Construct: ORF TRCN0000467602
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001892.1_s317c1
- Derived from:
- ccsbBroadEn_01202
- DNA Barcode:
- TCTTCGCAGCGGTTACTCCATTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3CB (5291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467602
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_006219.3 | 100% | 100% | |
| 2 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_006713659.3 | 100% | 100% | |
| 3 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_011512895.2 | 100% | 100% | |
| 4 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006619.1 | 100% | 100% | |
| 5 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453596.1 | 93% | 93% | 0_1ins171;1359_1360ins51 |
| 6 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XR_002959542.1 | 64.4% | 0_1ins155;1879_1880ins100;2956_4331del | |
| 7 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_024453597.1 | 62.9% | 62.9% | 0_1ins1188 |
| 8 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | XM_017006621.2 | 61.4% | 61.4% | 0_1ins1188;342_343ins51 |
| 9 | human | 5291 | PIK3CB | phosphatidylinositol-4,5-bi... | NM_001256045.1 | 54.3% | 54.3% | 0_1ins1413;117_118ins51 |
| 10 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | NM_029094.3 | 87.9% | 95.3% | (many diffs) |
| 11 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242829.2 | 87.9% | 95.3% | (many diffs) |
| 12 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_011242830.2 | 87.9% | 95.3% | (many diffs) |
| 13 | mouse | 74769 | Pik3cb | phosphatidylinositol-4,5-bi... | XM_006511520.2 | 75.6% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3279
- ORF length:
- 3210
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcttcagt ttcataatgc ctcctgctat ggcagacatc cttgacatct 121 gggcggtgga ttcacagata gcatctgatg gctccatacc tgtggatttc cttttgccca 181 ctgggattta tatccagttg gaggtacctc gggaagctac catttcttat attaagcaga 241 tgttatggaa gcaagttcac aattacccaa tgttcaacct ccttatggat attgactcct 301 atatgtttgc atgtgtgaat cagactgctg tatatgagga gcttgaagat gaaacacgaa 361 gactctgtga tgtcagacct tttcttccag ttctcaaatt agtgacaaga agttgtgacc 421 caggggaaaa attagactca aaaattggag tccttatagg aaaaggtctg catgaatttg 481 attccttgaa ggatcctgaa gtaaatgaat ttcgaagaaa aatgcgcaaa ttcagcgagg 541 aaaaaatcct gtcacttgtg ggattgtctt ggatggactg gctaaaacaa acatatccac 601 cagagcatga accatccatc cctgaaaact tagaagataa actttatggg ggaaagctca 661 tcgtagctgt tcattttgaa aactgccagg acgtgtttag ctttcaagtg tctcctaata 721 tgaatcctat caaagtaaat gaattggcaa tccaaaaacg tttgactatt catgggaagg 781 aagatgaagt tagcccctat gattatgtgt tgcaagtcag cgggagagta gaatatgttt 841 ttggtgatca tccactaatt cagttccagt atatccggaa ctgtgtgatg aacagagccc 901 tgccccattt tatacttgtg gaatgctgca agatcaagaa aatgtatgaa caagaaatga 961 ttgccataga ggctgccata aatcgaaatt catctaatct tcctcttcca ttaccaccaa 1021 agaaaacacg aattatttct catgtttggg aaaataacaa ccctttccaa attgtcttgg 1081 ttaagggaaa taaacttaac acagaggaaa ctgtaaaagt tcatgtcagg gctggtcttt 1141 ttcatggtac tgagctcctg tgtaaaacca tcgtaagctc agaggtatca gggaaaaatg 1201 atcatatttg gaatgaacca ctggaatttg atattaatat ttgtgactta ccaagaatgg 1261 ctcgattatg ttttgctgtt tatgcagttt tggataaagt aaaaacgaag aaatcaacga 1321 aaactattaa tccctctaaa tatcagacca tcaggaaagc tggaaaagtg cattatcctg 1381 tagcgtgggt aaatacgatg gtttttgact ttaaaggaca attgagaact ggagacataa 1441 tattacacag ctggtcttca tttcctgatg aactcgaaga aatgttgaat ccaatgggaa 1501 ctgttcaaac aaatccatat actgaaaatg caacagcttt gcatgttaaa tttccagaga 1561 ataaaaaaca accttattat taccctccct tcgataagat tattgaaaag gcagctgaga 1621 ttgcaagcag tgatagtgct aatgtgtcaa gtcgaggtgg aaaaaagttt cttcctgtat 1681 tgaaagaaat cttggacagg gatcccttgt ctcaactgtg tgaaaatgaa atggatctta 1741 tttggacttt gcgacaagac tgccgagaga ttttcccaca atcactgcca aaattactgc 1801 tgtcaatcaa gtggaataaa cttgaggatg ttgctcagct tcaggcgctg cttcagattt 1861 ggcctaaact gcccccccgg gaggccctag agcttctgga tttcaactat ccagaccagt 1921 acgttcgaga atatgctgta ggctgcctgc gacagatgag tgatgaagaa ctttctcaat 1981 atcttttaca actggtgcaa gtgttaaaat atgagccttt tcttgattgt gccctctcta 2041 gattcctatt agaaagagca cttggtaatc ggaggatagg gcagtttcta ttttggcatc 2101 ttaggtcaga agtgcacatt cctgctgtct cagtacaatt tggtgtcatc cttgaagcat 2161 actgccgggg aagtgtgggg cacatgaaag tgctttctaa gcaggttgaa gcactcaata 2221 agttaaaaac tttaaatagt ttaatcaaac tgaatgccgt gaagttaaac agagccaaag 2281 ggaaggaggc catgcatacc tgtttaaaac agagtgctta ccgggaagcc ctctctgacc 2341 tgcagtcacc cctgaaccca tgtgttatcc tctcagaact ctatgttgaa aagtgcaaat 2401 acatggattc caaaatgaag cctttgtggc tggtatacaa taacaaggta tttggtgagg 2461 attcagttgg agtgattttt aaaaatggtg atgatttacg acaggatatg ttgacactcc 2521 aaatgttgcg cttgatggat ttactctgga aagaagctgg tttggatctt cggatgttgc 2581 cttatggctg tttagcaaca ggagatcgct ctggcctcat tgaagttgtg agcacctctg 2641 aaacaattgc tgacattcag ctgaacagta gcaatgtggc tgctgcagca gccttcaaca 2701 aagatgccct tctgaactgg cttaaagaat acaactctgg ggatgacctg gaccgagcca 2761 ttgaggaatt tacactgtcc tgtgctggct actgtgtagc ttcttatgtc cttgggattG 2821 GTGACAGACA TAGTGACAAC ATCATGGTCA AAAAAACTGG CCAGCTCTTC CACATTGACT 2881 TTGGACATAT TCTTGGAAAT TTCAAATCTA AGTTTGGCAT TAAAAGGGAG CGAGTGCCTT 2941 TTATTCTTAC CTATGATTTC ATCCATGTCA TTCAACAAGG AAAAACAGGA AATACAGAAA 3001 AGTTTGGCCG GTTCCGCCAG TGTTGTGAGG ATGCATATCT GATTTTACGA CGGCATGGGA 3061 ATCTCTTCAT CACTCTCTTT GCGCTGATGT TGACTGCAGG GCTTCCTGAA CTCACATCAG 3121 TCAAAGATAT ACAGTATCTT AAGGACTCTC TTGCATTAGG GAAGAGTGAA GAAGAAGCAC 3181 TCAAACAGTT TAAGCAAAAA TTTGATGAGG CGCTCAGGGA AAGCTGGACT ACTAAAGTGA 3241 ACTGGATGGC CCACACAGTT CGGAAAGACT ACAGATCTTT GCCAACTTTC TTGTACAAAG 3301 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3361 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3421 ACGATCTTCG CAGCGGTTAC TCCATTGCAC GCGTTAAGTC gacaatcaac ctctggatta 3481 caaaatttgt gaaagatt