Construct: ORF TRCN0000467223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009660.1_s317c1
- Derived from:
- ccsbBroadEn_15641
- DNA Barcode:
- TCCCTGGAGCACACAACACCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAMP4 (8674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_001185127.2 | 99.7% | 99.2% | 413A>C |
| 2 | human | 8674 | VAMP4 | vesicle associated membrane... | NM_003762.5 | 99% | 98.5% | 114_116delAAG;416A>C |
| 3 | human | 8674 | VAMP4 | vesicle associated membrane... | XM_017002713.2 | 70.2% | 52% | 114_116delAAG;217_218ins46;300_301ins77 |
| 4 | human | 8674 | VAMP4 | vesicle associated membrane... | NR_033704.2 | 15.6% | (many diffs) | |
| 5 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_001347125.1 | 93.3% | 96.4% | (many diffs) |
| 6 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496934.2 | 93.3% | 96.4% | (many diffs) |
| 7 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | NM_016796.3 | 89.3% | 92.1% | (many diffs) |
| 8 | mouse | 53330 | Vamp4 | vesicle-associated membrane... | XM_006496936.3 | 89.3% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tcccaagttt aagcgccacc tcaatgatga tgatgtcaca ggttctgtga 121 aaagtgaaag gagaaatctt tTGGAAGATG ATTCAGATGA AGAAGAGGAC TTTTTTCTGG 181 GACCATCTGG ACCAAGATTT GGACCTAGAA ATGATAAAAT TAAGCATGTT CAGAATCAAG 241 TGGATGAAGT TATTGATGTC ATGCAAGAAA ATATTACAAA GGTAATTGAG AGAGGGGAGA 301 GACTAGATGA ACTACAGGAC AAATCAGAAA GCTTATCGGA TAATGCAACA GCTTTTAGCA 361 ACAGATCCAA ACAACTTCGA AGGCAAATGT GGTGGCGTGG ATGCAAAATA AAAGCCATCA 421 TGGCTTTGGT TGCTGCTATC CTTTTGCTAG TGATTATCAT TCTTATAGTC ATGAAATCCC 481 GTACTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCCTGGAG CACACAACAC CTAACACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt