Construct: ORF TRCN0000467665

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000576.1_s317c1
Derived from:
ccsbBroadEn_11708
DNA Barcode:
TTAGCTTTGATTCAATATCGGCAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCF2L (23263)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467665
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23263 MCF2L MCF.2 cell line derived tra... NM_001320817.2 100% 100%
2 human 23263 MCF2L MCF.2 cell line derived tra... XM_017020499.2 93.6% 92.5% (many diffs)
3 human 23263 MCF2L MCF.2 cell line derived tra... NM_024979.4 87.6% 87.4% 2947_2951delGGTTG;2956_2962delAAAACGT;2965_3369del
4 human 23263 MCF2L MCF.2 cell line derived tra... NM_001112732.3 86.4% 84.5% (many diffs)
5 human 23263 MCF2L MCF.2 cell line derived tra... NM_001320816.2 86% 84.9% (many diffs)
6 human 23263 MCF2L MCF.2 cell line derived tra... NM_001320815.2 85.6% 85.5% 2948_2958del;2961T>C;2964_3444del
7 human 23263 MCF2L MCF.2 cell line derived tra... NM_001366644.2 85.2% 85% (many diffs)
8 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537487.1 83.4% 83.2% 2948_2961del;2965T>C;2967_3537del
9 human 23263 MCF2L MCF.2 cell line derived tra... XM_017020495.1 82.6% 81.4% (many diffs)
10 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537485.1 82.3% 80.6% (many diffs)
11 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537484.1 81.9% 80.9% (many diffs)
12 human 23263 MCF2L MCF.2 cell line derived tra... XM_017020494.1 81.8% 81.1% (many diffs)
13 human 23263 MCF2L MCF.2 cell line derived tra... NM_001366646.2 81.8% 79.2% (many diffs)
14 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537488.1 81.5% 81.2% (many diffs)
15 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537490.1 81.1% 81% (many diffs)
16 human 23263 MCF2L MCF.2 cell line derived tra... XM_017020493.1 80.8% 79.7% (many diffs)
17 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537482.1 80.1% 79% (many diffs)
18 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537483.2 80.1% 79% (many diffs)
19 human 23263 MCF2L MCF.2 cell line derived tra... NM_001366645.2 80% 77.5% (many diffs)
20 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537486.2 78.8% 77.7% (many diffs)
21 human 23263 MCF2L MCF.2 cell line derived tra... XM_011537491.1 77.9% 75.5% (many diffs)
22 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508723.3 81% 79.7% (many diffs)
23 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508721.3 78.7% 81.5% (many diffs)
24 mouse 17207 Mcf2l mcf.2 transforming sequence... NM_001159485.1 74.4% 77.3% (many diffs)
25 mouse 17207 Mcf2l mcf.2 transforming sequence... NM_001159486.1 74.1% 77% (many diffs)
26 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508720.3 74% 76.3% (many diffs)
27 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508719.3 72.9% 74% (many diffs)
28 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508718.3 72.8% 73.9% (many diffs)
29 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_017312595.1 72.4% 74.6% (many diffs)
30 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508715.2 71.6% 74.4% (many diffs)
31 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508716.3 71.3% 73.7% (many diffs)
32 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508714.3 71.3% 72.3% (many diffs)
33 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508717.3 71.1% 73.3% (many diffs)
34 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508712.3 71% 72.3% (many diffs)
35 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508711.3 70.9% 73.4% (many diffs)
36 mouse 17207 Mcf2l mcf.2 transforming sequence... NM_178076.3 70.9% 73.1% (many diffs)
37 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508710.3 70.7% 73.2% (many diffs)
38 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508713.3 70.5% 72.7% (many diffs)
39 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508709.3 69.6% 70.7% (many diffs)
40 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508708.3 69.6% 70.6% (many diffs)
41 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508706.3 69.4% 70.5% (many diffs)
42 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508707.2 69.4% 70.5% (many diffs)
43 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_011242040.1 69.4% 70.5% (many diffs)
44 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_017312593.1 69.4% 70.5% (many diffs)
45 mouse 17207 Mcf2l mcf.2 transforming sequence... XM_006508722.2 60.1% 62.2% (many diffs)
46 mouse 17207 Mcf2l mcf.2 transforming sequence... XR_001778409.1 31.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3018
ORF length:
2952
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ggtgcgccgg ctgtcactgc tgtgccggga cctctgggcg ctgtggctgc
121tgctgaaggc cggcgcagat gaaatcatgc accaggacat cgtcccgctc tgtgctgccg
181acatccagga ccagctaaag aagcgctttg cttacctgtc cggtgggcgg gggcaggacg
241gaagcccggt tatcaccttc cctgactacc cggccttcag cgagattccg gacaaggagt
301tccagaatgt catgacctac ctcaccagca tccccagcct gcaggacgct ggcatcggat
361tcatcctggt gatagaccgg cgacgggaca aatggacctc cgtgaaggcg tccgtcctgc
421gcatcgcagc atctttcccg gcaaacctgc agctcgtcct cgtgcttcgc ccgacgggtt
481ttttccaaag gactctctcc gacatcgctt tcaaattcaa tagagatgac tttaagatga
541aggtgccggt cataatgctg agctccgtac cagacttaca cggttacatc gataagtcgc
601agctgaccga ggacctgggt gggaccctgg actactgcca ctcccggtgg ctgtgccagc
661gcacggccat cgaaagtttc gccctcatgg tgaagcagac ggctcagatg ctgcagtcct
721tcgggaccga gctggctgaa acagagctgc ccaatgacgt ccagtcgaca agctcagtgc
781tgtgtgcgca cacagagaag aaggacaagg cgaaggagga tttgaggctg gcactgaaag
841aggggcacag tgtcctggag agcctcaggg agctgcaggc tgagggctca gagcccagtg
901tgaaccagga ccagcttgac aaccaggcca ccgtgcagag gctcctggcc cagctgaacg
961aaaccgaggc tgccttcgat gagttctggg caaagcatca gcagaaactg gagcagtgtc
1021tgcagctccg gcactttgag cagggcttcc gggaggtcaa agccatcttg gacgcagcgt
1081cccagaagat agcaaccttc acagacatcg gcaacagcct ggcgcatgtg gagcacctgc
1141tgagggacct ggccagcttc gaggagaaat caggcgtggc cgtggagagg gcccgggccc
1201tgtctctgga cggcgagcag ctcattggga acaagcacta cgcggtagac tccatccgcc
1261caaagtgcca ggagctccgg cacctctgtg accagttctc tgcggagatc gcaaggagga
1321gggggctgct cagcaagtcc ctggagctgc accgccgcct ggagacgtcc atgaagtggt
1381gtgatgaagg gatttacctg ctggcctcac aacctgtgga caagtgccag tcccaggacg
1441gcgcggaggc tgccctccag gaaatcgaga agtttttgga gaccggtgcg gaaaataaga
1501tccaggagct caacgcgatt tacaaggaat acgaatccat cctcaaccaa gatctcatgg
1561agcacgtgcg aaaggtcttc cagaagcagg caagcatgga ggaggtgttc caccgcaggc
1621aggccagcct gaagaagctg gcggccaggc agacgcggcc cgtgcagccg gtggccccca
1681gacccgaggc actggcaaag tcgccctgcc cctccccagg cattcggcga ggctctgaga
1741actccagctc cgagggcggt gcgctccgga gagggcccta ccggagggcc aagagtgaga
1801tgagtgagag ccggcagggc cgcggctcag cgggggagga ggaggaaagc ctggccatcc
1861tgcgcaggca cgtgatgagc gagctcctgg acacagaacg ggcctacgtg gaggagctgc
1921tgtgcgtcct ggagggctac gccgcggaga tggataaccc actgatggct cacctcctgt
1981caacaggcct tcacaacaag aaggatgttt tgtttggaaa catggaggaa atctatcact
2041tccacaacag gatattcctc agggagctgg aaaactacac tgactgccca gaactggttg
2101gaagatgctt tctggagagg atggaagatt tccagatcta tgagaagtac tgtcagaaca
2161agccccgctc tgagagcctg tggagacagt gctccgactg cccgtttttc caggaatgcc
2221agagaaagct ggaccacaag ctgagcctgg actcctacct gctgaagcca gtgcagagga
2281tcaccaagta ccagctgctg ctcaaggaaa tgctgaaata cagcaggaac tgcgaggggg
2341ctgaggacct gcaggaggcg ctgagctcca tcctgggcat cctgaaggcc gtgaacgact
2401ccatgcacct catcgctatc accggctatg acgggaatct cggcgacctg ggcaagctgc
2461tgatgcaggg ctcgttcagc gtctggaccg accacaagag gggccacacc aaggtgaagg
2521agctggccag gttcaagccc atgcagcggc acctgttcct gcacgagaag gcagtgctct
2581tctgcaagaa gagggaggag aatggggagg ggtatgagaa agctccctcc tacagctaca
2641agcagtcctt aaacatggct gccgttggCA TTACGGAGAA CGTGAAGGGA GATGCTAAGA
2701AGTTCGAGAT CTGGTACAAC GCGCGCGAGG AGGTCTACAT CGTCCAGGCG CCAACTCCTG
2761AGATTAAAGC CGCGTGGGTG AATGAAATTC GGAAAGTGCT GACCAGCCAG CTGCAGGCTT
2821GTAGAGAAGC CAGCCAGCAC CGGGCGCTGG AGCAGTCACA GAGCCTGCCC CTGCCGGCCC
2881CGACCAGCAC CAGTCCCTCA AGAGGAAACT CAAGGAACAT CAAGAAGCTG GAAGAAAGGA
2941AAACAGACCC CCTAAGCCTG GAGGGATACG TCAGCTCAGC GCCACTGACA AAGCCCCCCG
3001AAAAGGGCAA AGAGCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
3061TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
3121GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTAGCTT TGATTCAATA
3181TCGGCACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)