Construct: ORF TRCN0000467314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003246.1_s317c1
- Derived from:
- ccsbBroadEn_00135
- DNA Barcode:
- AGTCCTCGTGCTGAATCCCCGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0C (527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 527 | ATP6V0C | ATPase H+ transporting V0 s... | NM_001198569.1 | 100% | 100% | |
| 2 | human | 527 | ATP6V0C | ATPase H+ transporting V0 s... | NM_001694.4 | 100% | 100% | |
| 3 | mouse | 11984 | Atp6v0c | ATPase, H+ transporting, ly... | NM_009729.3 | 86.8% | 90.9% | (many diffs) |
| 4 | mouse | 100039636 | Atp6v0c-ps2 | ATPase, H+ transporting, ly... | NR_037854.1 | 35.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgagtccaag agcggccccg agtatgcttc gtttttcgcc gtcatgggcg 121 cctcggccgc catggtcttc agcgccctgg gcgctgccta tggcacagcc aagagcggta 181 ccggcattgc ggccatgtct gtcatgcggc cggagcagat catgaagtcc atcatcccag 241 tggtcatggc tggcatcatc gccatcTACG GCCTGGTGGT GGCAGTCCTC ATCGCCAACT 301 CCCTGAATGA CGACATCAGC CTCTACAAGA GCTTCCTCCA GCTGGGCGCC GGCCTGAGCG 361 TGGGCCTGAG CGGCCTGGCA GCCGGCTTTG CCATCGGCAT CGTGGGGGAC GCTGGCGTGC 421 GGGGCACCGC CCAGCAGCCC CGACTATTCG TGGGCATGAT CCTGATTCTC ATCTTCGCCG 481 AGGTGCTCGG CCTCTACGGT CTCATCGTCG CCCTCATCCT CTCCACAAAG TACCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGAAGTC CTCGTGCTGA ATCCCCGTCT ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt