Construct: ORF TRCN0000467670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010924.1_s317c1
- Derived from:
- ccsbBroadEn_03929
- DNA Barcode:
- AGTGTACCCACATTATCACCTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TINAGL1 (64129)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467670
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_022164.3 | 100% | 100% | |
| 2 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_001204414.1 | 93.3% | 93.3% | 373_374ins93 |
| 3 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_005271106.3 | 88.4% | 77.2% | 1092_1093ins124;1278_1320del |
| 4 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_011541946.1 | 81.8% | 71% | (many diffs) |
| 5 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | NM_001204415.1 | 77.5% | 77.5% | 0_1ins315 |
| 6 | human | 64129 | TINAGL1 | tubulointerstitial nephriti... | XM_005271107.2 | 77.5% | 77.5% | 0_1ins315 |
| 7 | mouse | 94242 | Tinagl1 | tubulointerstitial nephriti... | NM_001168333.1 | 84.5% | 89% | (many diffs) |
| 8 | mouse | 94242 | Tinagl1 | tubulointerstitial nephriti... | NM_023476.3 | 84.5% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1467
- ORF length:
- 1401
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcgatgtcca ctggggctac tgctgttgct gccgctggct ggccacttgg 121 ctctgggtgc ccagcagggt cgtgggcgcc gggagctagc accgggtctg cacctgcggg 181 gcatccggga cgcgggaggc cggtactgcc aggagcagga cctgtgctgc cgcggccgtg 241 ccgacgactg tgccctgccc tacctgggcg ccatctgtta ctgtgacctc ttctgcaacc 301 gcacggtctc cgactgctgc cctgacttct gggacttctg cctcggcgtg ccaccccctt 361 ttcccccgat ccaaggatgt atgcatggag gtcgtatcta tccagtcttg ggaacgtact 421 gggacaactg taaccgttgc acctgccagg agaacaggca gtggcagtgt gaccaagaac 481 catgcctggt ggatccagac atgatcaaag ccatcaacca gggcaactat ggctggcagg 541 ctgggaacca cagcgccttc tggggcatga ccctggatga gggcattcgc taccgcctgg 601 gcaccatccg cccatcttcc tcggtcatga acatgcatga aatttataca gtgctgaacc 661 caggggaggt gcttcccaca gccttcgagg cctctgagaa gtggcccaac ctgattcatg 721 agcctcttga ccaaggcaac tgtgcaggct cctgggcctt ctccacagca gctgtggcat 781 ccgatcgtgt ctcaatccat tctctgggac acatgacgcc tgtcctgtcg ccccagaacc 841 tgctgtcttg tgacacccac cagcagcagg gctgccgcgg tgggcgtctc gatggtgcct 901 ggtggttcct gcgtcgccga ggggtggtgt ctgaccactg ctaccccttc tcgggccgtg 961 aacgagacga ggctggccct gcgcccccct gtatgatgca cagccgagcc atgggtcggg 1021 gcaagcgcca ggccactgcc cactgcccca acagctatgt taataacaat gacatctacc 1081 aggtcactcc tgtctaccgc ctcggctcca acgacaagga gatcatgaag gagctgatgg 1141 agaatggccc tgtccaagcc ctcatGGAGG TGCATGAGGA CTTCTTCCTA TACAAGGGAG 1201 GCATCTACAG CCACACGCCA GTGAGCCTTG GGAGGCCAGA GAGATACCGC CGGCATGGGA 1261 CCCACTCAGT CAAGATCACA GGATGGGGAG AGGAGACGCT GCCAGATGGA AGGACGCTCA 1321 AATACTGGAC TGCGGCCAAC TCCTGGGGCC CAGCCTGGGG CGAGAGGGGC CACTTCCGCA 1381 TCGTGCGCGG CGTCAATGAG TGCGACATCG AGAGCTTCGT GCTGGGCGTC TGGGGCCGCG 1441 TGGGCATGGA GGACATGGGT CATCACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1501 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1561 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGTGTACC 1621 CACATTATCA CCTCTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1681 aagatt