Construct: ORF TRCN0000467575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012437.1_s317c1
- Derived from:
- ccsbBroadEn_05158
- DNA Barcode:
- TTGCTGCAGTTATCGCAACGATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XAGE3 (170626)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 170626 | XAGE3 | X antigen family member 3 | NM_130776.1 | 100% | 100% | |
| 2 | human | 170626 | XAGE3 | X antigen family member 3 | NM_133179.3 | 100% | 100% | |
| 3 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530768.2 | 100% | 100% | |
| 4 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530769.2 | 100% | 100% | |
| 5 | human | 170626 | XAGE3 | X antigen family member 3 | XM_011530770.2 | 100% | 100% | |
| 6 | human | 139629 | XAGE-4 | XAGE-4 protein | XR_001755743.1 | 89.3% | (many diffs) | |
| 7 | human | 9502 | XAGE2 | X antigen family member 2 | NM_130777.3 | 86.4% | 72% | (many diffs) |
| 8 | human | 9502 | XAGE2 | X antigen family member 2 | XM_011530836.2 | 86.4% | 72% | (many diffs) |
| 9 | human | 170627 | XAGE5 | X antigen family member 5 | NM_130775.2 | 85.9% | 72.9% | (many diffs) |
| 10 | human | 170627 | XAGE5 | X antigen family member 5 | XM_017029320.2 | 85.9% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 423
- ORF length:
- 333
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATTTGGCG AGGAAGATCA ACATATAGGC 121 CTAGGCCGAG GAGAAGTGTA CCACCTCCTG AGCTGATTGG GCCTATGCTG GAGCCCGGTG 181 ATGAGGAGCC TCAGCAAGAG GAACCACCAA CTGAAAGTCG GGATCCTGCA CCTGGTCAGG 241 AGAGAGAAGA AGATCAGGGT GCAGCTGAGA CTCAAGTGCC TGACCTGGAA GCTGATCTCC 301 AGGAGCTGTC TCAGTCAAAG ACTGGGGGTG AATGTGGAAA TGGTCCTGAT GACCAGGGGA 361 AGATTCTGCC AAAATCAGAA CAATTTAAAA TGCCAGAAGG AGGTGACAGG CAACCACAGG 421 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTCTTG 541 GCTTTATATA TCTTGTGGAA AGGACGATTG CTGCAGTTAT CGCAACGATG AACGCGTTAA 601 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t