Transcript: Human XM_005263327.3

PREDICTED: Homo sapiens HECT and RLD domain containing E3 ubiquitin protein ligase 3 (HERC3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HERC3 (8916)
Length:
5862
CDS:
367..3519

Additional Resources:

NCBI RefSeq record:
XM_005263327.3
NBCI Gene record:
HERC3 (8916)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005263327.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000428511 GCCCGAGCTGATCGCTTTAAA pLKO_005 1426 CDS 100% 15.000 12.000 N HERC3 n/a
2 TRCN0000432539 TGTGGAGCAAGAGGTCAATTA pLKO_005 1327 CDS 100% 13.200 9.240 N HERC3 n/a
3 TRCN0000000292 AGGGTGTTATTTGAGAAGTTA pLKO.1 1756 CDS 100% 5.625 3.938 N HERC3 n/a
4 TRCN0000000293 CCAAGCACATTATACCAGTTT pLKO.1 1548 CDS 100% 4.950 3.465 N HERC3 n/a
5 TRCN0000000295 CGAGAAAGCTATGGAGTGATT pLKO.1 2947 CDS 100% 4.950 3.465 N HERC3 n/a
6 TRCN0000000294 CAAGGATAACAGGCAGGAATT pLKO.1 3012 CDS 100% 0.000 0.000 N HERC3 n/a
7 TRCN0000000291 GATGTGTTTCTGGGATTGTAT pLKO.1 3654 3UTR 100% 5.625 3.375 N HERC3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005263327.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11319 pDONR223 100% 34.6% 34% None (many diffs) n/a
2 ccsbBroad304_11319 pLX_304 0% 34.6% 34% V5 (many diffs) n/a
3 TRCN0000467611 TTTCGTAGGATCGCGCTAAGCAAG pLX_317 40.1% 34.6% 34% V5 (many diffs) n/a
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