Construct: ORF TRCN0000467320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017461.1_s317c1
- Derived from:
- ccsbBroadEn_02270
- DNA Barcode:
- TAACGCTCGCTATCCTTGATATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM20B (9917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | NM_001324310.1 | 98.7% | 98.7% | 1213_1227del |
| 2 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | NM_001324311.1 | 98.7% | 98.7% | 1213_1227del |
| 3 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | NM_014864.4 | 98.7% | 98.7% | 1213_1227del |
| 4 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | XM_017003001.2 | 93.5% | 93.5% | 377_445del;1282_1296del |
| 5 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | XM_017003002.1 | 93.5% | 93.5% | 377_445del;1282_1296del |
| 6 | human | 9917 | FAM20B | FAM20B glycosaminoglycan xy... | XR_002958229.1 | 70.8% | (many diffs) | |
| 7 | mouse | 215015 | Fam20b | family with sequence simila... | NM_145413.4 | 89.7% | 96% | (many diffs) |
| 8 | mouse | 215015 | Fam20b | family with sequence simila... | XM_006496736.2 | 89.7% | 96% | (many diffs) |
| 9 | mouse | 215015 | Fam20b | family with sequence simila... | XM_006496737.2 | 89.7% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctaaagcag cgagtcgtgc tgttagcaat tctccttgtc atttttatct 121 tcaccaaagt tttcctgatt gacaacttag atacatcagc tgccaaccgg gaggaccaga 181 gggcctttca ccgaatgatg actggcttgc gggtggagct ggcacccaag ctggaccata 241 ccttgcagtc tccctgggag attgcagccc agtgggtggt tccccgggaa gtgtaccctg 301 aagagacacc agagctgggg gcagtcatgc atgccatggc caccaagaaa atcattaaag 361 ctgatgtggg ttataaaggg acacagctga aagccttact gatacttgaa ggaggccaga 421 aagttgtttt caaacctaag cggtatagcc gagaccatgt ggtggaaggg gaaccgtatg 481 ctggttatga tagacacaat gcagaggtag cagcctttca cttggacagg attctgggtt 541 tccaccgagc ccccttggta gttggcagat ttgttaatct tcggacagag atcaaacctg 601 tcgccacaga gcagctgttg agcaccttcc taactgtagg aaacaatact tgtttttatg 661 ggaagtgcta ttactgccga gaaacagaac cagcttgtgc tgatggagac ataatggagg 721 gatctgtcac actttggctt ccagatgtgt ggcctctgca gaagcaccgt cacccatggg 781 gcaggactta ccgagaaggc aaattggcca ggtgggagta tgatgagagc tactgtgatg 841 ctgtgaagaa aacgtcccct tatgactctg gcccgcgcct cttggacatc attgacacag 901 ctgtctttga ttaccTGATT GGCAATGCTG ACCGCCATCA CTATGAGAGC TTTCAAGATG 961 ATGAAGGCGC TAGTATGCTC ATCCTTCTTG ATAATGCCAA AAGCTTTGGG AACCCCTCGC 1021 TGGATGAAAG AAGCATTCTT GCCCCTCTCT ATCAGTGTTG CATCATTCGG GTGTCCACCT 1081 GGAACAGACT GAACTACCTA AAGAATGGTG TGCTAAAGTC TGCCTTAAAA TCTGCCATGG 1141 CCCATGACCC CATCTCCCCA GTGCTCTCTG ATCCTCATCT GGACGCCGTG GACCAGCGGC 1201 TCCTGAGTGT CCTGGCCACC GTGAAGCAGT GCACCGACCA GTTTGGGATG GACACAGTAC 1261 TGGTGGAAGA CAGGATGCCT CTCTCACACT TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1441 ACGCTCGCTA TCCTTGATAT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt