Construct: ORF TRCN0000467550
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013320.1_s317c1
- Derived from:
- ccsbBroadEn_10698
- DNA Barcode:
- GTATCGAGCTCTGCGTGTCACTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DST (667)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467550
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 667 | DST | dystonin | NM_001723.6 | 42.7% | 41.9% | (many diffs) |
| 2 | human | 667 | DST | dystonin | XM_017011225.2 | 34.6% | 33.1% | (many diffs) |
| 3 | human | 667 | DST | dystonin | NM_015548.5 | 21.8% | 21.5% | (many diffs) |
| 4 | human | 667 | DST | dystonin | XM_017011224.2 | 20.5% | 19.8% | (many diffs) |
| 5 | human | 667 | DST | dystonin | NM_183380.3 | 20.1% | 19.3% | (many diffs) |
| 6 | human | 667 | DST | dystonin | NM_001144770.1 | 19.9% | 19.2% | (many diffs) |
| 7 | human | 667 | DST | dystonin | XM_017011223.1 | 19.6% | 18.9% | (many diffs) |
| 8 | human | 667 | DST | dystonin | XM_017011222.2 | 19.6% | 18.8% | (many diffs) |
| 9 | human | 667 | DST | dystonin | XM_017011221.1 | 19.5% | 18.7% | (many diffs) |
| 10 | human | 667 | DST | dystonin | NM_001144769.3 | 19.4% | 18.7% | (many diffs) |
| 11 | human | 667 | DST | dystonin | XM_011514825.3 | 19.4% | 18.7% | (many diffs) |
| 12 | human | 667 | DST | dystonin | XM_017011220.1 | 19.4% | 18.6% | (many diffs) |
| 13 | human | 667 | DST | dystonin | XM_017011219.1 | 19.2% | 18.5% | (many diffs) |
| 14 | human | 667 | DST | dystonin | XM_005249324.3 | 15.5% | 15.3% | (many diffs) |
| 15 | human | 667 | DST | dystonin | XM_011514826.3 | 14.8% | 14.2% | (many diffs) |
| 16 | human | 667 | DST | dystonin | XM_024446530.1 | 14.5% | 14% | (many diffs) |
| 17 | human | 667 | DST | dystonin | XM_005249323.4 | 14.5% | 14% | (many diffs) |
| 18 | human | 667 | DST | dystonin | XM_017011217.1 | 14.5% | 13.9% | (many diffs) |
| 19 | human | 667 | DST | dystonin | XM_005249322.5 | 14.4% | 13.9% | (many diffs) |
| 20 | human | 667 | DST | dystonin | XM_005249320.4 | 14.4% | 13.9% | (many diffs) |
| 21 | human | 667 | DST | dystonin | XM_005249319.2 | 14.4% | 13.9% | (many diffs) |
| 22 | human | 667 | DST | dystonin | XM_017011216.2 | 14.4% | 13.9% | (many diffs) |
| 23 | human | 667 | DST | dystonin | XM_005249318.2 | 14.4% | 13.8% | (many diffs) |
| 24 | human | 667 | DST | dystonin | XM_017011215.2 | 14.4% | 13.8% | (many diffs) |
| 25 | human | 667 | DST | dystonin | XM_017011214.2 | 14.3% | 13.8% | (many diffs) |
| 26 | human | 667 | DST | dystonin | XM_017011213.1 | 14.3% | 13.8% | (many diffs) |
| 27 | human | 667 | DST | dystonin | XM_005249316.3 | 14.3% | 13.7% | (many diffs) |
| 28 | human | 667 | DST | dystonin | XM_017011212.1 | 14.2% | 13.7% | (many diffs) |
| 29 | human | 667 | DST | dystonin | XM_017011211.2 | 14.2% | 13.7% | (many diffs) |
| 30 | human | 667 | DST | dystonin | XM_005249315.3 | 14.2% | 13.6% | (many diffs) |
| 31 | human | 667 | DST | dystonin | XM_017011210.1 | 14.2% | 13.6% | (many diffs) |
| 32 | human | 667 | DST | dystonin | XM_011514824.2 | 14.1% | 13.6% | (many diffs) |
| 33 | human | 667 | DST | dystonin | XM_017011209.1 | 14.1% | 13.6% | (many diffs) |
| 34 | human | 667 | DST | dystonin | XM_017011208.1 | 14.1% | 13.6% | (many diffs) |
| 35 | human | 667 | DST | dystonin | XM_017011207.1 | 14.1% | 13.6% | (many diffs) |
| 36 | human | 667 | DST | dystonin | XM_005249310.4 | 14.1% | 13.6% | (many diffs) |
| 37 | human | 667 | DST | dystonin | XM_017011206.1 | 14.1% | 13.6% | (many diffs) |
| 38 | human | 667 | DST | dystonin | XM_017011205.1 | 14.1% | 13.6% | (many diffs) |
| 39 | mouse | 13518 | Dst | dystonin | XM_006495686.3 | 36.7% | 39% | (many diffs) |
| 40 | mouse | 13518 | Dst | dystonin | NM_010081.2 | 36.7% | 38.9% | (many diffs) |
| 41 | mouse | 13518 | Dst | dystonin | XM_006495685.2 | 18.6% | 19.9% | (many diffs) |
| 42 | mouse | 13518 | Dst | dystonin | XM_017314868.1 | 17.7% | 18.2% | (many diffs) |
| 43 | mouse | 13518 | Dst | dystonin | NM_133833.3 | 17.7% | 18.2% | (many diffs) |
| 44 | mouse | 13518 | Dst | dystonin | XM_006495684.3 | 17.3% | 17.8% | (many diffs) |
| 45 | mouse | 13518 | Dst | dystonin | XM_017314811.1 | 17.1% | 17.6% | (many diffs) |
| 46 | mouse | 13518 | Dst | dystonin | XM_006495682.2 | 16.7% | 17.3% | (many diffs) |
| 47 | mouse | 13518 | Dst | dystonin | XM_017314794.1 | 16.6% | 17.2% | (many diffs) |
| 48 | mouse | 13518 | Dst | dystonin | XM_006495680.3 | 13.4% | 14.3% | (many diffs) |
| 49 | mouse | 13518 | Dst | dystonin | XM_017314848.1 | 12.8% | 13.3% | (many diffs) |
| 50 | mouse | 13518 | Dst | dystonin | NM_134448.3 | 12.8% | 13.3% | (many diffs) |
| 51 | mouse | 13518 | Dst | dystonin | XM_006495679.3 | 12.7% | 13.2% | (many diffs) |
| 52 | mouse | 13518 | Dst | dystonin | XM_006495688.3 | 12.7% | 13.1% | (many diffs) |
| 53 | mouse | 13518 | Dst | dystonin | XM_006495678.3 | 12.6% | 13% | (many diffs) |
| 54 | mouse | 13518 | Dst | dystonin | XM_017314750.1 | 12.6% | 13% | (many diffs) |
| 55 | mouse | 13518 | Dst | dystonin | XM_017314741.1 | 12.6% | 13% | (many diffs) |
| 56 | mouse | 13518 | Dst | dystonin | XM_006495674.3 | 12.5% | 12.9% | (many diffs) |
| 57 | mouse | 13518 | Dst | dystonin | XM_017314722.1 | 12.5% | 12.9% | (many diffs) |
| 58 | mouse | 13518 | Dst | dystonin | XM_006495691.3 | 12.5% | 12.9% | (many diffs) |
| 59 | mouse | 13518 | Dst | dystonin | XM_017314716.1 | 12.5% | 12.9% | (many diffs) |
| 60 | mouse | 13518 | Dst | dystonin | XM_017314712.1 | 12.5% | 12.9% | (many diffs) |
