Construct: ORF TRCN0000467195

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013270.1_s317c1
Derived from:
ccsbBroadEn_02765
DNA Barcode:
CCGACTGTACATGTTCGCCGCGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AP4E1 (23431)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467195
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23431 AP4E1 adaptor related protein com... NM_007347.5 100% 100%
2 human 23431 AP4E1 adaptor related protein com... NM_001252127.2 93.4% 93.4% 0_1ins225
3 human 23431 AP4E1 adaptor related protein com... XM_005254264.4 93.4% 93.4% 0_1ins225
4 human 23431 AP4E1 adaptor related protein com... XM_006720447.4 93.4% 93.4% 0_1ins225
5 human 23431 AP4E1 adaptor related protein com... XR_001751184.1 76.5% 1_107del;2073_2074ins124;3395_4168del
6 human 23431 AP4E1 adaptor related protein com... XM_017022042.2 74.1% 74.1% 0_1ins882
7 human 23431 AP4E1 adaptor related protein com... XR_001751185.1 52.5% 1_107del;1424_1425delAC;1960_1961ins1560
8 human 23431 AP4E1 adaptor related protein com... XR_001751183.1 47.6% 1_107del;3009_3010ins191;3328_6568del
9 mouse 108011 Ap4e1 adaptor-related protein com... XM_006498553.2 47.9% 49.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3480
ORF length:
3411
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagcgacata gtggagaaga cgctgacggc gctgccggga ctctttctgc
121agaaccagcc cggtggtggg cccgcggccg ccaaggcgtc cttctcctcg aggctgggca
181gccttgtccg cggcatcaca gccctcacct ccaagcacga agaagaaaaa ttaatccagc
241aggaactgag tagtctgaaa gcgactgttt ctgctcctac tacaacactg aaaatgatga
301aggaatgtat ggtgagactt atatattgtg aaatgcttgg atatgatgct tcctttggct
361atattcatgc aatcaagtta gcccaacaag gaaacctctt agaaaaaaga gtaggttatt
421tggctgtttc cttatttcta catgaaagtc atgaattatt gcttctcctt gtgaatacag
481ttgtaaagga tctgcagagc actaacctag tagaagtgtg tatggcactg actgttgtta
541gccagatttt cccctgcgaa atgattccag ctgttcttcc attaatagaa gataaacttc
601aacattctaa ggagattgta cgaagaaaag ctgttctggc attatacaaa ttccatctca
661ttgctcctaa tcaagtacaa catattcata ttaagtttcg gaaagcactt tgtgacagag
721atgttggggt catggctgcc tccttgcata tatatcttag aatgattaag gagaattcat
781ctggatataa agacttgact gggagttttg taaccatttt gaagcaagta gttggaggaa
841agctcccagt agaattcaat taccacagtg tgccagcacc atggttacaa attcagctct
901tgagaatact gggacttcta ggaaaagatg atcaaaggac aagtgaatta atgtatgatg
961ttcttgatga atccttacga agagctgagt taaatcacaa tgtcacatat gctattttgt
1021ttgaatgtgt gcatacagtc tattctattt atcctaaatc ggaattactt gagaaggctg
1081ccaagtgcat tggaaaattt gttctgtcac ctaaaataaa tctaaaatat ttaggactga
1141aggctcttac ctatgttatc caacaggatc ctactctggc tcttcaacac cagatgacaa
1201taattgaatg tttagatcat cctgatccca ttattaaaag agagactctg gaacttcttt
1261acagaattac taatgcacag aatataacag ttattgtcca gaaaatgctt gaatatttac
1321atcagagcaa agaagagtat gtcatcgtca atttggtcgg caaaatagca gagctggctg
1381agaaatatgc tcctgataat gcatggttta ttcagacaat gaatgctgtg ttttcagtag
1441gaggagatgt aatgcatcct gatattccca ataactttct gagactacta gcggaaggtt
1501ttgatgatga aacagaagat cagcaattaa gactctatgc agttcagtct tatctcactt
1561tactggatat ggaaaatgtg ttctatccac agagatttct tcaagttatg agttgggtat
1621taggggaata ttcctacctc ttagataagg aaacgccaga ggaagttata gctaagctct
1681acaagttact tatgaatgac tctgtgtctt cagaaacaaa agcctggtta attgctgctg
1741tgaccaaatt gacatctcag gcgcactctt ctaatacagt tgagagatta atccatgaat
1801ttaccatatc tttggatact tgtatgagac aacatgcatt tgaattaaaa catttgcatg
1861agaatgtgga acttatgaag agcttgcttc cagttgacag gagttgtgaa gacttggtgg
1921tagatgcttc tttatctttt ctggatggtt ttgtggctga aggactcagt cagggtgcag
1981cgccttacaa acctccccat caacgccagg aggaaaagct ttctcaggaa aaagttctca
2041attttgaacc atatggactc tccttttctt catctggctt cactggacga cagtctcctg
2101ctggcatttc tcttggttca gatgtatctg ggaatagtgc tgagacagga ctgaaagaga
2161caaatagctt gaagctggaa ggtataaaga aattgtgggg gaaagaaggc tatcttccca
2221agaaggaaag caaaactggt gatgaaagtg gagctctgcc tgttcctcaa gagagtataa
2281tggagaatgt agatcaagct ataactaaaa aggatcaatc tcaagttctt acccaatcta
2341aagaggagaa agaaaagcag ctgctggcat catcattatt tgttggtcta ggatcagaaa
2401gtacaatcaa cctgctggga aaagcagata ctgtctctca caagttcaga aggaaatcaa
2461aagtcaaaga agctaaaagt ggcgaaacaa ccagtactca taatatgacc tgttcttcct
2521ttagttcttt gtcaaatgtg gcatatgaag atgattatta ttcgaatact ttgcacgata
2581caggagacaa ggaattaaag aaattttctc tcacttcaga acttttggat tctgagtcac
2641tcacagaact gcccttggtt gagaaattct catattgtag tctgtctaca ccttcattgt
2701ttgctaataa caacatggaa atttttcacc ctcctcaatc tactgcagcc tcagttgcca
2761aggaaagctc tttagcttca tcttttttgg aagaaactac tgaatacata cactcaaatg
2821ctatggaagt ctgtaataat gaaactatat cagtgtcttc ttataaaatt tggaaagatg
2881attgtttatt gatggtctgg tcagtcacta ataagagtgg tttggaattg aaaagtgctg
2941acttagaaat ttttcctgca gaaaatttca aggtgactga gcaacctgga tgctgtttgc
3001ctgtaatgga agcagaaagc accaaaagct ttcaatatag tgtgcagata gaaaaacctt
3061ttacagaagg aaatcttact ggttttatta gttatcatat gatggatact cattctgcTC
3121AGCTGGAATT TTCTGTAAAC TTATCACTAT TAGATTTCAT TAGACCATTA AAAATCTCAA
3181GTGACGACTT TGGGAAACTC TGGTTATCCT TCGCAAATGA TGTGAAACAA AATGTAAAAA
3241TGTCAGAATC TCAAGCTGCA CTTCCTTCTG CACTAAAGAC TCTGCAACAG AAACTAAGAC
3301TCCATATTAT TGAGATTATA GGCAATGAAG GGCTATTGGC CTGTCAGCTG CTCCCATCCA
3361TCCCCTGCTT ACTGCATTGC CGAGTTCATG CAGATGTATT AGCCCTGTGG TTCAGATCCT
3421CCTGTTCTAC TCTTCCTGAC TATTTACTGT ATCAGTGTCA AAAGGTGATG GAGGGATCCT
3481TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
3541GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
3601TTTATATATC TTGTGGAAAG GACGACCGAC TGTACATGTT CGCCGCGTGA CGCGTTAAGT
3661Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

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