Construct: ORF TRCN0000467355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010394.1_s317c1
- Derived from:
- ccsbBroadEn_08620
- DNA Barcode:
- GCCCTATTTTCGAATCGCCCAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGB1 (56104)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56104 | PCDHGB1 | protocadherin gamma subfami... | NM_032095.1 | 99.9% | 100% | 1530G>T;1566C>T |
| 2 | human | 56104 | PCDHGB1 | protocadherin gamma subfami... | NM_018922.2 | 86.7% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2499
- ORF length:
- 2430
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagagagcc agagaagccg aaatgatgaa aagtcaggta ctgtttccct 121 tcctgctgtc tttgttctgc ggggccatct cccagcagat ccgatacacg attccagagg 181 agctagccaa cggctcacgg gtggggaaac ttgccaagga tctggggctc agtgtccggg 241 agttgccaac tcgaaaactg cgggttagtg cagaggatta tttcaacgtt agtttggaga 301 gcggggattt gttagtgaac ggtaggatag atcgagagaa gatttgcgga aggaaacttg 361 agtgtgcact agaattcgaa acggtcgctg aaaacccaat gaatgttttc cacgtggttg 421 ttgtaatcca agatattaat gacaatgcac cacgtttcgt tgcaaaaggc attgacttag 481 aaatttgtga gtcagcctta cccggggtaa aattctctct ggattctgct caagatgcag 541 atgtggaagg caattcactg aagttataca ccatcaaccc caatcaatac ttctctctgt 601 caacgaagga aagtcctgat ggaagtaaat atccggtatt actgctggaa aaacctctag 661 acagggaaca tcagagctct catcgcttaa tcctgactgc catggatggc ggggacccgc 721 ctctaagcgg caccacccat atctggatcc gagttacgga tgccaatgat aatgctcccg 781 tgtttagcca ggaggtatac agggttagcc tccaagaaaa cgtaccgtgg ggaacctccg 841 tgctgcgggt gatggccaca gaccaggatg agggcattaa tgcagagatc acctatgcct 901 tcctcaattc cccaataagt accagcctct tcaatctcaa tccaaatact ggcgacatca 961 caaccaatgg cacattggat tttgaagaga caagtagata tgtgttgagt gtggaagcta 1021 aggatggagg agtacacaca gctcactgta atgttcaaat agaaattgtt gacgagaatg 1081 acaatgcccc agaggtgaca ttcatgtcct tctctaacca gattccagag gattcagacc 1141 ttggaactgt aatagccctc ataaaagtgc gagacaagga ttctgggcaa aatggcatgg 1201 tgacatgcta tactcaggaa gaagttcctt tcaaattaga atccacctcg aagaattatt 1261 acaagctggt gattgctgga gccctaaacc gggagcagac agcagactac aacgtcacaa 1321 tcatagccac cgacaagggc aaaccagccc tttcctccag gacaagcatc accctgcaca 1381 tctccgacat caacgacaat gcacctgttt tccatcaggc ctcctatgtg gtccacgtgt 1441 ctgagaacaa cccacctggc gcctccattg cacaagtaag cgcctccgac ccggatttgg 1501 gacccaacgg cagagtctcc tactctattc tggccagtga cctggagccg cgggagctgt 1561 tgtcctacgt gtccgtgagc ccgcagagcg gggtggtttt cgcgcagcgc gccttcgacc 1621 acgagcagct gcgtgccttc gagctcacac tgcaggccag ggaccagggc tcccccgcgc 1681 tcagcgccaa cgtgagcctg cgcgtgttgg tgggcgacct caatgacaat gcgccacggg 1741 tgctgtaccc cgcgctgggg cctgatggct ccgccctctt cgatatggtg ccacgcgccg 1801 cagagcccgg ctacctggtg accaaggtgg tggcggtgga cgcagactca ggacacaacg 1861 cttggctgtc ctaccacgtg ctgcaggcca gcgagcccgg gctcttcagc ctggggttgc 1921 gcacgggtga ggtgcgcaca gcgcgtgcct tgggcgacag ggacgcggcc cgccagcgcc 1981 tgctggtcgc tgtgcgtgat ggaggacagc cgccactctc cgccaccgcc acgctgcacc 2041 taatcttcgc ggatagcctg caagaggtat tgccagacct cagcgaccgc cctgagccct 2101 ctgaccccca gacggaactg cagttttacc tggttgtggc cttggccttg atctcagtgc 2161 tctttctcct cgcggtgatt ctagcgatcg ccctgcgccT GCGACGTTCC TCCAGCCTCG 2221 ACACTGAGGG CTGCTTTCAA ACCGGTCTCT GCTCCAAGTC TGGGCCCGGG GTTCCTCCCA 2281 ACCACAGCGA GGGGACTTTG CCCTATTCCT ACAATCTATG TATTGCCTCT CATTCTGCAA 2341 AGACAGAGTT TAATTCTCTC AACCTGACAC CGGAAATGGC TCCCCCTCAG GATCTGCTGT 2401 GTGATGATCC TTCTATGGTT GTATGTGCCA GTAATGAAGA TCACAAAATC GCTTATGACC 2461 CTTCTTTGTC TTCGCACGTG AGTTTCTGCA AATCTAGTTT GCCAACTTTC TTGTACAAAG 2521 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2581 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2641 ACGAGCCCTA TTTTCGAATC GCCCAAATAC GCGTTAAGTC gacaatcaac ctctggatta 2701 caaaatttgt gaaagatt