| 61 | mouse | 13518 | Dst | dystonin | XM_017314706.1 | 12.5% | 12.9% | (many diffs) |
| 62 | mouse | 13518 | Dst | dystonin | XM_006495669.3 | 12.4% | 12.8% | (many diffs) |
| 63 | mouse | 13518 | Dst | dystonin | XM_017314689.1 | 12.4% | 12.8% | (many diffs) |
| 64 | mouse | 13518 | Dst | dystonin | XM_017314687.1 | 12.4% | 12.8% | (many diffs) |
| 65 | mouse | 13518 | Dst | dystonin | XM_006495690.3 | 12.4% | 12.8% | (many diffs) |
| 66 | mouse | 13518 | Dst | dystonin | XM_017314676.1 | 12.3% | 12.8% | (many diffs) |
| 67 | mouse | 13518 | Dst | dystonin | XM_017314665.1 | 12.3% | 12.7% | (many diffs) |
| 68 | mouse | 13518 | Dst | dystonin | XM_017314651.1 | 12.3% | 12.7% | (many diffs) |
| 69 | mouse | 13518 | Dst | dystonin | XM_006495666.3 | 12.3% | 12.7% | (many diffs) |
| 70 | mouse | 13518 | Dst | dystonin | NM_001276764.1 | 12.3% | 12.7% | (many diffs) |
| 71 | mouse | 13518 | Dst | dystonin | XM_017314635.1 | 12.3% | 12.7% | (many diffs) |
| 72 | mouse | 13518 | Dst | dystonin | XM_017314621.1 | 12.3% | 12.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3495
- ORF length:
- 3429
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca cagtagtagt tatagttacc gtagcagtga ttctgtgttt agtaacacta 121 ccagcactcg aaccagtctt gattcaaatg aaaatcttct cttggttcat tgtggtccaa 181 cactgatcaa ctcttgcatt agcttcggca gtgaatcctt tgatggacac aggttagaaa 241 tgttgcaaca gattgccaac agagttcaga gggacagtgt catctgtgaa gacaaactga 301 ttcttgctgg aaatgctctt cagtctgatt ctaaaagatt agaatcagga gtgcagtttc 361 agaatgaagc agaaattgct gggtatatac ttgaatgtga gaacctttta cgccagcatg 421 taattgatgt acagattctt attgatggaa aatactacca ggcagatcaa ttggtacaga 481 gggttgcaaa actgcgtgac gaaattatgg ccttaaggaa cgaatgttct tctgtgtaca 541 gcaaaggacg catactgaca acagaacaga caaagctcat gatatcagga atcactcaaa 601 gtttaaactc aggatttgca cagaccttac accctagtct gacctcaggg ctgacccaga 661 gtttaacacc ttccctaacc tcttctagta tgacttctgg cctgtcatca gggatgactt 721 cccgcctgac tccatctgtc actccagctt atacacctgg tttcccatca ggattagttc 781 caaatttcag ttcaggagta gagccaaatt cattgcaaac tttgaagttg atgcagatcc 841 gaaaacccct tctaaagtct tctttgctgg atcaaaattt aacagaagaa gaaatcaata 901 tgaaatttgt tcaggatctt ttgaattggg ttgatgagat gcaggtacaa ctggaccgca 961 ctgagtgggg ctcagatttg ccaagtgttg aaagccattt agaaaatcat aaaaatgttc 1021 atagagctat tgaagaattt gaatctagtc tcaaagaagc taaaatcagt gagattcaaa 1081 tgacagcacc tcttaaactg acttatgcag aaaagttgca cagattagag agtcagtatg 1141 caaaactctt gaatacatcc aggaatcaag aacggcacct tgatacactc cataattttg 1201 taagtcgtgc gactaatgaa cttatttggt tgaatgaaaa agaagaggag gaagttgctt 1261 atgactggag tgagagaaac accaacatag ctaggaaaaa agattatcat gctgaattaa 1321 tgagagaact tgatcaaaag gaagaaaata ttaaatcagt tcaggagata gcagagcagc 1381 tacttctaga