Transcript: Human NM_021196.3

Homo sapiens solute carrier family 4 member 5 (SLC4A5), transcript variant a, mRNA.

Source:
NCBI, updated 2019-06-27
Taxon:
Homo sapiens (human)
Gene:
SLC4A5 (57835)
Length:
3449
CDS:
3..3416

Additional Resources:

NCBI RefSeq record:
NM_021196.3
NBCI Gene record:
SLC4A5 (57835)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021196.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414761 ATCGCAGGAATTGATGAATTT pLKO_005 1161 CDS 100% 13.200 18.480 N SLC4A5 n/a
2 TRCN0000038176 CCCTATCAATATGGACTTCAA pLKO.1 1991 CDS 100% 4.950 6.930 N SLC4A5 n/a
3 TRCN0000038178 CCGGAAGGAGAACAAACTGAA pLKO.1 2576 CDS 100% 4.950 6.930 N SLC4A5 n/a
4 TRCN0000038177 CGACAATTATCAGGGAGTGAT pLKO.1 1643 CDS 100% 4.950 6.930 N SLC4A5 n/a
5 TRCN0000038174 GCCAGCTTTATCATCAAATAT pLKO.1 1875 CDS 100% 15.000 10.500 N SLC4A5 n/a
6 TRCN0000442365 ACCGCTCCTTAGCTGACATTG pLKO_005 676 CDS 100% 10.800 7.560 N SLC4A5 n/a
7 TRCN0000038175 CCCTCTTTACAGAGATGGATA pLKO.1 337 CDS 100% 4.950 3.465 N SLC4A5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021196.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12409 pDONR223 100% 89.6% 89.5% None 80_271del;2316_2429del;2917_2964del n/a
2 ccsbBroad304_12409 pLX_304 0% 89.6% 89.5% V5 80_271del;2316_2429del;2917_2964del n/a
3 TRCN0000467196 AACCAAGCCCCCATACGACCTATC pLX_317 12.8% 89.6% 89.5% V5 80_271del;2316_2429del;2917_2964del n/a
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