Construct: ORF TRCN0000467611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008439.1_s317c1
- Derived from:
- ccsbBroadEn_11319
- DNA Barcode:
- TTTCGTAGGATCGCGCTAAGCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERC3 (8916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8916 | HERC3 | HECT and RLD domain contain... | NM_001318505.2 | 100% | 100% | |
| 2 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_005263332.4 | 40.5% | 39.8% | (many diffs) |
| 3 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_005263331.2 | 40.2% | 39.5% | (many diffs) |
| 4 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_005263330.4 | 36.6% | 35.9% | (many diffs) |
| 5 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_005263329.4 | 34.9% | 34.2% | (many diffs) |
| 6 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_017008806.2 | 34.9% | 34.2% | (many diffs) |
| 7 | human | 8916 | HERC3 | HECT and RLD domain contain... | NM_014606.3 | 34.6% | 34% | (many diffs) |
| 8 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_005263327.3 | 34.6% | 34% | (many diffs) |
| 9 | human | 8916 | HERC3 | HECT and RLD domain contain... | XM_017008807.1 | 23.6% | 22.9% | (many diffs) |
| 10 | human | 8916 | HERC3 | HECT and RLD domain contain... | NM_001271602.1 | 23.4% | 22.7% | (many diffs) |
| 11 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XM_006506701.4 | 50.1% | 53.6% | (many diffs) |
| 12 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_001785178.2 | 48.8% | (many diffs) | |
| 13 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_001785177.2 | 45.5% | (many diffs) | |
| 14 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_003956257.1 | 42.3% | (many diffs) | |
| 15 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_001785176.2 | 40% | (many diffs) | |
| 16 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_377496.3 | 39.6% | (many diffs) | |
| 17 | mouse | 73998 | Herc3 | hect domain and RLD 3 | NM_001361967.1 | 35.4% | 37.8% | (many diffs) |
| 18 | mouse | 73998 | Herc3 | hect domain and RLD 3 | NM_001361966.1 | 30.8% | 32.9% | (many diffs) |
| 19 | mouse | 73998 | Herc3 | hect domain and RLD 3 | NM_028705.3 | 30.8% | 32.9% | (many diffs) |
| 20 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XM_006506696.3 | 30.8% | 32.9% | (many diffs) |
| 21 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XM_006506697.4 | 30.8% | 32.9% | (many diffs) |
| 22 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XM_006506698.4 | 30.8% | 32.9% | (many diffs) |
| 23 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XM_030255634.1 | 30.8% | 32.9% | (many diffs) |
| 24 | mouse | 73998 | Herc3 | hect domain and RLD 3 | XR_377495.3 | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1170
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt atgttgggga tattggtctc tgggccaacc tggtatcagc accaacctgc 121 agggaattgt ggctgagccc caggtgtgtg ggttcatatc tgacagaagt gtcaaggaag 181 tggcctgtgg gggaaaccac tctgtgttcc tgctggaaga tggggaagtt tacacatgtg 241 gtttgaacac caaggggcaa ctgggccatg agagggaagg aaacaagcca gaacaaattg 301 gagctctggc agatcagcat atcattcatg tggcatgtgg cgagtcccac agtctggccc 361 tcagtgaccg aggccagctg ttttcttggg gtgcagggag tgatggtcag ctaggactca 421 tgactactga ggattctgtg gcagtgccca ggttaataca aaagctgaac cagcaaacaa 481 tattacaagt ttcctgtggc aactggcatt gcttggctct tgcggctgat ggccagttct 541 tcacctgggg aaagaacagc catgggcagc ttggcttagg gaaggagttc ccctcccaag 601 ccagcccaca gagggtgagg tccctggagg ggatcccact ggctcaggtg gctgccggag 661 gggctcacag ctttgccctg tctctctcag gagctgtttt tggctggggg atgaataatg 721 ccgggcagcT AGGGCTCAGT GATGAAAAAG ATCGAGAATC TCCATGCCAT GTAAAACTCT 781 TACGCACGCA AAAAGTTGTC TATATTAGTT GTGGAGAAGA ACACACAGCA GTTCTCACAA 841 AGAGTGGAGG TGTGTTTACC TTTGGCGCTG GTTCCTGTGG GCAACTTGGA CACGACTCCA 901 TGAATGATGA GGTTAACCCT AGAAGAGTTC TAGAGCTGAT GGGTAGTGAA GTAACTCAAA 961 TTGCTTGTGG CAGACAACAT ACCCTAGCCT TCGTGCCTTC TTCTGGACTC ATCTATGCAT 1021 TTGGTTGTGG AGCAAGAGGT CAATTAGGAA CTGGGCACAC TTGTAATGTT AAGTGCCCAT 1081 CTCCTGTCAA GGGTTACTGG GCTGCCCACA GTGGCCAGCT TTCAGCCCGA GCTGGTAAGA 1141 ATGATTGTCT CTGGAATTTA AAGGTATTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1201 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1261 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTCG 1321 TAGGATCGCG CTAAGCAAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1381 tgaaagatt