Construct: ORF TRCN0000467297
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016737.1_s317c1
- Derived from:
- ccsbBroadEn_08907
- DNA Barcode:
- TGTATCTAGCGAGGTCCATGAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHNAK (79026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467297
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_024060.4 | 99.7% | 99.3% | 337G>T |
| 2 | human | 79026 | AHNAK | AHNAK nucleoprotein | XM_017018270.1 | 2.4% | 2% | (many diffs) |
| 3 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001346445.2 | 2.3% | 2% | (many diffs) |
| 4 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001346446.2 | 2.3% | 2% | (many diffs) |
| 5 | human | 79026 | AHNAK | AHNAK nucleoprotein | NM_001620.3 | 2.3% | 2% | (many diffs) |
| 6 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_001039959.2 | 88.4% | 89.3% | (many diffs) |
| 7 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_001286518.1 | 76.6% | 76.8% | (many diffs) |
| 8 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NR_104460.1 | 33.1% | (many diffs) | |
| 9 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877684.3 | 30% | (many diffs) | |
| 10 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877685.3 | 26.9% | (many diffs) | |
| 11 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XR_877686.3 | 26.3% | (many diffs) | |
| 12 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_006527256.4 | 4% | 3.5% | (many diffs) |
| 13 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_017318272.2 | 2.8% | 2.5% | (many diffs) |
| 14 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | NM_009643.2 | 2.3% | 2% | (many diffs) |
| 15 | mouse | 66395 | Ahnak | AHNAK nucleoprotein (desmoy... | XM_006527253.4 | 2.3% | 2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaggaggag acaacccggg agctgctgct gcccaactgg cagggtagtg 121 gctcccacgg gctgaccatc gcccagaggg acgacggcgt ctttgtgcag gaggtgacgc 181 agaactcccc TGCGGCCCGC ACTGGGGTGG TCAAGGAGGG GGACCAGATT GTGGGTGCCA 241 CCATCTACTT TGACAACCTG CAGTCGGGTG AGGTGACCCA GCTGCTGAAC ACCATGGGGC 301 ACCACACGGT GGGCCTGAAG CTGCACCGCA AGGGGGACCG CTCTCCCGAG CCTGGCCAGA 361 CCTGGACCCG TGAAGTCTTC AGCTCCTGCA GCTCTGAAGT GTTTCTGAAC ACACCACAGC 421 CATCAGCACT GGAATGCAAA GACCAGAACA AACAGAAGGA AGCCAGCAGC CAAGCCGGGG 481 CAGTTTCAGT CTCCACCCCA AATGCAGGAC TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 661 TATCTAGCGA GGTCCATGAA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt