Transcript: Human NR_146232.1

Homo sapiens BEN domain containing 5 (BEND5), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-06-12
Taxon:
Homo sapiens (human)
Gene:
BEND5 (79656)
Length:
1700
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146232.1
NBCI Gene record:
BEND5 (79656)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146232.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415677 CACGAAGAACTTGGCAGTTAT pLKO_005 1043 3UTR 100% 13.200 9.240 N BEND5 n/a
2 TRCN0000421616 AGAAAGATGCAGTCCCTAAAC pLKO_005 1123 3UTR 100% 10.800 7.560 N BEND5 n/a
3 TRCN0000128364 GAGAGTGTTTGTATGACAGAA pLKO.1 1177 3UTR 100% 4.950 3.465 N BEND5 n/a
4 TRCN0000129441 GCATCGTCAGAGAGTGTTTGT pLKO.1 1168 3UTR 100% 4.950 3.465 N BEND5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146232.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08942 pDONR223 100% 60.1% None (many diffs) n/a
2 ccsbBroad304_08942 pLX_304 0% 60.1% V5 (many diffs) n/a
3 TRCN0000470324 CTAGTTCACTCCTCAGTACGAGAT pLX_317 28.7% 60.1% V5 (many diffs) n/a
4 ccsbBroadEn_14265 pDONR223 100% 32.7% None (many diffs) n/a
5 ccsbBroad304_14265 pLX_304 0% 32.7% V5 (many diffs) n/a
6 TRCN0000467530 TAATTGTAGAAGAGTACATATTTA pLX_317 54.1% 32.7% V5 (many diffs) n/a
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