Construct: ORF TRCN0000467261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002621.1_s317c1
- Derived from:
- ccsbBroadEn_09361
- DNA Barcode:
- GATAACGAAGTGCTTTGCGACCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGB5 (93659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93659 | CGB5 | chorionic gonadotropin subu... | NM_033043.2 | 99.7% | 99.3% | 10T>C |
| 2 | human | 94115 | CGB8 | chorionic gonadotropin subu... | NM_033183.2 | 99.7% | 99.3% | 10T>C |
| 3 | human | 1082 | CGB3 | chorionic gonadotropin subu... | NM_000737.3 | 99.5% | 99.3% | 10T>C;450C>T |
| 4 | human | 94027 | CGB7 | chorionic gonadotropin subu... | NM_033142.1 | 98.7% | 97.5% | (many diffs) |
| 5 | human | 94027 | CGB7 | chorionic gonadotropin subu... | XM_024451784.1 | 98.7% | 97.5% | (many diffs) |
| 6 | human | 114336 | CGB2 | chorionic gonadotropin subu... | NM_033378.1 | 97.5% | 96.9% | (many diffs) |
| 7 | human | 114335 | CGB1 | chorionic gonadotropin subu... | NM_033377.1 | 92.9% | 92.1% | (many diffs) |
| 8 | human | 114336 | CGB2 | chorionic gonadotropin subu... | NM_001319065.1 | 90.9% | 90.9% | (many diffs) |
| 9 | human | 114336 | CGB2 | chorionic gonadotropin subu... | XM_005258479.3 | 89.8% | 88.3% | (many diffs) |
| 10 | human | 3972 | LHB | luteinizing hormone subunit... | NM_000894.2 | 78.6% | 68.8% | (many diffs) |
| 11 | human | 3972 | LHB | luteinizing hormone subunit... | XM_011526975.1 | 71% | 60.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 564
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatgctc caggggctgc tgctgttgct gctgctgagc atgggcggga 121 catgggcatc caaggagccg ctTCGGCCAC GGTGCCGCCC CATCAATGCC ACCCTGGCTG 181 TGGAGAAGGA GGGCTGCCCC GTGTGCATCA CCGTCAACAC CACCATCTGT GCCGGCTACT 241 GCCCCACCAT GACCCGCGTG CTGCAGGGGG TCCTGCCGGC CCTGCCTCAG GTGGTGTGCA 301 ACTACCGCGA TGTGCGCTTC GAGTCCATCC GGCTCCCTGG CTGCCCGCGC GGCGTGAACC 361 CCGTGGTCTC CTACGCCGTG GCTCTCAGCT GTCAATGTGC ACTCTGCCGC CGCAGCACCA 421 CTGACTGCGG GGGTCCCAAG GACCACCCCT TGACCTGTGA TGACCCCCGC TTCCAGGACT 481 CCTCTTCCTC AAAGGCCCCT CCCCCCAGCC TTCCAAGTCC ATCCCGACTC CCGGGGCCCT 541 CGGACACCCC GATCCTCCCA CAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ATAACGAAGT 721 GCTTTGCGAC CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att