Transcript: Mouse XM_006527256.4

PREDICTED: Mus musculus AHNAK nucleoprotein (desmoyokin) (Ahnak), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Ahnak (66395)
Length:
11101
CDS:
258..10229

Additional Resources:

NCBI RefSeq record:
XM_006527256.4
NBCI Gene record:
Ahnak (66395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006527256.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000104947 TGGCTTGAAGTTGCACCGTAA pLKO.1 506 CDS 100% 4.050 5.670 N Ahnak n/a
2 TRCN0000440985 ACGCTGAGAGCTCGTGTTTAC pLKO_005 10545 3UTR 100% 10.800 7.560 N Ahnak n/a
3 TRCN0000449236 GGGACTCTGCAGCTAGGTAAA pLKO_005 10392 3UTR 100% 10.800 7.560 N Ahnak n/a
4 TRCN0000296590 TGGGTGCCACCATCTACTTTG pLKO_005 427 CDS 100% 10.800 7.560 N AHNAK n/a
5 TRCN0000104948 TGCCACCATCTACTTTGACAA pLKO.1 431 CDS 100% 4.950 3.465 N Ahnak n/a
6 TRCN0000439522 TGGATATCAAAGGCCCTAAAC pLKO_005 6250 CDS 100% 10.800 6.480 N Ahnak n/a
7 TRCN0000443627 TGGATATCAAAGGCCCTAAAG pLKO_005 3940 CDS 100% 10.800 6.480 N Ahnak n/a
8 TRCN0000127734 GCACTTGAAGATGCCCAAGAT pLKO.1 4766 CDS 100% 4.950 6.435 N AHNAK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006527256.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12531 pDONR223 100% 4% 3.5% None (many diffs) n/a
2 ccsbBroad304_12531 pLX_304 0% 4% 3.5% V5 (many diffs) n/a
3 TRCN0000471494 GCATGTACCGCGGGTTGGTACAGG pLX_317 67.8% 4% 3.5% V5 (many diffs) n/a
4 ccsbBroadEn_08907 pDONR223 100% 4% 3.5% None (many diffs) n/a
5 ccsbBroad304_08907 pLX_304 0% 4% 3.5% V5 (many diffs) n/a
6 TRCN0000467297 TGTATCTAGCGAGGTCCATGAACG pLX_317 71.3% 4% 3.5% V5 (many diffs) n/a
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