Transcript: Mouse XM_006495680.3

PREDICTED: Mus musculus dystonin (Dst), transcript variant X19, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dst (13518)
Length:
22873
CDS:
165..21809

Additional Resources:

NCBI RefSeq record:
XM_006495680.3
NBCI Gene record:
Dst (13518)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006495680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000377035 ACTCTTATGACGCAGTATATT pLKO_005 3420 CDS 100% 15.000 21.000 N Dst n/a
2 TRCN0000377036 GCGATGCTCTAACTGTAAATT pLKO_005 22243 3UTR 100% 15.000 21.000 N Dst n/a
3 TRCN0000366702 GGTCGTCAAAGCAGCATAAAT pLKO_005 11607 CDS 100% 15.000 21.000 N Dst n/a
4 TRCN0000088313 CGGATATAAGTCAACTAGAAA pLKO.1 2281 CDS 100% 5.625 7.875 N Dst n/a
5 TRCN0000088315 GCCACTGATTACCTAAGGAAT pLKO.1 1635 CDS 100% 4.950 6.930 N Dst n/a
6 TRCN0000376794 TAGTAATGTGGCTTCGATAAA pLKO_005 2159 CDS 100% 13.200 10.560 N Dst n/a
7 TRCN0000088314 CCCTTGTCAAACTCTACGAAA pLKO.1 2743 CDS 100% 4.950 3.960 N Dst n/a
8 TRCN0000088316 GCCCTTGTCAAACTCTACGAA pLKO.1 2742 CDS 100% 3.000 2.400 N Dst n/a
9 TRCN0000366629 TATTCTCAGGCTCGGATATAA pLKO_005 2269 CDS 100% 15.000 10.500 N Dst n/a
10 TRCN0000377037 AGTTGAACAGATCGGTGATAA pLKO_005 20870 CDS 100% 13.200 9.240 N Dst n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006495680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10698 pDONR223 100% 13.4% 14.3% None (many diffs) n/a
2 ccsbBroad304_10698 pLX_304 0% 13.4% 14.3% V5 (many diffs) n/a
3 TRCN0000467550 GTATCGAGCTCTGCGTGTCACTGC pLX_317 9.9% 13.4% 14.3% V5 (many diffs) n/a
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