Construct: ORF TRCN0000467418
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012533.1_s317c1
- Derived from:
- ccsbBroadEn_08444
- DNA Barcode:
- AATCTCTAACTGCCTTATGGTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZSCAN2 (54993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467418
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | NM_017894.6 | 99.7% | 99.3% | 418G>T |
| 2 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | NM_001007072.2 | 92.5% | 91.3% | (many diffs) |
| 3 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | XM_017022393.2 | 88% | 82% | (many diffs) |
| 4 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | XM_024449977.1 | 82.6% | 81.7% | 407_499del;511G>T |
| 5 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | NM_181877.4 | 23.8% | 22% | (many diffs) |
| 6 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | XM_024449975.1 | 23.8% | 22% | (many diffs) |
| 7 | human | 54993 | ZSCAN2 | zinc finger and SCAN domain... | XM_024449978.1 | 22.7% | 21.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 516
- ORF length:
- 450
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggctgcagac atcccgagag tgaccactcc gctgagctcc ttggtccagg 121 tgccTCAAGA GGAAGATAGA CAGGAGGAGG AGGTCACCAC CATGATCCTG GAGGATGACT 181 CCTGGGTGCA AGAAGCTGTG CTGCAGGAGG ATGGCCCTGA GTCTGAGCCC TTTCCCCAGA 241 GTGCTGGCAA GGGCGGCCCC CAGGAGGAGG TGACCAGGGG ACCACAGGGT GCACTCGGCC 301 GCCTCCGAGA GCTCTGCCGG CGCTGGCTGA GACCAGAGGT ACACACCAAG GAGCAGATGT 361 TAACCATGCT GCCAAAGGAA ATTCAGGCTT GGCTGCAAGA GCATCGGCCT GAAAGCAGTG 421 AGGAGGCAGC GGCCCTGGTG GAAGACTTGA CCCAGACCCT TCAGGACAGT GCTGTAGCTG 481 TCTTTGCTTC CCTTCCGGTG GAGGTGACCA GTTTGTACCC AACTTTCTTG TACAAAGTGG 541 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 601 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 661 AAATCTCTAA CTGCCTTATG GTTAGACGCG TTAAGTCgac aatcaacctc tggattacaa 721 aatttgtgaa agatt