Transcript: Human XM_005259739.4

PREDICTED: Homo sapiens leucine rich repeat containing 25 (LRRC25), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
LRRC25 (126364)
Length:
1967
CDS:
200..1117

Additional Resources:

NCBI RefSeq record:
XM_005259739.4
NBCI Gene record:
LRRC25 (126364)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005259739.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418534 TTGAGGTCCTGAACGTGCTAC pLKO_005 459 CDS 100% 4.050 5.670 N LRRC25 n/a
2 TRCN0000153328 GTACAGGAAAGGATTGTCCTT pLKO.1 1222 3UTR 100% 2.640 2.112 N LRRC25 n/a
3 TRCN0000154539 GTGATTCTCCTTGACCTGTCT pLKO.1 386 CDS 100% 2.640 2.112 N LRRC25 n/a
4 TRCN0000436801 CTCACCCTTCAGAGGACAATG pLKO_005 981 CDS 100% 10.800 7.560 N LRRC25 n/a
5 TRCN0000154781 GAGCTTCCAGTGACCTTCTTT pLKO.1 422 CDS 100% 5.625 3.938 N LRRC25 n/a
6 TRCN0000431911 GTGCCACGTGCCTGAATTTCA pLKO_005 315 CDS 100% 5.625 3.938 N LRRC25 n/a
7 TRCN0000156241 CCCTTCAGAGGACAATGACTT pLKO.1 985 CDS 100% 4.950 3.465 N LRRC25 n/a
8 TRCN0000152897 GAATGGGTACAGGAAAGGATT pLKO.1 1216 3UTR 100% 4.950 3.465 N LRRC25 n/a
9 TRCN0000154140 CATCAACTACAAGGACATCGA pLKO.1 1009 CDS 100% 2.640 1.848 N LRRC25 n/a
10 TRCN0000155959 CTACTGTAACCTGCAGTCACT pLKO.1 1048 CDS 100% 2.640 1.848 N LRRC25 n/a
11 TRCN0000155960 CAGCAACGTGATTCTCCTTGA pLKO.1 379 CDS 100% 4.050 2.430 N LRRC25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005259739.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09499 pDONR223 100% 99.8% 99.6% None 880C>T n/a
2 ccsbBroad304_09499 pLX_304 0% 99.8% 99.6% V5 880C>T n/a
3 TRCN0000467151 GCTGGCTCTAACTAGCCCCAATCA pLX_317 29.5% 99.8% 99.6% V5 880C>T n/a
4 ccsbBroadEn_13116 pDONR223 100% 32.7% 7.8% None 1_301del;602_915del n/a
5 ccsbBroad304_13116 pLX_304 0% 32.7% 7.8% V5 1_301del;602_915del n/a
6 TRCN0000479448 CCGGAATGAGGCCGAGCTCCCCCA pLX_317 100% 32.7% 7.8% V5 1_301del;602_915del n/a
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