Construct: ORF TRCN0000467502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011077.1_s317c1
- Derived from:
- ccsbBroadEn_11045
- DNA Barcode:
- AGCTTCCAACACCTCCGAGACAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PON2 (5445)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5445 | PON2 | paraoxonase 2 | NM_000305.3 | 61.7% | 60.4% | 443C>G;643_695del;711_1062del |
| 2 | human | 5445 | PON2 | paraoxonase 2 | NM_001018161.1 | 58.3% | 57% | (many diffs) |
| 3 | human | 5445 | PON2 | paraoxonase 2 | XM_005250453.1 | 42.5% | 39.2% | (many diffs) |
| 4 | human | 5445 | PON2 | paraoxonase 2 | XM_017012357.2 | 41.3% | 38.9% | (many diffs) |
| 5 | human | 5445 | PON2 | paraoxonase 2 | XM_017012358.2 | 37.4% | 36.1% | (many diffs) |
| 6 | mouse | 330260 | Pon2 | paraoxonase 2 | NM_183308.2 | 53.8% | 53.9% | (many diffs) |
| 7 | mouse | 330260 | Pon2 | paraoxonase 2 | XM_006505122.1 | 32.7% | 32.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 723
- ORF length:
- 657
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcggctggtg gctgtgggct tgctggggat cgcgctggcg ctcctgggcg 121 agaggcttct ggcactcaga aatcgactta aagcctccag agaagtagaa tctgtagacc 181 ttccacactg ccacctgatt aaaggaattg aagctggctc tgaagatatt gacatacttc 241 ccaatggtct GGCTTTTTTT AGTGTGGGTC TAAAATTCCC AGGACTCCAC AGCTTTGCAC 301 CAGATAAGCC TGGAGGAATA CTAATGATGG ATCTAAAAGA AGAAAAACCA AGGGCACGGG 361 AATTAAGAAT CAGTCGTGGG TTTGATTTGG CCTCATTCAA TCCACATGGC ATCAGCACTT 421 TCATAGACAA CGATGACACA GTTTATCTCT TTGTTGTAAA CCACCCAGAA TTCAAGAATA 481 CAGTGGAAAT TTTTAAATTT GAAGAAGGAG AAAATTCTCT GTTGCATCTG AAAACAGTCA 541 AACATGAGCT TCTTCCAAGT GTGAATGACA TCACAGCTGT TGGACCGGCA CATTTCTATG 601 CCACAAATGA CCACTACTTC TCTGATCCTT TCTTAAAGTA TTTAGAAACA TACTTGAACT 661 TACACTGGGC AAATGTTGTT TACTACAGTC CAAATGAAGT TAAAGTGGTA TATCTATGTT 721 GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 781 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 841 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CTTCCAACAC CTCCGAGACA GTACGCGTTA 901 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt