Construct: ORF TRCN0000467387
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015292.1_s317c1
- Derived from:
- ccsbBroadEn_14177
- DNA Barcode:
- TTTAAGAATTCTATTTCCAAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF125 (54941)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467387
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54941 | RNF125 | ring finger protein 125 | NM_017831.4 | 99.1% | 99.5% | 690_692delCAC;696_697insGCG |
| 2 | human | 54941 | RNF125 | ring finger protein 125 | XM_011526045.3 | 90.6% | 82.8% | (many diffs) |
| 3 | human | 54941 | RNF125 | ring finger protein 125 | XM_011526046.3 | 83.6% | 84% | 504_505ins108;582_584delCAC;588_589insGCG |
| 4 | human | 54941 | RNF125 | ring finger protein 125 | XM_011526047.3 | 75.9% | 71% | (many diffs) |
| 5 | mouse | 67664 | Rnf125 | ring finger protein 125 | XM_006526188.3 | 84% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 762
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctccgtgctg agcaccgaca gcggcaaatc ggcgcccgcc tctgccaccg 121 cgcgggccct ggagcgcagg agggacccgg agttgcccgt cacgtccttc gactgcgccg 181 tgtgccttga ggtgttacac cagcctgtcc ggacccgctg tggccacgta ttctgccgtt 241 cctgtattgc taccagtcta aagaacaaca agtggacctg tccttattgc cgggcataTC 301 TTCCTTCAGA AGGAGTTCCA GCAACTGATG TAGCCAAAAG AATGAAATCA GAGTATAAGA 361 ACTGCGCTGA GTGTGACACC CTGGTTTGCC TCAGTGAAAT GAGGGCACAT ATTCGGACTT 421 GTCAGAAGTA CATAGATAAG TATGGACCAC TACAAGAACT TGAGGAGACA GCAGCAAGGT 481 GTGTATGTCC CTTTTGTCAG AGGGAACTGT ATGAAGACAG CTTGCTGGAT CATTGTATTA 541 CTCATCACAG ATCGGAACGG AGGCCTGTGT TCTGTCCACT TTGCCGTTTA ATACCCGATG 601 AGAATCCAAG CAGCTTCAGT GGCAGTTTAA TAAGACATCT GCAAGTTAGT CACACTTTGT 661 TTTATGATGA TTTCATAGAT TTTAATATAA TTGAGGAAGC TCTTATCCGA AGAGTCTTAG 721 ACCGGTCACT TCTTGAATAT GTGAATCACT CGAACACAGC GTACCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGATTT AAGAATTCTA TTTCCAAAAT GACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t