Construct: ORF TRCN0000467276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005398.1_s317c1
- Derived from:
- ccsbBroadEn_10705
- DNA Barcode:
- ACCTTCAATTATTCTCGGTAAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA8 (767)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 767 | CA8 | carbonic anhydrase 8 | XM_011517588.3 | 90.5% | 88.1% | (many diffs) |
| 2 | human | 767 | CA8 | carbonic anhydrase 8 | XM_011517587.2 | 88.7% | 87.7% | (many diffs) |
| 3 | human | 767 | CA8 | carbonic anhydrase 8 | NM_001321839.2 | 57.6% | 55% | (many diffs) |
| 4 | human | 767 | CA8 | carbonic anhydrase 8 | NM_001321838.2 | 57.3% | 53.7% | (many diffs) |
| 5 | human | 767 | CA8 | carbonic anhydrase 8 | NM_001321837.2 | 52% | 48.7% | (many diffs) |
| 6 | human | 767 | CA8 | carbonic anhydrase 8 | NM_004056.6 | 52% | 48.7% | (many diffs) |
| 7 | human | 767 | CA8 | carbonic anhydrase 8 | XM_017013818.1 | 21.6% | 16% | (many diffs) |
| 8 | human | 767 | CA8 | carbonic anhydrase 8 | NR_135821.2 | 7.8% | (many diffs) | |
| 9 | mouse | 12319 | Car8 | carbonic anhydrase 8 | NM_007592.3 | 45% | 47.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaccTGAGC TTCATCGAAG ATACCGTCGC CTTCCCCGAG AAGGAAGAGG 121 ATGAGGAGGA AGAAGAGGAG GGTGTGGAGT GGGGCTACGA GGAAGGTGTT GAGTGGGGTC 181 TGGTGTTTCC TGATGCTAAT GGGGAATACC AGTCTCCTAT TAACCTAAAC TCAAGAGAGG 241 CTAGGTATGA CCCCTCGCTG TTGGATGTCC GCCTCTCCCC AAATTATGTG GTGTGCCGAG 301 ACTGTGAAGT CACCAATGAT GGACATACCA TTCAGGTTAT CCTGAAGTCA AAATCAGTTC 361 TTTCGGGAGG ACCATTGCCT CAAGGGCATG AGTTTGAACT GTACGAAGTG AGATTTCACT 421 GGGGAAGAGA AAACCAGCGT GGTTCTGAGC ACACGGTTAA TTTCAAAGCT TTTCCCATGG 481 AGGTAAGAAT AACAAATCAT CTTGTAAAAA TCTTGTTTTC TGAATACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA ACCTTCAATT ATTCTCGGTA AAATACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt