouse | 104156 | Etv5 | ets variant 5 | NM_023794.2 | 90% | 95.4% | (many diffs) |
| 3 | mouse | 104156 | Etv5 | ets variant 5 | XM_006521657.3 | 90% | 95.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1596
- ORF length:
- 1530
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgggttttat gatcagcaag tcccttttat ggtcccaggg aaatctcgat 121 ctgaggaatg cagagggcgg cctgtgattg acagaaagag gaagtttttg gacacagatc 181 tggctcacga ttctgaagag ctatttcagg atctcagtca acttcaagag gcttggttag 241 ctgaagcaca agttcctgat gatgaacagt ttgtcccaga ttttcagtct gataacctgg 301 tgcttcatgc cccacctcca accaagatca aacgggagct gcacagcccc tcctctgagc 361 tgtcgtcttg tagccatgag caggctcttg gtgctaacta tggagaaaag tgcctctaca 421 actattgtgc ctatgatagg aagcctccct ctgggttcaa gccattaacc cctcctacaa 481 cccccctctc acccacccat cagaatcccc tatttccccc acctcaggca actctgccca 541 cctcagggca tgcccctgca gctggcccag ttcaaggtgt gggccccgcc cccgcccccc 601 attcgcttcc agagcctgga ccacagcagc aaacatttgc ggtcccccga ccaccacatc 661 agcccctgca gatgccaaag atgatgcctg aaaaccagta tccatcagaa cagagatttc 721 agagacaact gtctgaaccc tgccacccct tccctcctca gccaggagtt cctggagata 781 atcgccccag ttaccatcgg caaatgtcag aacctattgt ccctgcagct cccccgcccc 841 ctcagggatt caaacaagaa taccatgacc cactctatga acatggggtc ccgggcatgc 901 cagggccccc agcacacggg ttccagtcac caatgggaat caagcaggag cctcgggatt 961 actgcgtcga ttcagaagtg cctaactgcc agtcatccta catgagaggg ggttatttct 1021 ccagcagcca tgaaggtttt tcatatgaaa aagatccccg attatacttt gacgacactt 1081 gtgttgtgcc tgagagactg gaaggcaaag tcaaacagga gcctaccatg tatcgagagg 1141 ggccccctta ccagaggcga ggttcccttc agctgtggca gttcctggTC ACCCTTCTTG 1201 ATGACCCAGC CAATGCCCAC TTCATTGCCT GGACAGGTCG AGGCATGGAG TTCAAGCTGA 1261 TAGAACCGGA AGAGGTTGCT CGGCGCTGGG GCATCCAGAA GAACCGGCCA GCCATGAACT 1321 ATGACAAGCT GAGCCGCTCT CTCCGCTATT ACTATGAAAA GGGCATCATG CAGAAGGTGG 1381 CTGGAGAGCG ATACGTCTAC AAATTTGTCT GTGACCCAGA TGCCCTCTTC TCCATGGCTT 1441 TCCCGGATAA CCAGCGTCCG TTCCTGAAGG CAGAGTCCGA GTGCCACCTC AGCGAGGAGG 1501 ACACCCTGCC GCTGACCCAC TTTGAAGACA GCCCCGCTTA CCTCCTGGAC ATGGACCGCT 1561 GCAGCAGCCT CCCCTATGCC GAAGGCTTTG CTTACTACCC AACTTTCTTG TACAAAGTGG 1621 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1681 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1741 ATTCGAGCCT GTGGTCAGAA TCACTACGCG TTAAGTCgac aatcaacctc tggattacaa 1801 aatttgtgaa agatt