Construct: ORF TRCN0000467255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006574.1_s317c1
- Derived from:
- ccsbBroadEn_00479
- DNA Barcode:
- GGCCCACATTTACCTATTGACTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDN3 (1908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1908 | EDN3 | endothelin 3 | NM_207034.3 | 100% | 100% | |
| 2 | human | 1908 | EDN3 | endothelin 3 | XM_011528655.2 | 97.5% | 92.6% | 540_553del;601_604delAGAC |
| 3 | human | 1908 | EDN3 | endothelin 3 | XM_005260312.4 | 94.8% | 94.8% | 587_625del |
| 4 | human | 1908 | EDN3 | endothelin 3 | NM_207033.3 | 94.1% | 93.2% | 541_542insGTAATTCAAG;544_545ins32 |
| 5 | human | 1908 | EDN3 | endothelin 3 | XM_005260313.5 | 85.4% | 76.1% | 540_553del;636_637ins92 |
| 6 | human | 1908 | EDN3 | endothelin 3 | XM_006723734.3 | 83.9% | 82.1% | (many diffs) |
| 7 | human | 1908 | EDN3 | endothelin 3 | NM_207032.3 | 83% | 80.3% | 587_621del;657_658ins92 |
| 8 | human | 1908 | EDN3 | endothelin 3 | NM_001302455.2 | 82.1% | 82.3% | 587_590delAGAC;594_595ins124 |
| 9 | human | 1908 | EDN3 | endothelin 3 | XM_024451847.1 | 80.9% | 76.1% | 540_553del;603_604ins125 |
| 10 | human | 1908 | EDN3 | endothelin 3 | XM_024451848.1 | 80.9% | 76.1% | 540_553del;603_604ins125 |
| 11 | human | 1908 | EDN3 | endothelin 3 | NM_001302456.2 | 80.6% | 76.8% | 540_541ins46;576_577ins92 |
| 12 | human | 1908 | EDN3 | endothelin 3 | XR_002958461.1 | 56.7% | (many diffs) | |
| 13 | human | 1908 | EDN3 | endothelin 3 | XR_936513.2 | 56% | 1_397del;984_1076del;1165_1166ins39 | |
| 14 | human | 1908 | EDN3 | endothelin 3 | XR_002958462.1 | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccggggctg tggctccttt tcgggctcac agtgacctcc gccgcaggat 121 tcgtgccttg ctcccagtct ggggatgctg gcaggcgcgg cgtgtcccag gcccccactg 181 cagccagatc tgagggggac tgtgaagaga ctgtggctgg ccctggcgag gagactgtgg 241 ctggccctgg cgaggggact gtggccccga cagcactgca gggtccaagc ccTGGAAGCC 301 CTGGGCAGGA GCAGGCGGCC GAGGGGGCCC CTGAGCACCA CCGATCCAGG CGCTGCACGT 361 GCTTCACCTA CAAGGACAAG GAGTGTGTCT ACTATTGCCA CCTGGACATC ATTTGGATCA 421 ACACTCCCGA ACAGACGGTG CCCTATGGAC TGTCCAACTA CAGAGGAAGC TTCCGGGGCA 481 AGAGGTCTGC GGGGCCACTT CCAGGGAATC TGCAGCTCTC ACATCGGCCA CACTTGCGCT 541 GCGCTTGTGT GGGGAGATAT GACAAGGCCT GCCTGCACTT TTGCACCCAA ACTCTGGACG 601 TCAGCAGTAA TTCAAGGACG GCAGAAAAAA CAGACAAAGA AGAGGAAGGG AAGGTTGAAG 661 TCAAGGACCA ACAAAGCAAG CAGGCTTTAG ACCTCCACCA TCCAAAGCTC ATGCCCGGCA 721 GTGGACTCGC CCTCGCTCCA TCTACCTGCC CCCGCTGCCT CTTTCAGGAA GGAGCCCCTT 781 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGAGGCCC ACATTTACCT ATTGACTTGA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt