Construct: ORF TRCN0000467146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018953.2_s317c1
- Derived from:
- ccsbBroadEn_14695
- DNA Barcode:
- TCTTGTTAAGTTATAACTTGTTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K5 (4217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467146
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4217 | MAP3K5 | mitogen-activated protein k... | NM_005923.4 | 93.4% | 3% | (many diffs) |
| 2 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010873.1 | 86.8% | 2.8% | (many diffs) |
| 3 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010872.1 | 86.8% | 2.8% | (many diffs) |
| 4 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010871.1 | 85.6% | 2.7% | (many diffs) |
| 5 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010870.1 | 85.5% | 2.7% | (many diffs) |
| 6 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010875.1 | 84.5% | 2.8% | (many diffs) |
| 7 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010874.1 | 83.2% | 2.8% | (many diffs) |
| 8 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010877.1 | 82.8% | 1.1% | (many diffs) |
| 9 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_017010876.1 | 81.4% | 1.1% | (many diffs) |
| 10 | human | 4217 | MAP3K5 | mitogen-activated protein k... | XM_011535839.3 | 74% | 3.2% | (many diffs) |
| 11 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | NM_008580.4 | 81.7% | 2.5% | (many diffs) |
| 12 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XM_006512739.3 | 61.2% | 3.2% | (many diffs) |
| 13 | mouse | 26408 | Map3k5 | mitogen-activated protein k... | XR_001779529.1 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 267
- ORF length:
- 198
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcacggag gcggacgagg gcatcacttt ctctgtgcca cccttcgccc 121 cctcgggctt ctgcaccatc cccgagggcg gcatctgcag gaggggagga gcggcggcgg 181 tgtgtttttt tcactattca aacaggccat cgtcgcctcc ccggcaccgt tcactctcaa 241 ccagtcggtc cgttcatact cttcagtaaa tgtgccataa gaatttaaat gaaatacatg 301 caattcagga atcttcttgg gtagggaatt tagcttcacg tggataatat cttggtcttt 361 ttcgtaaccc actagatgaa tgagtgctgg taattctttc tctgccccaa ctaattcttc 421 agtactctcg agtcctctag tcacatcatt ctaaactgca cattcagtga catgtgagca 481 ctccggcaat catgagatac actacaactg agcgtgctca tgcctactgg tggtggccga 541 gagcgaggcc ctgcagagct tgcgggaggc gtgcgagaca gtgggcgcca ccctggaaac 601 cctgcatttt gggaaactcg actttggaga aaccaccgtg ctggaccgct tttacaatgc 661 agatattgcg gtggtggaga tgagcgatgc cttccggcag ccgtccttgt tttaccacct 721 tggggtgaga gaaagtttca gcatggccaa caacatcatc ctctactgtg atactaactc 781 ggactctctg cagtcactga aggaaatcat ttgccagaag aatactatgt gcactgggaa 841 ctacaccttt gttccttaca tgataactcc acataacaaa gtctactgct gtgacagcag 901 cttcatgaag gggttgacag agctcatgca accgaacttc gagctgcttc ttggacccat 961 ctgcttacct cttgtggatc gttttattca acttttgaag gtggcacaag caagttctag 1021 ccagtacttc cgggaatcta tactcaatga catcaggaaa gctcgtaatt tatacactgg 1081 taaagaattg gcagctgagt tggcaagaat tcggcagcga gtagataata tcgaagtctt 1141 gacagcagat attgtcataa atctgttact ttcctacaga gatatccagg actatgattc 1201 tattgtgaag ctggtagaga ctttagaaaa actgccaacc tttgatttgg cctcccatca 1261 ccatgtgaag tttcattatg catttgcact gaataggaga aatctccctg gtgacagagc 1321 aaaagctctt gatattatga ttcccatggt gcaaagcgaa ggacaagttg cttcagatat 1381 gtattgccta gttggtcgaa tctacaaaga tatgtttttg gactctaatt tcacggacac 1441 tgaaagcaga gaccatggag cttcttggtt caaaaaggca tttgaatctg agccaacact 1501 acagtcagga attaattatg cggtcctcct cctggcagct ggacaccagt ttgaatcttc 1561 ctttgagctc cggaaagttg