Construct: ORF TRCN0000467196

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005389.1_s317c1
Derived from:
ccsbBroadEn_12409
DNA Barcode:
AACCAAGCCCCCATACGACCTATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC4A5 (57835)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467196
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57835 SLC4A5 solute carrier family 4 mem... NM_133478.3 90.9% 90.8% 80_271del;2316_2429del
2 human 57835 SLC4A5 solute carrier family 4 mem... NM_021196.3 89.6% 89.5% 80_271del;2316_2429del;2917_2964del
3 mouse 232156 Slc4a5 solute carrier family 4, so... NM_001166067.1 80.6% 84.6% (many diffs)
4 mouse 232156 Slc4a5 solute carrier family 4, so... XM_006505967.2 79.4% 82.8% (many diffs)
5 mouse 232156 Slc4a5 solute carrier family 4, so... XM_006505965.2 77.5% 80.8% (many diffs)
6 mouse 232156 Slc4a5 solute carrier family 4, so... XM_006505964.2 75.2% 78.4% (many diffs)
7 mouse 232156 Slc4a5 solute carrier family 4, so... XM_006505963.3 70.5% 73.5% (many diffs)
8 mouse 232156 Slc4a5 solute carrier family 4, so... XM_006505966.2 70.3% 73.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3126
ORF length:
3057
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaaggtgaag gaggagaagg ctggggtagg aaagctggac cacactaacc
121acaggaggag atttccggat cagaaagggt ccccagctgc tgagcagctc caggacatcc
181tgggggagga agatgaggct cccaacccca ccctctttac agagatggat actctgcagc
241atgacggaga ccagatggag tggaaggagt cagccaggtg gataaagttt gaagaaaagg
301tagaggaagg cggcgaacgc tggagcaagc cccacgtgtc cacactatcc ctgcacagcc
361tcttcgagct ccgtacctgc ctgcagacgg ggacggtgct gctggatttg gacagtggct
421ccttaccaca gatcatagat gatgtcattg agaagcagat tgaggatggt ctcctgcggc
481cagagctccg ggagagggtc agttacgtcc tcctgaggag gcaccgccac caaaccaaga
541agcccatcca ccgctcctta gctgacattg ggaagtcagt ctccaccaca aatcgcagtc
601ctgcccggag ccctggtgct ggcccgagtc tacaccactc cacggaagac ctgcggatgc
661ggcagagtgc aaattacgga cgtctgtgtc atgcccagag cagaagcatg aatgacattt
721ctctcacccc aaacacagac cagcggaaaa acaaattcat gaagaagatc cccaaggact
781cagaagcgtc caacgtgctc gtgggcgagg tggacttcct agaccagcca ttcatcgcgt
841tcgtgcgcct catccagtcg gccatgctgg gaggagtgac cgaggtgcct gtccccacca
901gatttctgtt tatactactg ggaccttctg ggagagcaaa atcctacaat gaaattggcc
961gtgccattgc aaccctcatg gtagatgatc tcttcagtga cgtggcctac aaagcccgca
1021atcgggaaga tctgatcgca ggaattgatg aatttctgga tgaggtcatc gtccttcctc
1081ctggagaatg ggacccaaat atccggattg agccccccaa gaaggtgccc tctgctgaca
1141agaggaaatc tgtgttctcc ctagcagagc tgggccagat gaatggctct gtgggaggag
1201gcggcggagc tcctggagga ggcaatggag gtggtggtgg tggtggcagt ggcggcgggg
1261ctggcagtgg cggggccggc ggaacaagca gcggggatga tggagagatg ccagccatgc
1321atgaaatcgg ggaggaactt atctggacag gaaggttctt cggtggcctg tgtctggata
1381tcaagaggaa gttgccctgg ttcccaagtg acttctatga tggcttccac attcagtcca
1441tctctgccat cctattcatc tacctcggct gtatcaccaa cgcgatcacc tttggtgggc
1501ttctggggga tgccaccgac aattatcagg gagtgatgga gagcttcctg ggcactgcca
1561tggctggctc cttgttctgc ctcttctcgg gacagcctct catcattctc agcagcacgg
1621ggcccatcct catctttgag aagctcctct tcgacttcag caaaggcaat ggcctggact
1681acatggagtt ccgcctctgg attggcctac actcagctgt ccagtgcctt atcctagtgg
1741ccacagatgc cagctttatc atcaaatata tcacccgctt caccgaggag ggcttctcca
1801cccttatcag cttcatcttc atctacgatg ccatcaagaa gatgatcggt gccttcaagt
1861actaccctat caatatggac ttcaagccaa acttcatcac tacctacaag tgcgagtgtg
1921tcgcccctga cacagtgaat acaaccgtgt tcaatgcttc agccccattg gcaccagaca
1981ccaacgcttc tctgtacaac ctccttaacc tcacagcgtt ggactggtcc ctgctgagca
2041agaaggagtg tctgagctac ggcgggcgcc tgcttgggaa ttcctgcaag tttatcccag
2101acctggcgct catgtccttc atccttttct ttgggacata ctccatgacc ctgaccctga
2161agaagttcaa attcagccgc tattttccta ccaagccaac gcggcctgac cgaggctggt
2221tcgtggcccc ctttgggaag aacccgtggt gggtataccc agcaagcatc ctgcccgccc
2281tgctggtgac catcctgatc ttcatggacc agcagatcac tgccgtcatt gtcaaccgga
2341aggagaacaa actgaagaag gctgccggct accatctgga cctgttctgg gtgggcatcc
2401tcatggcttt gtgctccttt atggggctcc cctggtacgt ggctgccacg gtcatctcca
2461tcgcccacat cgacagcctc aagatggaga cagagaccag tgcccctggg gagcagcccc
2521agtttctggg agtcagggaa cagagagtaa ccggcatcat cgtcttcatc ctgacgggaa
2581tctctgtctt cctggctccc atcctaaagt gtatccccct gccggtgctg tacggagtct
2641tcctctacat gggcgtggcc tccctgaatg gcatccagtt ctgggaacgc tgcaagctct
2701tcctgatgcc agccaagcac cagccggacc atgccttccT GCGGCACGTG CCGCTGCGCC
2761GGATCCACCT CTTCACCCTG GTGCAGATCC TCTGCCTGGC GGTGCTCTGG ATCCTCAAAT
2821CCACGGTGGC TGCCATCATC TTCCCGGTCA TGATCCTGGG CCTCATCATC GTTCGAAGGC
2881TTCTGGATTT CATCTTTTCC CAGCACGACC TGGCCTGGAT TGACAACATC CTCCCAGAGA
2941AGGAAAAAAA GGAGACAGAC AAGAAGAGGA AGAGAAAAAA AGGGGCCCAC GAGGACTGTG
3001ATGAGGAGCC CCAGTTCCCT CCTCCCTCGG TTATAAAGAT TCCCATGGAA AGTGTCCAAT
3061CAGATCCCCA AAACGGTATC CACTGCATTG CCAGAAAAAG ATCTTCCAGT TGGAGTTACT
3121CACTCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
3181TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
3241CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACCAAGCC CCCATACGAC CTATCACGCG
3301TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)

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