Construct: ORF TRCN0000467151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018222.1_s317c1
- Derived from:
- ccsbBroadEn_09499
- DNA Barcode:
- GCTGGCTCTAACTAGCCCCAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC25 (126364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467151
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126364 | LRRC25 | leucine rich repeat contain... | NM_145256.3 | 99.8% | 99.6% | 880C>T |
| 2 | human | 126364 | LRRC25 | leucine rich repeat contain... | XM_005259739.4 | 99.8% | 99.6% | 880C>T |
| 3 | human | 126364 | LRRC25 | leucine rich repeat contain... | XR_001753602.2 | 43.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gggcaccctg gcatggacgc tgctgttgcc gctgctgctg cgggagtcag 121 acagcctaga accgtcgtgc accgtgtcct ccgcggatgt ggactggaac gcggagttca 181 gtgccacgtg cctgaatttc agtggcctca gcctgagcct gcctcacaac cagtctctgc 241 gggccagcaa cgtgattctc cttgacctgt ctgggaacgg cctgcgagag cttccagtga 301 ccttctttgc ccacctgcag aagcttgagg tcctgaacgt gctacgcaac cccttgtctc 361 gtgtggatgg ggcgctggcc gcccgctgtg accttgacct gcaggccgac tgcaactgtg 421 ccctggagtc ctggcacgac atccgccgag acaactgctc tggccagaag cctctgctct 481 gctgggacac aaccagctcc cagcacaacc tctctgcctt cctggaggtc agctgcgccc 541 ctggcctggc ctctgcaact atcggggcag tggtggtcag cgggtgcctg cttcttggac 601 ttgccatcgc tggccctgtg ctggcctgga gactctggcg atgccgagtg gccagaagcc 661 gggagctgaa caaaccctgg gctgctcagg atgggcccaa gcccggttta ggcttGCAGC 721 CACGGTACGG CAGCCGGAGC GCCCCCAAGC CCCAAGTGGC CGTGCCATCC TGCCCCTCCA 781 CTCCCGACTA TGAGAACATG TTTGTGGGCC AGCCAGCAGC CGAGCACCAG TGGGATGAAC 841 AAGGGGCTCA CCCTTCAGAG GACAATGACT TTTACATCAA CTACAAGGAC ATCGACCTGG 901 CTTCCCAGCC TGTCTACTGT AACCTGCAGT CACTGGGCCA GGCCTCAATG GATGAAGAGG 961 AGTACGTGAT CCCCGGGCAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1021 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1081 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCTG GCTCTAACTA 1141 GCCCCAATCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt