Construct: ORF TRCN0000467321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008759.1_s317c1
- Derived from:
- ccsbBroadEn_11221
- DNA Barcode:
- TTGGCTGTAACTCGGGGTGTTTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT9B (7484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7484 | WNT9B | Wnt family member 9B | NM_001320458.2 | 99.8% | 99.6% | 317T>C |
| 2 | human | 7484 | WNT9B | Wnt family member 9B | NM_003396.3 | 87.4% | 85.1% | (many diffs) |
| 3 | human | 7484 | WNT9B | Wnt family member 9B | XM_011525178.2 | 80.7% | 77.7% | (many diffs) |
| 4 | mouse | 22412 | Wnt9b | wingless-type MMTV integrat... | NM_011719.4 | 76.2% | 77.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ccccccgccc gcgctggccc tggccgggct ctgcctgctg gcgctgcccg 121 ccgccgccgc ctcctacttc ggcctgaccg ggcgggaagt cctgacgccc ttcccaggat 181 tgggcactgc ggcagccccg gcacagggcg gggcccacct gaagcagtgt gacctgctga 241 agctgtcccg gcggcagaag cagctctgcc ggagggagcc cggcctggct gagaccctga 301 gggatgctgc gcacctcggc ctgcttgagt gccagtttca gttccggcat gagcgctgga 361 actgtagcct ggagggcagg acgggcctgc tcaagagagg cttcaaagag acagctttcc 421 tgtacgcggt gtcctctgcc gccctcaccc acaccctggc ccgggcctgc agcgctgggc 481 gcatggagcg ctgcacctgt gatgactctc cggggctgga gagccggcag gcctggcagt 541 ggggcgtgtg cggtgacaac ctcaagtaca gcaccaagtt tctgagcaac ttcctggggt 601 ccaagagagg aaacaaggac ctgcgggcac gggcagacgc ccacaatacc cacgtgggca 661 tcaaggctgt gaagagtggc cTCAGGACCA CGTGTAAGTG CCATGGCGTA TCAGGCTCCT 721 GTGCCGTGCG CACCTGCTGG AAGCAGCTCT CCCCGTTCCG TGAGACGGGC CAGGTGCTGA 781 AACTGCGCTA TGACTCGGCT GTCAAGGTGT CCAGTGCCAC CAATGAGGCC TTGGGCCGCC 841 TAGAGCTGTG GGCCCCTGCC AGGCAGGGCA GCCTCACCAA AGGCCTGGCC CCAAGGTCTG 901 GGGACCTGGT GTACATGGAG GACTCACCCA GCTTCTGCCG GCCCAGCAAG TACTCACCTG 961 GCACAGCAGG TTGGAGTGCA GTGGCAAGAT CTCAGCTCAT CACAACCTCC ACCTCCCGGA 1021 TTCAAGCGAT TCTCCCGTCT CAGCTGCCTG AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1201 GGCTGTAACT CGGGGTGTTT GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt