Construct: ORF TRCN0000467210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012225.1_s317c1
- Derived from:
- ccsbBroadEn_06241
- DNA Barcode:
- TATTTAGCACGCAAGAGCCGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRA5 (2558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | NM_000810.4 | 99.7% | 99.5% | 6C>G;606T>C;1381C>T |
| 2 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | NM_001165037.1 | 99.7% | 99.5% | 6C>G;606T>C;1381C>T |
| 3 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | XM_005268258.2 | 99.7% | 99.5% | 6C>G;606T>C;1381C>T |
| 4 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | XM_006720459.2 | 99.7% | 99.5% | 6C>G;606T>C;1381C>T |
| 5 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | XM_017022055.1 | 65.1% | 63.8% | (many diffs) |
| 6 | human | 2558 | GABRA5 | gamma-aminobutyric acid typ... | XM_017022056.1 | 65.1% | 63.8% | (many diffs) |
| 7 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | NM_176942.4 | 87.4% | 94.8% | (many diffs) |
| 8 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | XM_006540556.3 | 87.4% | 94.8% | (many diffs) |
| 9 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | XM_006540557.3 | 87.4% | 94.8% | (many diffs) |
| 10 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | XM_006540558.2 | 87.4% | 94.8% | (many diffs) |
| 11 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | XM_006540559.3 | 87.4% | 94.8% | (many diffs) |
| 12 | mouse | 110886 | Gabra5 | gamma-aminobutyric acid (GA... | XM_006540560.3 | 87.4% | 94.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1452
- ORF length:
- 1386
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaatggaatg ttctctggtt ttatcatgat caaaaacctc cttctctttt 121 gtatttccat gaacttatcc agtcactttg gcttttcaca gatgccaacc agttcagtga 181 aagatgagac caatgacaac atcacgatat ttaccaggat cttggatggg ctcttggatg 241 gctacgacaa cagacttcgg cccgggctgg gagagcgcat cactcaggtg aggaccgaca 301 tctacgtcac cagcttcggc ccggtgtccg acacggaaat ggagtacacc atagacgtgt 361 ttttccgaca aagctggaaa gatgaaaggc ttcggtttaa ggggcccatg cagcgcctcc 421 ctctcaacaa cctccttgcc agcaagatct ggaccccaga cacgttcttc cacaacggga 481 agaagtccat cgctcacaac atgaccacgc ccaacaagct gctgcggctg gaggacgacg 541 gcaccctgct ctacaccatg cgcttgacca tctctgcaga gtgccccatg cagcttgagg 601 acttcccgat ggatgcgcac gcttgccctc tgaaatttgg cagctatgcg taccctaatt 661 ctgaagtcgt ctacgtctgg accaacggct ccaccaagtc ggtggtggtg gcggaagatg 721 gctccagact gaaccagtac cacctgatgg ggcagacggt gggcactgag aacatcagca 781 ccagcacagg cgaatacaca atcatgacag ctcacttcca cctgaaaagg aagattggct 841 actttgtcat ccagacctac cttccctgca taatgaccgt gatcttatca caggtgtcct 901 tttggctgaa ccgggaatca gtcccagcca ggacagtttt tggggtcacc acggtgctga 961 ccatgacgac cctcagcatc agcgccagga actctctgcc caaagtggcc tacgccaccg 1021 ccatggactg gttcatagcc gtgtgctatg ccttcgtctt ctcggcgctg atagagtttg 1081 ccacggtcaa ttactttacc aagagaggct gggcctggga tggcaaaaaa gccttggaag 1141 cagccaagat caagaaaaag cgtgaagtca tactaaataa gtcaacaaac gcttttacaa 1201 ctgggaagat gtctcacccc ccaaacattc cgaaggaaca gaccccagca gggacgtcga 1261 atacaaccTC AGTCTCAGTA AAACCCTCTG AAGAGAAGAC TTCTGAAAGC AAAAAGACTT 1321 ACAACAGTAT CAGCAAAATT GACAAAATGT CCCGAATCGT ATTCCCAGTC TTGTTCGGCA 1381 CTTTCAACTT AGTTTACTGG GCAACGTATT TGAATAGGGA GCCGGTGATA AAAGGAGCCG 1441 CCTCTTCAAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1501 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1561 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAT TTAGCACGCA AGAGCCGGAA 1621 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t