gggtgaagct aagtagtctt cttggtaaaa agggaaactt 1621 ggaaaaactc cagagctact gggaagttgg attttttctg ggggccagcg tcctagccaa 1681 tgaccacatg agagtcattc aagcatctga aaagcttttt aaactgaaga caccagcatg 1741 gtacctcaag tctattgtag agacaatttt aatatataag cattttgtga aactgaccac 1801 agaacagcct gtggccaagc aagaacttgt ggacttttgg atggatttcc tggtcgaggc 1861 cacaaagaca gatgttactg tggttaggtt tccagtatta atattagaac caaccaaaat 1921 ctatcaacct tcttatttgt ctatcaacaa tgaagttgag gaaaagacaa tctctatttg 1981 gcacgtgctt cctgatgaca agaaaggtat acatgagtgg aattttagtg cctcttctgt 2041 caggggagtg agtatttcta aatttgaaga aagatgctgc tttctttatg tgcttcacaa 2101 ttctgatgat ttccaaatct atttctgtac agaacttcat tgtaaaaagt tttttgagat 2161 ggtgaacacc attaccgaag agaaggggag aagcacagag gaaggagact gtgaaagtga 2221 cttgctggag tatgactatg aatatgatga aaatggtgac agagtcgttt taggaaaagg 2281 cacttatggg atagtctacg caggtcggga cttgagcaac caagtcagaa ttgctattaa 2341 ggaaatccca gagagagaca gcagatactc tcagcccctg catgaagaaa tagcattgca 2401 taaacacctg aagcacaaaa atattgtcca gtatctgggc tctttcagtg agaatggttt 2461 cattaaaatc ttcatggagc aggtccctgg aggaagtctt tctgctctcc ttcgttccaa 2521 atggggtcca ttaaaagaca atgagcaaac aattggcttt tatacaaagc aaatactgga 2581 aggattaaaa tatctccatg acaatcagat agttcaccgg gacataaagg gtgacaatgt 2641 gttgattaat acctacagtg gtgttctcaa gatctctgac ttcggaacat caaagaggct 2701 tgctggcata aacccctgta ctgaaacttt tactggtacc ctccagtata tggcaccaga 2761 aataatagat aaaggaccaa gaggctacgg aaaagcagca gacatctggt ctctgggctg 2821 tacaatcatt gaaatggcca caggaaaacc cccattttat gaactgggag aaccacaagc 2881 agctatgttc aaggtgggaa tgtttaaagt ccaccctgag atcccagagt ccatgtctgc 2941 agaggccaag gcattcatac tgaaatgttt tgaaccagat cctgacaaga gagcctgtgc 3001 taacgacttg cttgttgatg agtttttaaa agtttcaagc aaaaagaaaa agacacaacc 3061 taagctttca gctctttcag ctggatcaaa tgaatatctc aggagtatat ccttgccggt 3121 acctgtgctg gtggaggaca ccagcagcag cagtgagtac ggctcagttt cacccgacac 3181 ggagttgaaa gtggacccct tctctttcaa aacaagagcc aagtcctgcg gagaaagaga 3241 tgtcaaggga attcggacac tctttttggg cattccagat gagaattttg aagatcacag 3301 tgctcctcct tcccctgaag aaaaagattc tggattcttc atgctgagga aggacagtga 3361 gaggcgagct acccttcaca ggatcctgac ggaagaccaa gacaaaattg tgagaaacct 3421 aatggaatct ttagctcagg gggctgaaga accgaaacta aaatgggaac acatcacaac 3481 cctcattgca agcctcagag aatttgtgag atccactgac cgaaaaatca tagccaccac 3541 actgtcaaag ctgaaactgg agctggactt cgacagccat ggcattagcc aagtccaggt 3601 ggtactcttt ggttttcaag atgctgtcaa taaagttctt cggaatcata acatcaagcc 3661 gcactggatg tttgccttag acagtatcat tcggaaggcg gtacagacag ccattaccat 3721 cctggttcca gaactaaggc cacatttcag ccttgcatct gagagtgata ctgctgatca 3781 agaagacttg gatgtagaag atgaccatga ggaacagcct tcaaatcaaa ctgtccgaag 3841 acctcaggct gtcattgaag atgctgtggc tacctcaggc gtgagcacgc tcagttctac 3901 tgtgtctcat gattcccaga gtgctcaccg gtcactgaat gtacagcttg gaaggatgaa 3961 aatagaaacc aatagattac tggaagaatt ggttcGGAAA GAGAAAGAAT TACAAGCACT 4021 CCTTCATCGA GCTATTGAAG AAAAAGACCA AGAAATTAAA CACCTGAAGC TTAAGTCCCA 4081 ACCCATAGAA ATTCCTGAAT TGCCTGTATT TCATCTAAAT TCTTCTGGCA CAAATACTGA 4141 AGATTCTGAA CTTACCGACT GGCTGAGAGT GAATGGAGCT GATGAAGACA CTATAAGCCG 4201 GTTTTTGGCT GAAGATTATA CACTATTGGA TGTTCTCTAC TATGTTACAC GTGATGACTT 4261 AAAATGCTTG AGACTAAGGG GAGGGATGCT GTGCACACTG TGGAAGGCTA TCATTGACTT 4321 TCGAAACAAA CAGACTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 4381 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 4441 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTTGTTA AGTTATAACT 4501 TGTTTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt