Construct: ORF TRCN0000467530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011329.1_s317c1
- Derived from:
- ccsbBroadEn_14265
- DNA Barcode:
- TAATTGTAGAAGAGTACATATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BEND5 (79656)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001302082.1 | 99.7% | 99.6% | 6C>A;226C>A |
| 2 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349793.1 | 99.7% | 99.6% | 6C>A;226C>A |
| 3 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349794.1 | 99.7% | 99.6% | 6C>A;226C>A |
| 4 | human | 79656 | BEND5 | BEN domain containing 5 | XM_011542142.2 | 99.7% | 99.6% | 6C>A;226C>A |
| 5 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349795.1 | 74.8% | 74.7% | 1_252del;258C>A;478C>A |
| 6 | human | 79656 | BEND5 | BEN domain containing 5 | XM_011542141.3 | 74.8% | 74.7% | 1_252del;258C>A;478C>A |
| 7 | human | 79656 | BEND5 | BEN domain containing 5 | XM_017002331.1 | 68.4% | 68.3% | 1_345del;351C>A;571C>A |
| 8 | human | 79656 | BEND5 | BEN domain containing 5 | NM_024603.4 | 59.6% | 59.6% | 1_507del;513C>A;733C>A |
| 9 | human | 79656 | BEND5 | BEN domain containing 5 | NR_146232.1 | 32.7% | (many diffs) | |
| 10 | human | 79656 | BEND5 | BEN domain containing 5 | XR_001737408.2 | 31.1% | (many diffs) | |
| 11 | human | 79656 | BEND5 | BEN domain containing 5 | XM_017002333.2 | 22.3% | 19.6% | (many diffs) |
| 12 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_011240594.2 | 94.7% | 99.2% | (many diffs) |
| 13 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_017320366.1 | 94.7% | 99.2% | (many diffs) |
| 14 | mouse | 67621 | Bend5 | BEN domain containing 5 | NM_026279.3 | 56.6% | 59.3% | (many diffs) |
| 15 | mouse | 67621 | Bend5 | BEN domain containing 5 | NR_033793.1 | 37.5% | (many diffs) | |
| 16 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_006503323.3 | 29.1% | 30.8% | (many diffs) |
| 17 | mouse | 67621 | Bend5 | BEN domain containing 5 | XR_376343.3 | 18.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aagtctagaa gatgctgtgg tgccccgggc tctgtatgag gagctgctgc 121 gcaactacca gcagcaacag gaagagatgc gccacctcca gcaggagctg gagcggactc 181 ggaggcagct ggtacaacag gccaagaagc tcaaggagta cggggcactt gtgtctgaaa 241 tgaaggagct ccgtgacctt aaccggaggc tccaggacgt gctgctcctg aggcttggca 301 gcggtcccgc cattgatctg gaaaaagtaa agtcagaatg tctcgagccc gagccggagt 361 tacggagcac tttcagtgag gaagcaaata cgtcgtccta ttaccccgct ccTGCGCCTG 421 TCATGGACAA GTATATCCTA GACAATGGCA AGGTCCATCT GGGAAGCGGG ATTTGGGTTG 481 ATGAGGAGAA ATGGCACCAG CTACAAGTAA CCCAAGGAGA TTCCAAGTAC ACGAAGAACT 541 TGGCAGTTAT GATTTGGGGA ACAGATGTTC TGAAAAACAG AAGCGTCACA GGCGTCGCCA 601 CAAAAAAAAA GAAAGATGCA GTCCCTAAAC CACCCCTCTC GCCTCACAAA CTAAGCATCG 661 TCAGAGAGTG TTTGTATGAC AGAATAGCAC AAGAAACTGT GGATGAAACT GAAATTGCAC 721 AGAGACTCTC CAAAGTCAAC AAGTACATCT GTGAAAAAAT CATGGATATC AATAAATCCT 781 GTAAAAATGA AGAACGAAGG GAAGCAAAAT ACAATTTGCA ATACCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGATAA TTGTAGAAGA GTACATATTT AACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t