Construct: ORF TRCN0000467439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012464.1_s317c1
- Derived from:
- ccsbBroadEn_07103
- DNA Barcode:
- CGCAATAACCGCTACCACTGAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSHB (7252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7252 | TSHB | thyroid stimulating hormone... | NM_000549.5 | 99.7% | 99.2% | 40A>G |
| 2 | human | 7252 | TSHB | thyroid stimulating hormone... | XM_011542065.2 | 99.7% | 99.2% | 40A>G |
| 3 | human | 7252 | TSHB | thyroid stimulating hormone... | NM_001277991.1 | 65% | 60.8% | (many diffs) |
| 4 | mouse | 22094 | Tshb | thyroid stimulating hormone... | NM_001165939.1 | 83.6% | 85.5% | (many diffs) |
| 5 | mouse | 22094 | Tshb | thyroid stimulating hormone... | NM_001165940.1 | 83.6% | 85.5% | (many diffs) |
| 6 | mouse | 22094 | Tshb | thyroid stimulating hormone... | NM_009432.2 | 83.6% | 85.5% | (many diffs) |
| 7 | mouse | 22094 | Tshb | thyroid stimulating hormone... | XM_006501301.3 | 83.6% | 85.5% | (many diffs) |
| 8 | mouse | 22094 | Tshb | thyroid stimulating hormone... | XM_006501304.3 | 83.6% | 85.5% | (many diffs) |
| 9 | mouse | 22094 | Tshb | thyroid stimulating hormone... | XM_006501305.3 | 83.6% | 85.5% | (many diffs) |
| 10 | mouse | 22094 | Tshb | thyroid stimulating hormone... | XM_017319535.1 | 83.6% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgctctcttt ctgatgtcca tgctttttGG CCTTGCATGT GGGCAAGCGA 121 TGTCTTTTTG TATTCCAACT GAGTATACAA TGCACATCGA AAGGAGAGAG TGTGCTTATT 181 GCCTAACCAT CAACACCACC ATCTGTGCTG GATATTGTAT GACACGGGAT ATCAATGGCA 241 AACTGTTTCT TCCCAAATAT GCTCTGTCCC AGGATGTTTG CACATATAGA GACTTCATCT 301 ACAGGACTGT AGAAATACCA GGATGCCCAC TCCATGTTGC TCCCTATTTT TCCTATCCTG 361 TTGCTTTAAG CTGTAAGTGT GGCAAGTGCA ATACTGACTA TAGTGACTGC ATACATGAAG 421 CCATCAAGAC AAACTACTGT ACCAAACCTC AGAAGTCTTA TCTGGTAGGA TTTTCTGTCT 481 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGACGCAA TAACCGCTAC CACTGAGTGA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt