Construct: ORF TRCN0000467598
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015147.1_s317c1
- Derived from:
- ccsbBroadEn_01162
- DNA Barcode:
- CACACCATAGTACTACCGGCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFB (5155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467598
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5155 | PDGFB | platelet derived growth fac... | NM_002608.4 | 100% | 100% | |
| 2 | human | 5155 | PDGFB | platelet derived growth fac... | NM_033016.3 | 92.8% | 91.2% | (many diffs) |
| 3 | mouse | 18591 | Pdgfb | platelet derived growth fac... | NM_011057.3 | 86% | 89.6% | (many diffs) |
| 4 | mouse | 18591 | Pdgfb | platelet derived growth fac... | XM_017316502.1 | 86% | 89.6% | (many diffs) |
| 5 | mouse | 18591 | Pdgfb | platelet derived growth fac... | XM_006520592.3 | 62.5% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcgctgctgg gcgctcttcc tgtctctctg ctgctacctg cgtctggtca 121 gcgccgaggg ggaccccatt cccgaggagc tttatgagat gctgagtgac cactcgatcc 181 gctcctttga tgatctccaa cgcctgctgc acggagaccc cggagaggaa gatggggccg 241 agttggacct gaacatgacc cgctcccact ctggaggcga gctggagagc ttggctcgtg 301 gaagaaggag cctgggttcc ctgaccattg ctgagccggc catgatcgcc gagtgcaaga 361 cgcgcaccga ggTGTTCGAG ATCTCCCGGC GCCTCATAGA CCGCACCAAC GCCAACTTCC 421 TGGTGTGGCC GCCCTGTGTG GAGGTGCAGC GCTGCTCCGG CTGCTGCAAC AACCGCAACG 481 TGCAGTGCCG CCCCACCCAG GTGCAGCTGC GACCTGTCCA GGTGAGAAAG ATCGAGATTG 541 TGCGGAAGAA GCCAATCTTT AAGAAGGCCA CGGTGACGCT GGAAGACCAC CTGGCATGCA 601 AGTGTGAGAC AGTGGCAGCT GCACGGCCTG TGACCCGAAG CCCGGGGGGT TCCCAGGAGC 661 AGCGAGCCAA AACGCCCCAA ACTCGGGTGA CCATTCGGAC GGTGCGAGTC CGCCGGCCCC 721 CCAAGGGCAA GCACCGGAAA TTCAAGCACA CGCATGACAA GACGGCACTG AAGGAGACCC 781 TTGGAGCCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACACACC ATAGTACTAC CGGCTATCAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt