Construct: ORF TRCN0000467323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005795.1_s317c1
- Derived from:
- ccsbBroadEn_08339
- DNA Barcode:
- ATTATTATTCCCTTCCAGCCTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMPK1 (51727)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51727 | CMPK1 | cytidine/uridine monophosph... | NM_016308.3 | 99.8% | 99.5% | 22G>C |
| 2 | human | 51727 | CMPK1 | cytidine/uridine monophosph... | NM_001366135.1 | 85.9% | 85.9% | 0_1ins96 |
| 3 | human | 51727 | CMPK1 | cytidine/uridine monophosph... | NM_001136140.2 | 78.3% | 78% | 22G>C;170_171ins147 |
| 4 | human | 51727 | CMPK1 | cytidine/uridine monophosph... | NR_046394.2 | 20.6% | (many diffs) | |
| 5 | mouse | 66588 | Cmpk1 | cytidine monophosphate (UMP... | NM_025647.3 | 90% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gagccgctgc cgcagccggc tgctccacgt cctgggcctt agcttcctgc 121 tgcagacccg ccggccgatt ctcctctgct ctccacgtct catgaagccg ctggtcgtgt 181 tcgtcctcgg cggccccggc gccggcaagg ggacccagtg cgcccgcatc gtcgagaaat 241 atggctacac acacctttct gcaggagagc tgcttcgtga tgaaaggaag aacccagatt 301 cacagtatgg tgaacttatt GAAAAGTACA TTAAAGAAGG AAAGATTGTA CCAGTTGAGA 361 TAACCATCAG TTTATTAAAG AGGGAAATGG ATCAGACAAT GGCTGCCAAT GCTCAGAAGA 421 ATAAATTCTT GATTGATGGG TTTCCAAGAA ATCAAGACAA CCTTCAAGGA TGGAACAAGA 481 CCATGGATGG GAAGGCAGAT GTATCTTTCG TTCTCTTTTT TGACTGTAAT AATGAGATTT 541 GTATTGAACG ATGTCTTGAG AGGGGAAAGA GTAGTGGTAG GAGTGATGAC AACAGAGAGA 601 GCTTGGAAAA GAGAATTCAG ACCTACCTTC AGTCAACAAA GCCAATTATT GACTTATATG 661 AAGAAATGGG GAAAGTCAAG AAAATAGATG CTTCTAAATC TGTTGATGAA GTTTTTGATG 721 AAGTTGTGCA GATTTTTGAC AAGGAAGGCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATTAT 901 TATTCCCTTC CAGCCTTAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt