Construct: ORF TRCN0000467148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005686.1_s317c1
- Derived from:
- ccsbBroadEn_13147
- DNA Barcode:
- TTCCATGCTATTCTTCTTCCACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF8 (129685)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129685 | TAF8 | TATA-box binding protein as... | NM_138572.3 | 98.1% | 98.7% | 922_928delTCCCTCT;930_931insAGATACATTC |
| 2 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010241.2 | 96.7% | 95.9% | (many diffs) |
| 3 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_011514296.2 | 84.5% | 79.4% | 780_901del;1014_1015ins41 |
| 4 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010243.2 | 78% | 78.4% | 0_1ins189;733_739delTCCCTCT;741_742insAGATACATTC |
| 5 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010242.2 | 77% | 76.1% | (many diffs) |
| 6 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010245.1 | 67.2% | 67.5% | 488_489ins291;631_637delTCCCTCT;639_640insAGATACATTC |
| 7 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010244.2 | 66.4% | 65.5% | (many diffs) |
| 8 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_005248849.2 | 52.4% | 52.4% | 489_490ins444 |
| 9 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524765.3 | 88.2% | 93.2% | (many diffs) |
| 10 | mouse | 63856 | Taf8 | TATA-box binding protein as... | NM_022015.3 | 88.1% | 93.2% | (many diffs) |
| 11 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524766.3 | 79.8% | 80.4% | (many diffs) |
| 12 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524764.2 | 77.9% | 76.1% | (many diffs) |
| 13 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524767.3 | 76.4% | 80.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1002
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgacgcg gcggccacag ctggggccgg tggctccgga acgagatcgg 121 gaagtaaaca gtccactaac cctgccgata actatcatct ggcccggagg agaaccctgc 181 aggtggttgt gagctccttg ctgacagagg cagggtttga gagtgccgag aaagcatccg 241 tggaaacgct gacagagatg ctgcagagct acatttcaga aattgggaga agtgccaagt 301 cttactgtga gcacacagcc aggacccagc ccacactgtc cgatatcgtg gtcacacttg 361 ttgagatggg tttcaatgtg gacactctcc ctgcttatgc aaaacggtct cagaggatgg 421 tcatcactgc tcctccggtg accaatcagc cagtgacccc caaggccctc actgcagggc 481 agaaccgacc ccacccgccg cacatcccca gccattttcc tgagttccct gatccccaca 541 cctacatcaa aactccgacg taccgtgagc ccgtgtcaga ctaccaggtc ctgcgggaga 601 aggctgcatc ccagaggcgc gatgtggagc gggcacttac ccgtttcatg gccaagacag 661 gcgagactca gagtcttttc aaagatgacg tcagcacatt tccattgatt gctgccagac 721 ctttcaccat cccctacctg acagctcttc ttccgtctga actggagatg caacaaatgg 781 aagagacaga ttcctcggag caggatgaac agacagacac agagaacctt gctcttcata 841 tcagcatgga ggattctGGA GCCGAGAAGG AGAACACCTC TGTCCTGCAG CAGAACCCCT 901 CCTTGTCGGG TAGCCGGAAT GGGGAGGAGA ACATCATCGA TAACCCTTAT CTGCGGCCGG 961 TGAAGAAGCC CAAGATCCGC AGGAAGAAGC CAGATACATT CTTGCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGATTC CATGCTATTC TTCTTCCACA TACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t