Construct: ORF TRCN0000467653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013939.1_s317c1
- Derived from:
- ccsbBroadEn_06049
- DNA Barcode:
- TACACTGTCTTATATTTCATTCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CSF1 (1435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467653
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1435 | CSF1 | colony stimulating factor 1 | NM_000757.6 | 99.8% | 99.8% | 1095C>A;1466T>C;1653A>G |
| 2 | human | 1435 | CSF1 | colony stimulating factor 1 | NM_172212.3 | 99.8% | 99.8% | 1095C>A;1466T>C;1653A>G |
| 3 | human | 1435 | CSF1 | colony stimulating factor 1 | XM_017000369.1 | 92.4% | 92.4% | (many diffs) |
| 4 | human | 1435 | CSF1 | colony stimulating factor 1 | NM_172210.3 | 78.9% | 78.8% | 1089_1090ins348;1118T>C;1305A>G |
| 5 | human | 1435 | CSF1 | colony stimulating factor 1 | NM_172211.4 | 46% | 46% | 542_543ins894;572T>C;759A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1728
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgcgccgggc gccgccgggc gctgccctcc cacgacatgg ctgggctccc 121 tgctgttgtt ggtctgtctc ctggcgagca ggagtatcac cgaggaggtg tcggagtact 181 gtagccacat gattgggagt ggacacctgc agtctctgca gcggctgatt gacagtcaga 241 tggagacctc gtgccaaatt acatttgagt ttgtagacca ggaacagttg aaagatccag 301 tgtgctacct taagaaggca tttctcctgg tacaagacat aatggaggac accatgcgct 361 tcagagataa cacccccaat gccatcgcca ttgtgcagct gcaggaactc tctttgaggc 421 tgaagagctg cttcaccaag gattatgaag agcatgacaa ggcctgcgtc cgaactttct 481 atgagacacc tctccagttg ctggagaagg tcaagaatgt ctttaatgaa acaaagaatc 541 tccttgacaa ggactggaat attttcagca agaactgcaa caacagcttt gctgaatgct 601 ccagccaaga tgtggtgacc aagcctgatt gcaactgcct gtaccccaaa gccatcccta 661 gcagtgaccc ggcctctgtc tcccctcatc agcccctcgc cccctccatg gcccctgtgg 721 ctggcttgac ctgggaggac tctgagggaa ctgagggcag ctccctcttg cctggtgagc 781 agcccctgca cacagtggat ccaggcagtg ccaagcagcg gccacccagg agcacctgcc 841 agagctttga gccgccagag accccagttg tcaaggacag caccatcggt ggctcaccac 901 agcctcgccc ctctgtcggg gccttcaacc ccgggatgga ggatattctt gactctgcaa 961 tgggcactaa ttgggtccca gaagaagcct ctggagaggc cagtgagatt cccgtacccc 1021 aagggacaga gctttccccc tccaggccag gagggggcag catgcagaca gagcccgcca 1081 gacccagcaa cttcctctca gcatcttctc cactccctgc atcagcaaag ggccaacagc 1141 cggcagatgt aactggtaca gccttgccca gggtgggccc cgtgaggccc actggccagg 1201 actggaatca caccccccag aagacagacc atccatctgc cctgctcaga gaccccccgg 1261 agccaggctc tcccaggatc tcatcactgc gcccccaggg cctcagcaac ccctccaccc 1321 tctctgcTCA GCCACAGCTT TCCAGAAGCC ACTCCTCGGG CAGCGTGCTG CCCCTTGGGG 1381 AGCTGGAGGG CAGGAGGAGC ACCAGGGATC GGAGGAGCCC CGCAGAGCCA GAAGGAGGAC 1441 CAGCAAGTGA AGGGGCAGCC AGGCCCCTGC CCCGTTTTAA CTCCGTTCCT TTGACTGACA 1501 CAGGCCATGA GAGGCAGTCC GAGGGATCCT CCAGCCCGCA GCTCCAGGAG TCTGTCTTCC 1561 ACCTGCTGGT GCCCAGTGTC ATCCTGGTCT TGCTGGCCGT CGGAGGCCTC TTGTTCTACA 1621 GGTGGAGGCG GCGGAGCCAT CAAGAGCCTC AGAGAGCGGA TTCTCCCTTG GAGCAACCAG 1681 AGGGCAGCCC CCTGACTCAG GATGACAGAC AGGTGGAGCT GCCAGTGTAA CCAACTTTCT 1741 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1801 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1861 GTGGAAAGGA CGATACACTG TCTTATATTT CATTCTTACG CGTTAAGTCg acaatcaacc 1921 tctggattac aaaatttgtg aaagatt