Construct: ORF TRCN0000467381
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001797.1_s317c1
- Derived from:
- ccsbBroadEn_00158
- DNA Barcode:
- CTTTACTACACTGCTCCGGAAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCS1L (617)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467381
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001079866.2 | 100% | 100% | |
| 2 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001257342.2 | 100% | 100% | |
| 3 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001257343.2 | 100% | 100% | |
| 4 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001257344.2 | 100% | 100% | |
| 5 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001320717.2 | 100% | 100% | |
| 6 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371443.1 | 100% | 100% | |
| 7 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371444.1 | 100% | 100% | |
| 8 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371446.1 | 100% | 100% | |
| 9 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371447.1 | 100% | 100% | |
| 10 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371448.1 | 100% | 100% | |
| 11 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371449.1 | 100% | 100% | |
| 12 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371450.1 | 100% | 100% | |
| 13 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001374085.1 | 100% | 100% | |
| 14 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_004328.5 | 100% | 100% | |
| 15 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001318836.2 | 71.3% | 71.3% | 0_1ins360 |
| 16 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371451.1 | 71.3% | 71.3% | 0_1ins360 |
| 17 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371452.1 | 60.1% | 60.1% | 0_1ins501 |
| 18 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371453.1 | 60.1% | 60.1% | 0_1ins501 |
| 19 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371454.1 | 60.1% | 60.1% | 0_1ins501 |
| 20 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371455.1 | 60.1% | 60.1% | 0_1ins501 |
| 21 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001371456.1 | 60.1% | 60.1% | 0_1ins501 |
| 22 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NM_001374086.1 | 60.1% | 60.1% | 0_1ins501 |
| 23 | human | 617 | BCS1L | BCS1 homolog, ubiquinol-cyt... | NR_163955.1 | 53.7% | 1_1012del;1731_1732insCACAG;2265_2324del | |
| 24 | mouse | 66821 | Bcs1l | BCS1-like (yeast) | NM_001305652.1 | 89.1% | 93.7% | (many diffs) |
| 25 | mouse | 66821 | Bcs1l | BCS1-like (yeast) | NM_025784.5 | 89.1% | 93.7% | (many diffs) |
| 26 | mouse | 66821 | Bcs1l | BCS1-like (yeast) | XR_001785495.1 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc actttcagac tttattctgg ctctgaagga caatccctac tttggggctg 121 gatttgggct ggtgggtgtg ggcacagccc tggccctggc ccggaagggt gtccaactgg 181 gcctggtggc attccggcgc cattacatga tcacactgga agtccctgct cgagacagga 241 gctatgcctg gttgcttagc tggctcaccc gccacagtac ccgtactcag cacctcagtg 301 tcgagacttc gtaccttcag catgagagtg gccgcatttc cactaagttt gaatttgtcc 361 ccagccctgg aaaccatttt atctggtatc gggggaaatg gattcgggta gaacgaagtc 421 gagagatgca gatgatagac ttgcagacgg ggactccttg ggaatctgtc accttcacgg 481 ccctgggcac tgaccgaaag gttttcttca acatcctgga ggaagctcga gagctagcct 541 tgcagcagga ggaagggaag accgtgatgt acacagctgt gggctctgaa tggcgtccct 601 ttggctatcc acgccgccgg cgaccactga attctgtggt tctacaacag ggtctggctg 661 accgaattgt cagagacgtc caggaattca tcgataaccc caagtggtac actgacagag 721 gcattcctta cagacgtggc tacctgcttt atgggccccc tggttgcgga aagagcagtt 781 ttatcacagc cctggctggg gaactggagc acagcatctg cctgctgagc ctcacggact 841 ccagcctctc tgatgaccga ctcaaccacc tgctgagcgt ggccccgcag cagagcctgg 901 tactcctgga ggatgtggat gctgcttttc tcagtcgaga cttgGCTGTG GAGAACCCAG 961 TAAAGTACCA AGGCCTAGGT CGCCTCACCT TCAGTGGACT GCTCAATGCC TTGGATGGTG 1021 TGGCTTCCAC CGAGGCCCGC ATCGTGTTCA TGACCACCAA CCACGTTGAC AGGCTGGACC 1081 CTGCCCTGAT ACGCCCGGGG CGAGTGGACC TGAAGGAGTA CGTGGGCTAC TGCTCACACT 1141 GGCAGCTGAC CCAGATGTTC CAGAGGTTCT ATCCAGGGCA GGCACCTTCC TTAGCTGAGA 1201 ACTTTGCAGA ACATGTCCTT CGAGCTACAA ACCAGATCAG TCCTGCCCAG GTGCAGGGCT 1261 ACTTCATGCT GTATAAAAAT GACCCTGTAG GGGCAATTCA CAATGCTGAG TCTCTGAGGA 1321 GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACT TTACTACACT GCTCCGGAAG TGACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt