Construct: ORF TRCN0000467602

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001892.1_s317c1
Derived from:
ccsbBroadEn_01202
DNA Barcode:
TCTTCGCAGCGGTTACTCCATTGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PIK3CB (5291)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467602
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5291 PIK3CB phosphatidylinositol-4,5-bi... NM_006219.3 100% 100%
2 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_006713659.3 100% 100%
3 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_011512895.2 100% 100%
4 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_017006619.1 100% 100%
5 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_024453596.1 93% 93% 0_1ins171;1359_1360ins51
6 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XR_002959542.1 64.4% 0_1ins155;1879_1880ins100;2956_4331del
7 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_024453597.1 62.9% 62.9% 0_1ins1188
8 human 5291 PIK3CB phosphatidylinositol-4,5-bi... XM_017006621.2 61.4% 61.4% 0_1ins1188;342_343ins51
9 human 5291 PIK3CB phosphatidylinositol-4,5-bi... NM_001256045.1 54.3% 54.3% 0_1ins1413;117_118ins51
10 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... NM_029094.3 87.9% 95.3% (many diffs)
11 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_011242829.2 87.9% 95.3% (many diffs)
12 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_011242830.2 87.9% 95.3% (many diffs)
13 mouse 74769 Pik3cb phosphatidylinositol-4,5-bi... XM_006511520.2 75.6% 82.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3279
ORF length:
3210
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtgcttcagt ttcataatgc ctcctgctat ggcagacatc cttgacatct
121gggcggtgga ttcacagata gcatctgatg gctccatacc tgtggatttc cttttgccca
181ctgggattta tatccagttg gaggtacctc gggaagctac catttcttat attaagcaga
241tgttatggaa gcaagttcac aattacccaa tgttcaacct ccttatggat attgactcct
301atatgtttgc atgtgtgaat cagactgctg tatatgagga gcttgaagat gaaacacgaa
361gactctgtga tgtcagacct tttcttccag ttctcaaatt agtgacaaga agttgtgacc
421caggggaaaa attagactca aaaattggag tccttatagg aaaaggtctg catgaatttg
481attccttgaa ggatcctgaa gtaaatgaat ttcgaagaaa aatgcgcaaa ttcagcgagg
541aaaaaatcct gtcacttgtg ggattgtctt ggatggactg gctaaaacaa acatatccac
601cagagcatga accatccatc cctgaaaact tagaagataa actttatggg ggaaagctca
661tcgtagctgt tcattttgaa aactgccagg acgtgtttag ctttcaagtg tctcctaata
721tgaatcctat caaagtaaat gaattggcaa tccaaaaacg tttgactatt catgggaagg
781aagatgaagt tagcccctat gattatgtgt tgcaagtcag cgggagagta gaatatgttt
841ttggtgatca tccactaatt cagttccagt atatccggaa ctgtgtgatg aacagagccc
901tgccccattt tatacttgtg gaatgctgca agatcaagaa aatgtatgaa caagaaatga
961ttgccataga ggctgccata aatcgaaatt catctaatct tcctcttcca ttaccaccaa
1021agaaaacacg aattatttct catgtttggg aaaataacaa ccctttccaa attgtcttgg
1081ttaagggaaa taaacttaac acagaggaaa ctgtaaaagt tcatgtcagg gctggtcttt
1141ttcatggtac tgagctcctg tgtaaaacca tcgtaagctc agaggtatca gggaaaaatg
1201atcatatttg gaatgaacca ctggaatttg atattaatat ttgtgactta ccaagaatgg
1261ctcgattatg ttttgctgtt tatgcagttt tggataaagt aaaaacgaag aaatcaacga
1321aaactattaa tccctctaaa tatcagacca tcaggaaagc tggaaaagtg cattatcctg
1381tagcgtgggt aaatacgatg gtttttgact ttaaaggaca attgagaact ggagacataa
1441tattacacag ctggtcttca tttcctgatg aactcgaaga aatgttgaat ccaatgggaa
1501ctgttcaaac aaatccatat actgaaaatg caacagcttt gcatgttaaa tttccagaga
1561ataaaaaaca accttattat taccctccct tcgataagat tattgaaaag gcagctgaga
1621ttgcaagcag tgatagtgct aatgtgtcaa gtcgaggtgg aaaaaagttt cttcctgtat
1681tgaaagaaat cttggacagg gatcccttgt ctcaactgtg tgaaaatgaa atggatctta
1741tttggacttt gcgacaagac tgccgagaga ttttcccaca atcactgcca aaattactgc
1801tgtcaatcaa gtggaataaa cttgaggatg ttgctcagct tcaggcgctg cttcagattt
1861ggcctaaact gcccccccgg gaggccctag agcttctgga tttcaactat ccagaccagt
1921acgttcgaga atatgctgta ggctgcctgc gacagatgag tgatgaagaa ctttctcaat
1981atcttttaca actggtgcaa gtgttaaaat atgagccttt tcttgattgt gccctctcta
2041gattcctatt agaaagagca cttggtaatc ggaggatagg gcagtttcta ttttggcatc
2101ttaggtcaga agtgcacatt cctgctgtct cagtacaatt tggtgtcatc cttgaagcat
2161actgccgggg aagtgtgggg cacatgaaag tgctttctaa gcaggttgaa gcactcaata
2221agttaaaaac tttaaatagt ttaatcaaac tgaatgccgt gaagttaaac agagccaaag
2281ggaaggaggc catgcatacc tgtttaaaac agagtgctta ccgggaagcc ctctctgacc
2341tgcagtcacc cctgaaccca tgtgttatcc tctcagaact ctatgttgaa aagtgcaaat
2401acatggattc caaaatgaag cctttgtggc tggtatacaa taacaaggta tttggtgagg
2461attcagttgg agtgattttt aaaaatggtg atgatttacg acaggatatg ttgacactcc
2521aaatgttgcg cttgatggat ttactctgga aagaagctgg tttggatctt cggatgttgc
2581cttatggctg tttagcaaca ggagatcgct ctggcctcat tgaagttgtg agcacctctg
2641aaacaattgc tgacattcag ctgaacagta gcaatgtggc tgctgcagca gccttcaaca
2701aagatgccct tctgaactgg cttaaagaat acaactctgg ggatgacctg gaccgagcca
2761ttgaggaatt tacactgtcc tgtgctggct actgtgtagc ttcttatgtc cttgggattG
2821GTGACAGACA TAGTGACAAC ATCATGGTCA AAAAAACTGG CCAGCTCTTC CACATTGACT
2881TTGGACATAT TCTTGGAAAT TTCAAATCTA AGTTTGGCAT TAAAAGGGAG CGAGTGCCTT
2941TTATTCTTAC CTATGATTTC ATCCATGTCA TTCAACAAGG AAAAACAGGA AATACAGAAA
3001AGTTTGGCCG GTTCCGCCAG TGTTGTGAGG ATGCATATCT GATTTTACGA CGGCATGGGA
3061ATCTCTTCAT CACTCTCTTT GCGCTGATGT TGACTGCAGG GCTTCCTGAA CTCACATCAG
3121TCAAAGATAT ACAGTATCTT AAGGACTCTC TTGCATTAGG GAAGAGTGAA GAAGAAGCAC
3181TCAAACAGTT TAAGCAAAAA TTTGATGAGG CGCTCAGGGA AAGCTGGACT ACTAAAGTGA
3241ACTGGATGGC CCACACAGTT CGGAAAGACT ACAGATCTTT GCCAACTTTC TTGTACAAAG
3301TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
3361GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
3421ACGATCTTCG CAGCGGTTAC TCCATTGCAC GCGTTAAGTC gacaatcaac ctctggatta
3481caaaatttgt gaaagatt

Download FASTA (ORF) (Full)