Transcript: Human NM_172210.3

Homo sapiens colony stimulating factor 1 (CSF1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-03
Taxon:
Homo sapiens (human)
Gene:
CSF1 (1435)
Length:
1505
CDS:
176..1492

Additional Resources:

NCBI RefSeq record:
NM_172210.3
NBCI Gene record:
CSF1 (1435)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_172210.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000282422 TCTCCTGGTACAAGACATAAT pLKO_005 433 CDS 100% 13.200 18.480 N CSF1 n/a
2 TRCN0000262773 AGATCCAGTGTGCTACCTTAA pLKO_005 403 CDS 100% 10.800 15.120 N CSF1 n/a
3 TRCN0000262776 TTGACAAGGACTGGAATATTT pLKO_005 654 CDS 100% 15.000 10.500 N CSF1 n/a
4 TRCN0000262775 AGTACTGTAGCCACATGATTG pLKO_005 285 CDS 100% 10.800 7.560 N CSF1 n/a
5 TRCN0000058559 CGTGCCAAATTACATTTGAGT pLKO.1 360 CDS 100% 3.000 2.100 N CSF1 n/a
6 TRCN0000058561 GCGTCCGAACTTTCTATGAGA pLKO.1 576 CDS 100% 3.000 2.100 N CSF1 n/a
7 TRCN0000058560 AGGATATTCTTGACTCTGCAA pLKO.1 1050 CDS 100% 2.640 1.848 N CSF1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172210.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06049 pDONR223 100% 78.9% 78.8% None 1089_1090ins348;1118T>C;1305A>G n/a
2 ccsbBroad304_06049 pLX_304 0% 78.9% 78.8% V5 (not translated due to prior stop codon) 1089_1090ins348;1118T>C;1305A>G n/a
3 TRCN0000467653 TACACTGTCTTATATTTCATTCTT pLX_317 24.4% 78.9% 78.8% V5 (not translated due to prior stop codon) 1089_1090ins348;1118T>C;1305A>G n/a
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