Construct: ORF TRCN0000467451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002753.1_s317c1
- Derived from:
- ccsbBroadEn_15943
- DNA Barcode:
- TCGGCCCATTGCACAGCGAACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPAL3 (57185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322865.2 | 100% | 100% | |
| 2 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322868.1 | 62.4% | 59.5% | (many diffs) |
| 3 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001330409.1 | 60.2% | 57.4% | (many diffs) |
| 4 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | XM_017001869.1 | 60.2% | 57.4% | (many diffs) |
| 5 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322854.1 | 54.6% | 52.1% | (many diffs) |
| 6 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322855.1 | 54.6% | 52.1% | (many diffs) |
| 7 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322856.1 | 54.6% | 52.1% | (many diffs) |
| 8 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322857.1 | 54.6% | 52.1% | (many diffs) |
| 9 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_020448.5 | 54.6% | 52.1% | (many diffs) |
| 10 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | XM_017001868.1 | 53.4% | 50% | (many diffs) |
| 11 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322866.1 | 52.4% | 50% | (many diffs) |
| 12 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | XM_011541808.2 | 49.2% | 45.8% | (many diffs) |
| 13 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322864.1 | 48.6% | 45.4% | (many diffs) |
| 14 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322862.1 | 34.4% | 32.2% | (many diffs) |
| 15 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322863.1 | 34.4% | 32.2% | (many diffs) |
| 16 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322858.1 | 15% | 13.1% | (many diffs) |
| 17 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322859.1 | 15% | 13.1% | (many diffs) |
| 18 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322860.1 | 15% | 13.1% | (many diffs) |
| 19 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NM_001322861.1 | 15% | 13.1% | (many diffs) |
| 20 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NR_136508.1 | 12.7% | (many diffs) | |
| 21 | human | 57185 | NIPAL3 | NIPA like domain containing 3 | NR_136506.1 | 12% | (many diffs) | |
| 22 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | XM_006539232.1 | 56.3% | 55% | (many diffs) |
| 23 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | XM_006539231.1 | 56.1% | 54.9% | (many diffs) |
| 24 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | NM_028995.3 | 47.8% | 46.8% | (many diffs) |
| 25 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | XM_017320417.1 | 47.8% | 46.8% | (many diffs) |
| 26 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | NM_001347220.1 | 46.8% | 45.9% | (many diffs) |
| 27 | mouse | 74552 | Nipal3 | NIPA-like domain containing 3 | XM_006539230.3 | 46.8% | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cggatcccac agcgcagccc tgaagctgca gcagctgcct cccacaagta 121 gctccagcgc cgtaagcgag gcctccttct cctacaagga aaacctgatt ggcgccctct 181 tggcgatctt cgggcacctc gtggtcagca ttgcacttaa cctccagaag tactgccaca 241 tccgcctggc aggctccaag gatccccggg cctatttcaa gaccaagaca tggtggctgg 301 gcctgttcct gatgcttctg ggcgagctgg gtgtgttcgc cTCCTACGCC TTCGCGCCGC 361 TGTCACTCAT CGTGCCCCTC AGCGCAGTTT CTGTGATAGC TAGTGCCATC ATAGGAATCA 421 TATTCATCAA GGAAAAGTGG AAACCGAAAG ACTTTCTGAG GCGCTACGTC TTGTCCTTTG 481 TTGGCTGCGG TTTGGCTGTC GTGGGTACCT ACCTGCTGGT GACATTCGCA CCCAACAGTC 541 ACGAGAAGAT GACAGGCGAG AATGTCACCA GGCACCTCGT GAGCTGGCCT TTCCTTTTGT 601 ACATGCTGGT GGAGATCATT CTGTTCTGCT TGCTGCTCTA CTTCTACAAG GAGAAGAACG 661 CCAACAACAT TGTCGTGATT CTTCTCTTGG TGGCGTTACT TGTCTTGCTC TGTCACCCAG 721 GCTGGAGTGC AGTGGTACAA TCTTACCAAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CGGCCCATTG 901 CACAGCGAAC AACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att