aaatcatcca gcccggttaa ctattgaggc ctacagagcg gcaatgcaga 1441 cgcagtggag ctggatctta cagctctgcc agtgtgtgga gcagcacata aaggagaaca 1501 cagcgtattt cgagtttttc aatgatgcca aagaagctac tgattactta aggaatctaa 1561 aagatgccat tcagcggaag tacagctgtg atagatcaag cagcattcac aagctagaag 1621 accttgttca ggaatcaatg gaagagaaag aagaacttct gcagtacaaa agcactatag 1681 caaacctaat gggaaaagca aaaacaataa ttcaactgaa gccaaggaat tctgactgtc 1741 cactcaaaac ttctattccg atcaaagcta tctgtgacta cagacaaatt gagataacca 1801 tttacaaaga cgatgaatgt gttttggcga ataactctca tcgtgctaaa tggaaggtca 1861 ttagtcctac tgggaatgag gctatggtcc catctgtgtg cttcaccgtt cctccaccaa 1921 acaaagaagc ggtggacctt gccaacagaa ttgagcaaca gtatcagaat gtcctgactc 1981 tttggcatga gtctcacata aacatgaaga gtgtagtatc ctggcattat ctcatcaatg 2041 aaattgatag aattcgagct agcaatgtgg cttcaataaa gacaatgcta cctggtgaac 2101 atcagcaagt tctaagtaat ctacaatctc gttttgaaga ttttctggaa gatagccagg 2161 aatcccaagt cttttcaggc tcagatataa cacaactgga aaaggaggtt aatgtatgta 2221 agcagtatta tcaagaactt cttaaatctg cagaaagaga ggagcaagag gaatcagttt 2281 ataatctcta catctctgaa gttcgaaaca ttagacttcg gttagagaac tgtgaagatc 2341 ggctgattag acagattcga actcccctgg aaagagatga tttgcatgaa agtgtgttca 2401 gaatcacaga acaggagaaa ctaaagaaag agctggaacg acttaaagat gatttgggaa 2461 caatcacaaa taagtgtgag gagtttttca gtcaagcagc agcctcttca tcagtcccta 2521 ccctacgatc agagcttaat gtggtccttc agaacatgaa ccaagtctat tctatgtctt 2581 ccacttacat agataagttg aaaactgtta acttggtgtt aaaaaacact caagctgcag 2641 aagccctcgt aaaactctat gaaactaaac tgtgtgaaga agaagcagtt atagctgaca 2701 agaataatat tgagaatcta ataagtactt taaagcaatg gagatctgaa gtagatgaaa 2761 agagacaggt attccatgcc ttagaggatg agttgcagaa agctaaagcc atcagtgatg 2821 aaatgtttaa aacgtataaa gaacgggacc ttgattttga ctggcacaaa gaaaaagcag 2881 atcaattagt tgaaaggtgg caaaatgttc atgtgcagat tgacaacagg ttacgggact 2941 tagagggcat tggcaaatca ctgaagtact acagagacac ttaccatcct ttagatgatt 3001 ggatccagca ggttgaaact actcagagaa agattcagga aaatcagcct gaaaatagta 3061 aaaccctagc cacacagttg aatcaacaga agatgctggt gtccgaaata gaaatgaaac 3121 agagcaaaat ggacgagtgt caaaaatatg cagaacagta cTCAGCTACA GTGAAGGACT 3181 ATGAATTACA AACAATGACC TACCGGGCCA TGGTAGATTC ACAACAAAAA TCTCCAGTGA 3241 AACGCCGAAG AATGCAGAGT TCAGCAGATC TCATTATTCA AGAGTTCATG GACCTAAGGA 3301 CTCGATATAC TGCCCTGGTC ACTCTCATGA CACAATATAT TAAATTTGCT GGTGATTCAT 3361 TGAAGAGGCT GGAAGAGGAG GAGGTAATCA TTAACAGTGA GCACCCTGTA AATATAAAGG 3421 AGCTAGAGAT GAGCAGTAAC TACAGTGTAG TTTGGGAAAG CAAAACATGT TTCATGATAA 3481 ATTTATGTGG CCGGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3541 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3601 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTATCGAGCT CTGCGTGTCA 3661 CTGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt